The author has already informed in the previous report, that the rabbit poisoned by the inhalation of carbon disulphide gas dies without fail by the paralysis of the respiratory centre. This report is based on the results obtained from the research with regard to the determination of the minimal lethal dose in the case of very acute intoxication by the inhalation of evaporated carbon disulphide gas. The experimental method is as follows:
10c.c. that is about 12.7g. of carbon disulphide is put in a small gas washing bottle, which has a capacity of about 100c.c. and is weighing about 80g. The bottle is weighed immediately after the two openings of it are corked closely by means of gum hat. The expiratory opening of the bottle is then connected with the inspiratory opening of the tracheal tube, which is inserted into the trachea of rabbit. Thus the evaporated gas of carbon disulphide in the bottle, together with the air passing through this liquid, is allowed to enter the lung of the animal, which will consequently be poisoned at once. When the respiration of the poisoned animal is ceased, the bottle is quickly taken off from the connection, thereupon the two openings being corked with the gum hat, and is weighed. The difference between the two weights is the total dose, evaporated in the bottle and mixed with the inspired air.
Four of the same gas washing bottle, each of them containing 30c.c. of 10% caustic kali alcoholic solution, are connected with each other by means of gum tube. The inspiratory opening of these bottles on one end is connected with the expiratory opening of the tracheal tube, inserted into the trachea of the animal, and the expiratory opening on the other end is also connected witd a large bottle containing about five litres of water, which flows out in the constant pressure. In this way a negative pressure arises in this large bottle, and it induces the expired air of animal into the latter through the caustic kali alcoholic solution contained in the four gas washing bottles.
The carbon disulphide, while passing through the caustic kali alcoholic solution together with the expired air, is changed into kali xanthogenate and is dissolved in this solution, thereby only the air gets off from it and comes into the large water bottle. The quantity of the kali xanthogenate to be found in the four gas washing bottles is calculated by means of 1/20 normal cupper sulphate solution. The calculated quantity is then substituted from the total quantity consumed as above mentioned, and the remaining corresponds to the absorbed quantity in she blood of animal, that means the minimal lethal dose.
As the water in the large bottle is kept at about the same temperature as that of the experimental chamber, and flows out in the constant pressure so as to make a negative pressure in this bottle as well as in the four connected gas washing bottles, thus inducing the expired air containing carbon disulphide into the bottles, so the volume of the water flowing out from the large bottle corresponds on the whole to the volume of the air (except the carbon disulphide gas contained in it.) inspired by the animal. It has been found by calculation that the quantity of poisonous gas in the inspired air averages about 17vol.%.
From the experiments on nineteen cases the author came to the following results:
The total quantity of the carbon disulphide evaporated in the bottle and mixed in the inspired air of the animal amounts to about 1g. on an average, of which about the half (0.5g.), that is 0.3g. for 1 kilogram of bodily weight, is absorbed into the blood of the animal. This is the real minimal lethal dose pro kilogram of bodily weight in the case of very acute intoxication in the rabbit by the inhalation of carbon disulphide gas. And the animal, inspiring the air which contains about 17vol.% of carbon disulphide dies in only 4 minutes on an average owing to intoxication.

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1. Artificial insemination was performed with two series of female rabbits.
2. Females of the one series, which were inseminated without any other treatment, showed only 8.3 percent fertility.
3. Females of the other series, which were inseminated after copulation with a sterile male, showed 62.5 percent fertility.
4. Duration of pregnancy was one day longer in the latter case than in the former.
5. Thus the proof is fully established that in the rabbit in the majority of cases ovulation does not take place spontaneously, but needs the stimulus of copulation.
6. Successful results with artificial insemination in the rabbit, therefore, can be obtained with a greater degree of certainty if preceded by copulation with a sterile male.

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1. The hemolytic zone, which is produced by the hemolytc streptococcus on the blood agar plate, is influenced by the hydrogen-ion concentration of the medium as well as by use of the blood of the horse or rabbit. It is, however, larger as the sizes of pH increase, i. e. on the acid medium it would be smaller than on the alkaline medium. The optimal alkalinity of the medium as it considered on the prodnction of the hemolytic zone is pH 7.5-8.0.
2. The hemolytic zone of the βg and βm, both of the atypical strains, as it is produced on the horse blood as well as the rabbit blood agar, has an obscure margin irrespectful of the hydrogen ion concentration. It will, however, become more and more clear as the alkalinity is augumented. That of the typical strains, e. g. βt, has a clearly definable margin irrespectful of the reactions of the medium. It is clearer on the rabbit blood agar than on the horse blood agar.
3. The timely relation of the development of the hemolytic zones is: That the time required for the development of the hemolytic zone by βg differs as the horse blood or rabbit blood is employed. On the horse blood agar, having an acid reaction, the hemolytic zone will not cease to grow even after 72 hours of cultivation, but on the alkaline medium, or pH should be 7.5 or more, it will not grow any more after an elapse of 48 hours, the sizes of the hemolytic zone after 72 hours of cultivation will be practically equal to those of the 48 hour old culture. On the rabbit blood agar, Just irrespectful of the reactions as on the horse blood agar, the sizes of the hemolytic zones will grow even after 72 hours of cultivation, the sizes of the zone at the 72nd hour being just eqnal to those of the 48 hour old culture on the horse blood agar.
4. The hemolytic zones of the βm strain will not cease to grow even after an elapse of 72 hours irrespectful of the use of the horse or rabbit blood or of the reactions of the medium. The sizes of the zone on the horse blood agar or on the rabbit blood agar are practically the same.
5. The strain βt, which had been isolated from the normal horse and a strain which had been isolated from glanders produced each different sizes of the hemolytic zones on the horse blood and rabbit blood agar irrespectful of the reactions of the medium, both of them ceased to grow after 48 hours of cultivation. The sizes of the zone after 72 hours of cultivation on the rabbit blood agar will practically be the same as those of the 24 hour old culture on the horse hlood agar.
6. The hemolytic zones on the medium are larger on the horse blord agar than on the rabbit blood agar, which fact may be explained on the differences of the hemolytic power against the homogenous and heterogenous erythrocytes because all the strains I employed for my experiments were isolated from the horse.
7. Instrains the common broth, both typical, e. g. βt and atypical, e. g. βg and βm. proliferated just the same, irrespectful of the differences of the reactions. The longevity of the culture on the medium, however, differs as the reactions of the medium is different. On the medium having a larger pH than 7.5, the differences would become more manifest, because on such a medium βg and βm will live 43-47 days, while the βt strain will live only 3-5 days.
8. The pnoliferation and longevity of both typical and atypical strains on the 1.0-0.5% glucose-brototh agreed with each other. In the 0.1% glucose broth, however, there were met with certain differences concerning the proliferation and longevity of each different strains.
9. Both the typical anb the atypical strains of the hemolytic streptococci of the horse proliferate very good in the common and the glucose broth having the pH of 7.5 and 8.0. Although the pH indices would be a little higher, the same good proliferation would be the result,

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As regards the saccharifying power of the saliva of the horse, various opinions have hitherto been published, but it is now widely known that the power is not a strong one. This has also been proved by the author's investigation. It is generally believed, on the other hand, that the starch digestion which takes place in the stomach of the horse is chiefly due to the action of ptyalin. This contradiction was the motive by which the author began to set his hand to the study of saccharization of food in the stomach. Having made a series of experiments the author has found that the starch digestion in the stomach is by no means due to the action of ptyalin but to an enzyme contained in the bronchial mucus. This enzyme, according to the author's experiment, has a strong saccharifying power and gives rise to the saccharization of food in the stomach. The author called the enzyme "lung amylase" after its origin. The following is the brief description of the experiments.
1) 50c.c. of 1% starch solution, added with 0.25g. of mucus taken from the surface of the tracheal mucous membrane, has been completely saccharized at 37°C in one hour on an average (20 minutes to 3 hours in 13 cases)
2) A piece of lung tissue taken from a horse killed by bloodletting was cut into smaller pieces and extracted with twice as much water as the volume of the piece. 50c.c. of 1% starch solution, added with 5c.c. of the extract, has also been converted into sugar at 37°C in 42 minutes on an average (15 minutes to 2 hours in 9 cases), while it has taken 10 hours and 20 minutes to saccharify the same starch solution by an addition of 5c.c. of horse serum, that means, the saccharifying power of the serum is about 1/150 as weak as that of the extract of lung tissue. From this fact it will be seen that the saccharifying power of the extract is not due to the action of serum amylase.
3) A tracheotomy was performed in the upper and lower part of the trachea and the communication between these two parts was shut putting a tamponade in the middle part. It was noticed, as the result of this operation, that the saccharifying power of the mucous substance taken from the upper part of the trachea became very weak and after 4-24 hours almost disappeared. It has been shown further that even after several hours no increase in the saccharifying power was constated in the mucus taken from the upper part which was then communicated with the lower part by means of a gummi tube to allow the air to pass through it. As for the saccharifying power of the mucus from the lower part no change has been resulted by the operation.
It may be thought from the foregoing that the amylase which is found in the tracheal mucus must be secreted in the lung, because there cannot be found any difference between the histological structure of the upper part and that of the lower part of the trachea.
4) The saliva taken from the oesophagus of a horse, intratracheally injected with a certain quantity of carmin solution, then stimulated to an increased secretion of saliva was coloured with carmin and proved to have a strong saccharifying power. A starch ball which was then given to the horse and taken from the oesophagus was also coloured with carmin. It has been found, moreover, that in the starch chyme which was kept at 37°C for one hour a large quantity of sugar was present. When oat was given, instead of starch ball, it was noticed that an increased quantity of carmin came out of the oesophagus.
The experiments, in which no injection of carmin was performed, gave the similar result, that is, no particular change was observed as regards the saccharifying power of saliva and the sugar formation in the chyme.
5) In the case of a horse in which the trachea was stopped in its upper part so as to prevent the forthcoming of the mucus from the trachea into the pharynx,

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研究ノ結果著者ハ傳染性貧血症ニ於ケル貧血ハ一種ノ溶血性貧血ニシテ赤血球ノ減少スルハ專ラ脾臟骨髄次デ僅微乍ラ淋巴腺ニ於ケル組織性細胞ノ赤血球貧喰作用ニ歸スルモノナリト認メタリ攝取セラレタル赤血球ハへもしでりんニ變化シ然ル後漸次微細ニ崩壞ス脾臟ニ於ケルへもしでりんノ一部ハ恐ラク門脈ニヨリテ肝臟ニ至リ其他ノモノハ身體組織間ニ長ク沈着シへもしでりん或ハ其分解産物ハ容易ニ腎臟或ハ腸管ヨリ排泄セラルルコトナシ終リニ注意ヲ要スルハ熱發作間ニ往々却ツテ赤血球ノ増加ヲ來スコト是ナリコハ病毒ノ作用ニ依ル造血機能ノ異常亢進ニ依ルト見ザルベカラズ然レドモ他方ニ於テ又病毒ノ作用餘リ強烈ナルニ至リテハ造血機能ノ無緊張或ハ衰頽ヲ來シ頑固ナル大貧血ニ陷ルベシ

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Die bekannte Erscheinung, dass das durch Erhitzen inaktivierte Rinderserum, wenn es mit frischem Pferdeserum vermischt ist, also Komplement zusetzt ist, in vitro rote Blutkörperchen zusammengeballt und dann zur Auflösung bringt, hat durch die Untersuchungen von Ehrlich und Bordet und deren Mitarbeiter festgestellt.
Für sich allein kann weder das aktive Pferdeserum noch das inaktivierte Rinderserum eine Zusammenballung der rote Blutkörperchen bewirken.
Ehrlich schreibt dem Rinderserum die Funktion des Ambozeptor zu, durch dessen Vermittelung das Komplement an die rote Blutkörperchen gebunden wird und diese konglutiniert werden. Nach Bordet Ansicht sollen die Blutkröperchen durch das konglutiniert werden, wenn die vorher durch den Ambozeptor des Pferdeserums gebunden haben. Neuerdungs schreibt Miesner vom Mechanismus der Konglutination, die durch 3 Komponente, Konglutinin im Rinderserum, Sensibilisin und Komplement im Pferdeserum hervorgeruft ist.
Diese Reaktion ist schon zur Serodiagnostik praktisch angewandt, obgleich manche Erklärungen auf den Mechanismus imstande sind, und erkannt man die genaue Resultate auf solchen an.
Ich habe zweifelhaft auf oben eräwhnten Beschreibungen, insbesondere vom Eingangsmechanismus nach verschiedenen Autoren, gehabt, so ist es mir wichtig, das festzustellen, welche Erklärung richtig zu sein.
Auf mancherlei Art habe ich meine Versuche ausgefuhrt und diese Resultate sind folgende:
1. Für sich allein kann aktives Pferdeserum eine Zusammenballung der rote Blutkörperchen aus Ziegen bewirken, aber Kaninchen und Meerschweinchenserum keine Wirkung.
In diesem Falle beide Sera ruften die Hämolyse hervor, aber Kaninchen-oder Meersciwehnchenserum konglutiniert rote Blutkörperchen aus Pferde, ohne auszulösen. Es scheint deshalb die Eigenschaft des Serums gegen Blutkörperchen nach der Arten des Tieres verschieden zu sein, wenn man die aus verschiedenen Tieres stammende Blutkörperchen auf denselben Versuche angewandt hat.
2. Beim Eintritt der Konglutination treten inaktiviertes Rinderoder aktives Pferdeserum die Kompensationsfähigkeit mit einander hervor, Kaninchen-und Meerschweinchenserum aber nicht.
Durch Inaktivierungsmanipulation des Serums mit Hitze, die man gewöhnlich braucht, wird Konglutinin zum Konglutinoid verändert, dann bewirkt sich, für sich allein rote Blutkörperchen nicht mehr zusammenzuballen.
3. Wenn man 3 Arten des Serums, inaktiviertes Rinder-und Pferdeserum, aktives Kaninchen-oder Meerschweinchenserum, zum Konglutinationsversuche, ruft heftige Hämolyse hervor, uud man kann keine Kompensationsfahigkeit auf derselben Reaktion erkennen.
Kaninchen-oder Meerschweinchenserum ist Ziegenblutkörperchen zuweilen zu konglutinieren, nach der Beschaffenheit der Individualität.
4. Als man Rinder-oder Pferdeserum zum Konglutinationsversuche braucht, wird die Konglutination positiv geworden, wenn man zum einen inaktiven Serum eine kleine Menge vom anderen aktiven Serum zusetzt. Wenn beide Sera aktiv bleiben, steigt ihre Kompensatsonfähigkeit ca. 10 fach.
5. Komplement, Konglutination zu beteiligen, ist das Endstück desselben Komplement. Obgleich das Enddtück gehaltene Menge des Pferdeserums nicht beständig oder sehr minimum ist, ruft diese Reaktion immer hervor, so kann man annehmen dadurch, dass die Mitarbeit des Kompements und einer im Albuminteil des Pferdeserums enthaltenden Substanz begründet ist.
6. Wenn man das inaktive Rinderserum durch Liefmannsche Mehode Albumin-und Globulinteil trennt, setzt die Fähigkeit des Konglutinoids herab.

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We detected 2 kinds of the spirochete on the ulcerated surface of the prepuce. beneath which was a large round-cell sarcoma of the penis. While I type (Fig. 1) is a flexible spiral 11, 0μ long with 3-7 coarse turns. II type (Fig. 2) reveals an inflexible body 8, 5μ long with 6-15 fine turns. having one minute terminal filament at each end. The spirochete of II type being very closely related to Spirochaeta pallida. the causative agent of syphilis. we on the one hand inoculated materials from the lesion into the testieles of rabbits, and on the other hand we tried the Wassermann test as well as the Sacks-Georgi reaction with the serum of this patient. but all with negative results.
As far as we know concerning spirochetes found by many investigators in a number of ulcers. it may be considered that the spirochetes in question were in all probability non-pathogenic and happened to subsist and multiply saprophytically in the prepuce ulcer produced by the growth of penis sarcoma.

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(1) 粗あすかろんハ主トシテペぷとーん質ヨリ成リ海〓並ビニ馬ニ對スル致死量ハ一頭ニ付キ0.2mg.及1.0mgナリ今粗あすかろんノ濃水溶液ヲ燐うをるふらむ酸ヲ以テ處置シ依テ生ズル沈澱ヲあせとん水ニ可溶ナル部分ト不溶部トノ二區ニ分チ次デ酒精ヲ以テ沈澱シ各々白色ノ粉末トシテ製出スルニあせとん可溶區分ハ其毒力約20倍ニ増加シ其0.01mg.ヲ以テ海〓ヲ斃シ馬ノ致死量ハ0.1mg.ニ達ス反之シテあせとん不溶部ハ其毒力粗あすかうんヨリモ遙ニ弱力ナリ故ニあすかろんハペぷとーんヨリモ更ニ一層ベーすニ近キモノト考フルヲ得ベク又加熱消化等ノ操作ニ堪コル點ヨリ所謂ときしん類トハ餘程異ナレル物質ナルモノノ如シ
(2) あすかろんノ血行器ニ及ボス作用ハ血壓下降ト血管擴張作用ヲ主トス猫ニ於テ血壓ハあすかろん注射後直チニ下降シ又速ニ回復ス而メ下降程度ハ大略あすかろんノ使用量ニ正比例セリ犬ニ於テハ不應性ヲ有スルモノアレ共多クハ血壓ノ下降ヲ招來スあすかろん注射後2-3分ヲ經テ其下降ヲ開始シ直ニみにまむニ達シ10-20分間ニ徐々ニ原血壓ニ復歸スルモノニシテ其一般經過ハペぷとーんしよつくニ酷似セリ
家兎ニ於テハ多量ノあすかろんヲ使用スルモ血壓ノ下降ヲ來サズ之レあなふいらくしーと著シク相異セル點ナリトス血管擴張作用ハ馬ニ於テ顯著ナル反應ニシテ結膜充血浮腫皮濕上昇等ハあすかろん注射後直ニ出現スル處ナリトス剖檢ニ際シ腸壁ニ高度ノ充血並ニ出血ヲ見ル事多シ
蝦蟇心ニ於テあすかろんノ多量使用ニ際シ刺戟傳導系ノ障碍ヲ來タシ搏動停止ヲ來タス又とれんでれんぶるぐ氏後肢灌注法ニヨリ血管壁ニ對スル作用ヲ檢スルニ比較的多量ヲ使用スルモ血管運動ニ影響ヲ及ボサスあとれなりん使用直後ニ於テモあすかろんハ血管壁ニ作用セズ
ひすたみんハ前記何レノ哺乳動物ニテモ確實ニ強度ノ血壓下降ヲ招來ス其經過猫ノあすかろん反應ト同樣ナリ蝦蟇心ニ對シテハ作用極メテ微弱ニシテ搏動増進乃至不整ヲ來タス蝦蟇血管ニ對シテハあんかろん同樣ニ無反應ナリキ
(3) 小氣管枝筋ニ對スルあすかろん作用ハ猫犬及兎ニ於テ極メテ大量ヲ使用スルニ非ンバ大概無効ナリサレドモ海〓ハ極メテ鋭敏ニシテあすからん注射ニヨリ容易ニ氣管枝筋ノ痙攣ヲ來タシ動物ノ窒息死ヲ招來ス
ひすたみんハ前記何レノ試驗動物ニモ極メテ小量ヲ以テ作用シ小氣管枝筋ノ收縮ヲ誘發ス又海〓ニ就テ檢スルニあすかろん注射ニ見ルガ如キ數分ノ潜状期ヲ要セズ注射後直チニ發スルモノトス
(4) 子宮筋ニ對スルあすかろん作用ハ海〓ニ於テノミ確實ニ發現ス然レドモ其効力ハ之ヲひすたみんニ比スルニ頗ル微弱ニシテ大略其1/20ニ過ギズ
(5) 反覆注射ニ對スル抵抗性ノ發生ハ犬ノ血壓下降反應ニ於テ多少發現スルガ如キモ確實ナルモノニ非ズ反之海〓ノ子宮筋及氣管枝筋ニアリテハ常ニ確實ニ發現ス前者ニアリテハ第一回使用量ノ多少ニヨリ1-5倍ノ抵抗性ヲ生ズ
(6) 蛔蟲症ニ於ケル營養障害ヲ蛔蟲毒素ノ作用ナリト唱フルモノアリ今直接ニ蛔蟲粉末ヲ飼料ノ5%ニ添加シ之ヲ授乳中ノ母白鼠ニ與ヘ幼鼠ノ發育ニ及ボス影響ヲ檢シ又タ同上飼料ヲ直接ニ幼鼠ニ攝取セシメ其發育ヲ檢セルニ何レモ對照ニ比シ差異ヲ認ムルヲ得ズ故ニあすかろんハ動物ノ發育ニハ直接影響ヲ及ボサザルモノノ如シ
(7) 内臟寄生蟲粗浸液ノ注射ニヨリテ發スル症状ヲ過敏症ト見做スモノ多シ第一報ニ於テ粗あすかろんノ注射ニヨリテモ粗浸液注射ニ於ケルト同一ノ症状ヲ發スルニ係ラズ粗あすかろんハ過敏症賦與並誘發性ヲ有セザル事ヲ證明セリ諸家ノ使用セル粗浸液中ノ凝固蛋白ニヨリ宿主ハ過敏症ヲ賦與サレ得ルヤ否ヤヲ檢セント欲シ次ノ試驗ヲ行ヘリ
粗あすかろん蛔蟲體腔液同筋壁浸液ヲ以テ鳩ヲ用ヒテ試驗シ體腔液及筋壁浸液ノ過敏症賦與並ニ誘發性アルヲ確メ次デ前二種ノ物質ヲ以テ數回處置セル兎血清ヲ豫メ粗あすかろんノ皮下注射ニヨリ高度ニ抵抗性ヲ有スル海〓腹腔内ニ注射シ次デ前二種物質ヲ以テ被動的過敏症誘發試驗ヲ行ヒ其結果陽性ナルヲ實驗セリ次デ健常馬匹ノ血清ヲ以テ兎血清ヲ以テセルト同様ノ被動性試驗ヲ行ヘルニ共結果一二ノ疑ハシキ場合ヲ除キ陰性ナリキ又馬虻〓水浸液ヲ以テ豫メあすかろん抵抗ヲ與フル事ナク前同樣ノ試驗ヲ施セルニ其結果陰性ナリキ
馬匹ハ蛔蟲浸液又ハ馬虻〓水浸液ノ注射ニ對シ例外ナク強度ノあなふひらくし一樣反應ヲ呈スルモノナルニ係ラズ之等寄生虫ノ蛋白ニ對スル抗體ヲ有セザルモノノ如シ
故ニ寄生虫體浸液ノ注射ニヨル反應ハ大部分あすかろんニヨルあなふひらくといど反應ニシテ眞ノ過敏症ヲ混スル場合ハ頗ル稀ナル場合ト考フルヲ得ベシ

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