かるちうむ鹽類ノ含量ニ乏シク且土類あるかり度ノ著シク低キ飼料 (大麥) ヲ以テ馬ヲ飼養スレバ骨軟症ヲ發生スルモ飼料ニかるちうむ鹽類ヲ補償シテ土類あるかり度ヲ+25mg. ニ高ムレバ本症ノ發生ヲ豫防シ得ルハ本研究ノ第1囘及第2囘報告ノ如シ茲ニ於テ余等ハ更ニ一歩ヲ進メテ Marek ノ提唱ニ從ヒ飼料ノ土類あるかり度ヲ+35mg. ニ高ムルコトニ依リ馬ノ骨軟症ヲ治癒セシムベキ想定ヲ解決シ一方ニ於テハ斯ノ如クかるちうむ及燐酸ノ含量適當ナル飼料ニう゛いたみん (肝油) ヲ添加シ又ハ紫外光線 (水銀石英燈) ヲ照射シタルモノノ效果ヲ檢セムガ爲本實驗ヲ續行シ次ノ成績ヲ得タリ
1. 馬ノ骨軟症ハ沈降炭酸かるちうむヲ添加シ土類あるかり度ヲ+35mg. ニ規正シタル普通飼料ヲ以テ飼養スレバ100日以内ニ概ネ恢復ス此成績ハ實驗的骨軟症ト自然的骨軟症トノ間ニ差異アルヲ見ズ唯症状ノ程度及年齢ニ依リ恢復ニ遲速ノ差アルノミ
2. 飼料ノ土類あるかり度 (かるちうむ及燐酸ノ含量) 適當ナル場合ニ在テハう゛いたみん又ハ紫外光線ヲ應用スルモ其效果顯著ナラズ

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1.臺灣土産ノ黄牛及水牛ノ血尿症ハ從來他ノ牛畜ニ見ラレタル血色素尿症及他ノ原因ニ依テ發スル血尿症ト全ク異ナレリ
2.多數ノ血尿症患牛殊ニ56例ノ解剖的變化ニ據リテ本症ハ病理的變化ヲ原發スル膀胱粘膜ノ出血ニ原因スルモノナリト云フヲ得ベシ
3.膀胱ヲ檢査シテ膀胱炎良性及惡性新生物ノ如キ諸種ノ形態的變化ノ存スルヲ認メタリ而シテ此等ノ變化ハ膀胱粘膜ノ炎症ニ依テ發生シ一旦膀胱炎ヲ發生スルトキハ血尿症ヲ呈シ其原因慢性ナルトキハ新生物ヲ生ズ之ヲ要スルニ血尿症ハ膀胱炎ノ存在ニ因ルモノトス
又一方ニ於テハ此等ノ膀胱腫瘍形成ノ推移ヨリシテ慢性剌戟ガ終ニ諸種ノ腫瘍形成ノ原因タリ得ベキハVirchow氏剌戟説ノ主張スルガ如ク眞ナルベシ
4.すぴろきーた寄生虫卵及でぃぷろこつかすハ本病ノ原因ニアラズ

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牛疫わくちんノ經濟的製造關係ニ於テ加藥ニ石炭酸ヲ試ミタルニおいかりぷつす油及ビとるおーるニ優レル點ヲ認メザルノミナラズ之レガ爲メ却テ免疫元ヲ減殺スル作用アルヲ證明セリ又牛疫わくちんノ基礎液トナル倶里設林ハ比較的高價ナルヲ以テ經濟的見地ヨリ生理的食鹽水ヲ代用シタルニ其ノ試驗成績ニテハ從來製造ニカカル倶里設林水製ノモノニ比シ優レル點ナキモ充分効力アルヲ認ム仍テ應用上價値アルモノト信ズ

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With regard to the etiology of an elephantiasis-like disease known as "Wahi" or "Kose" in our native cattle, I reported in this journal Vol. VI, No. 2 that from the histological point of view the causal agent of this disease was a kind of microfilaria. In this paper I want to state the results obtained by further experiments on this problem.
Although the microfilaria appears in the blood the year round, they are found more numerous in summer. Their appearance in the blood of peripheral veins seems tohave a slight periodicity. A great number of microfilaria are observable at 6-9 o'clock in the afternoon, but it is not so remarkable as in the case of embryos of Filaria bancrofti. The appearance resembles the embryo of a filaria, but the adult worm is not yet decided. Therefore, I have attempted to find them by means of post-mortem examination.
As the result of this examination, many filaria-like worms were discovered in the abdominal cavity of the affected cattle. The worm was long, thread-like in size and white in colour, and the male was smaller than the female. It was attenuated at both ends. The tail of the male was coiled up spirally; that of the female was straight or slightly curved. The uterus contained eggs and embryos in a remarkable number, and the appearance of the latter resembled closely the microfilaria in the blood.
In order to determine whether the embryos can appear in the circulating blood, I put these worms into the abdominal cavity of rabbits. As the result of this experiment I found the microfilaria appeared in the blood of those animals in 3 days after the operation and on the 10th day they were found in maximal number.
Furthermore, I discovered that these worms could be classified into two species by the microscopical examination. One species had strong dorsal and ventral teeth separated by a wide depression from the centre of which arose a semi-circular lip-like elevation. The caudal region of the female was loosely spiral and terminated in a knob-like extremity surrounded by an irregular ring of pointed spines and bearing a pair of lateral appendices close to the extremity. The tail of the male was closely spiral and there were four pairs of preanal and four pairs of postanal papillae, but occasionally one papilla was found closely in front of the cloaca.
The other species closely resembled the former in shape, but the mouth was very small, about one-half the former in diameter, and the posterior extremity of the female terminated in a smooth knob.
From these findings I should like to regard the former, as Boulenger, as Setaria labiato-papillosa and the latter as S. digitata. and to consider that the microfilaria in the blood of affected cattle is closely related to these worms.

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Recently we found 3 inagglutinable strains amongst 19 strains of the glanders bacilli; they are inagglutinable against the anti- No. 7 -strain (of B. mallei)-serum, while they agglutinate in other 18 anti-serums. Such inagglutinability as above mentioned is quitely different in its nature from the ordinarily described inagglutinability. These inagglutinable strains absorb off agglutinins from the anti-No. 7-strain-serum as they do in other 18 antiserums, and anti-inagglutinable-strain-serum agglutinates all 19 strains. Therefore, our 3 inagglutinable strains are agglutinable as other 16 agglutinable strains when they met with serums other than anti-No. 7 strain-serum while they are inagglutinable against anti-No. 7-serum. I intend to explain these phenomena as follows:
In bacterial cell there are receptors which combine the agglutinin to the cell; each of these receptors has corresponding agglutinable substance, and the agglutinin which united with corresponding receptor acts only on the agglutinable substance which belongs to this receptor, thus the agglutination occurs. But, if the agglutinable substance belongs to the receptor which anchored with the agglutinin, or there is some change which is not suitable to agglutinate, or there is something which let the agglutinable substance inagglutinable, or agglutinable substance is deficient, no agglutination occurs.

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