住肉胞子蟲毒素中ニ存在スル血球毒ノ理化學的及血清學的性質ハ從來一般ニ知ラレタル動物性及植物性血球毒ニ對比シ大ニ趣ヲ異シ特ニ溶血毒ノ如キハ浸出法ニ周到ナル注意ヲ拂ハザレバ之ガ證明困難ニシテ更ニ本血球毒ニ對スル血清並ビニ血清各成分就中れちゝん、これすてりん及血清蛋白質等ノ催進作用又ハ阻止作用ニ於テモ蛇毒ノ如キ動物性血球毒トハ異同尠カラザルモノアリ次ニ其要點ヲ抄録スベシ
1.住肉胞子蟲毒素中ニハ2種ノ血球毒即チ溶血毒及血球凝集素存在シ毒單獨ニテ洗滌血球ニ對シテ溶血作用及血球凝集作用ヲ發呈ス
2.溶血作用14種ノ可檢血球中海〓血球ガ最モ鋭敏ニシテ(溶血價6400倍)馬、家兎、犬、豚、人、山羊血球之ニ亞ギ(3200-1600倍)水牛、鷄、鳩、家鴨、緬羊、牛及黄牛血球ハ弱度ノ溶血反應ヲ呈ス(400倍内外)
3.血球凝集作用水牛血球ハ最モ高キ凝集價ヲ示シ(200000倍)人、家鴨及家兎血球ハ次ニ位シ(100,000倍)犬、鳩、馬、海〓、緬羊、牛、黄牛血球ト順次凝集價低下ス(50000-10000倍)
4.兩種血球毒ノ反應ヲ檢測スルニハ2.5-5%ノ血球浮游液適度ナリ血球濃度之ヨリ稀薄ナル時ハ過剩阻止現象ノ圏域擴大シ且ツ血球ハ縱令溶血スルモ游離もくろびん沈澱シテ固有ノ色彩消失スルガ爲メ成績ノ鑑識困難ナリ
5.血球毒ノ溶血作用及血球凝集作用ハ血球種類ニヨリテ其程度ニ著シキ相違アレドモ血球毒ト血球又ハ血球綱材トノ結合力(或ハ吸收力)ハ血球種類トハ無關係ナリ
6.兩種血球毒ノ血球ニ對スル反應速度ハ相均シカラズ即チ血球凝集素ハ比較的迅速ニシテ血球ニ作用後數分間ニシテ現ハレ約2時間ニシテ頂點ニ達スルモ溶血毒ハ30分頃ヨリ徐々ニ反應現ハレ約20時間ニシテ完成ス
7.血球毒ノ理化學的性状兩種血球毒ハ溶解性及耐熱性ニ於テ著シキ相違アリ即チ溶血毒ハあるこーる及ゑーてるニ可溶性ナレドモ生理的食鹽水及ぐりせりん食鹽水ニハ難溶性(恐ラクころいど状ニ浮游シ來ルモノナルベシ)ナルヲ以テ抽出法特ニ磨碎法ヲ丁寧ニスベシ而シテ本溶血毒ハ耐熱性ナレドモ65°30分以上ノ加熱ニヨリテ大部分沈澱物中ニ移行ス血球凝集素ハあるこーる及ゑーてるニ不溶性ニシテ水及生理的食鹽水等ニ可溶性ニシテ85°30分ニテ全ク破壞セラル耐酸性滲透性及被吸着性等ニ就テハ兩種血球毒ハ略相均シク共ニ酸ニ比較的強クあるかりーニ弱クかおりん及動物炭ニヨリテ吸着セラレ動物膜ヲ通過セズ
8.血球毒ニ及ス血清ノ作用健康血清ハ少量ナレバ溶血毒ニ殆ト影響ナキモ一定量以上ヲ混加スレバ溶血作用ヲ全然阻止スルニ至ル該溶血阻止作用ハ同名血清ニテモ異種血清ニテモ大差ナシ而シテ65°-80°度加熱血清ハ溶血阻止作用稍減弱スルモ100°加熱血球ハ却ツテ新鮮血球ト殆ト同程度ノ阻止作用ヲ呈ス
血球凝集素ニ對スル血清ノ作用ハ混加ノ順序ニヨリテ多少相違シ前ニ毒ト血清トヲ作用シ後血球ヲ加フル時ハ凝集價ハ一般ニ著明ノ減弱ヲ來ス
9.血清各成分ノ血球毒ニ對スル作用血清ノあるこーる可溶成分ハ溶血作用ヲ催進シ不溶成分ハ本血球毒ノ溶血作用及血球凝集作用ヲ阻害ス
(イ) 溶血催進物質ノ主體ハれちゝん・ふらくちよんニシテ中性脂肪ハ弱度ノ催進作用ヲ有シけふぁりん及くぉーりんハ本溶血毒ニ對シテ催進作用ヲ呈セズ
(ロ) これすてりんハ本溶血毒ニ對シテ殆ト何等ノ影響ヲ呈セズ
(ハ) 溶血阻止作用ノ主體ハあるぶみん・ふらくちよんニシテぐろぶりん・ふらくちよんハ血球凝集阻止作用ヲ呈ス
10.結合又ハ吸收試驗ノ結果ヨリシテ溶血阻止物質ハ直接ニ溶血毒ニ結合シテ溶血阻止作用ヲ呈シ血球凝集阻止物質ハ先ツ血球ニ結合シ二次的ニ血球凝集阻止作用ヲ呈スルモノト考ヘラル
11.本血球毒ニハ抗元性ヲ認ムルコト能ハズ即チ新鮮毒ふぉるまりん處置毒免疫血清(共ニ毒主成分ニ對シテ抗毒素作用ヲ有ス)及加熱毒免疫血清並ニ本毒素ノあるこーる浸出物ニ豚血清ヲ加ヘテ反覆家兎ニ注射(10-16囘)シテ得タル免疫血清モ抗溶血體及抗血球凝集素ヲ證明スルコト能ハズ但シあるこーる浸出毒免疫血清ハ豚血清ヲ混加スルト否トニ拘ラズ住肉胞子蟲毒素ノ毒主成分ニ對シテ抗毒素作用ヲ有ス

抄録全体を表示

The intraplantar inoculation of guinea-pigs was tried by the author with virus fixe, as done by a number of investigators with viruses of herpes and foot-and-mouth disease. The results obtained are summarized as follows:
1. Inoculated into the planta with virus fixe, the guinea-pig would die from the symptom and course specific to rabies and contain the virus in its brain. The virus fixe thus can be passed through guinea-pigs by intraplantar inoculation.
2. The virus after 5 plantar passages could not be apparently distinguished from the original virus fixe in the neurotropism. The decided opinion regarding this problem, however, must be established from the result of subsequent experiments.
3. Animals resisting the intraplantar inoculation of virus fixe remain always resistant to similar treatments. This may be due to the development of local immunity following the first inoculation.
4. By repeated intraplantar inoculations, the guinea-pig would become immune to the subdural injection of the original virus fixe. Out of 9 animals subjected to 3 treatments, only 1 case was found susceptible to the subsequent subdural inoculation. Such a well developed immunity of central nervous system against virus fixe seems never to have been observed in any of the hitherto known antirabic treatments.
5. In guinea-pigs repeatedly treated, if they succumbed to the subdural inoculation of virus fixe, the interval between dates of inoculation and death tends to be prolonged. This may also prove the limited development of antirabic immunity in the central nervous system.
6. Such immunity of central nervous system seems to disappear in a half year. The conclusion regarding its duration must be, however, positively determined by the further experiments.
7. Virulicidal principles are detected in the serum of guinea-pigs thus immunized against virus fixe.
8. The guinea-pig is also susceptible to the intraplantar inoculation of "street virus."
9. The author failed to detect the living virus in the brain of 1 guinea-pig, which succumbed to the subdural injection of virus fixe carried out 13 days after 3 intraplantar inoculations. It may probably be one example of "neuro-infection auto-stérilisable" (Levaditi).

抄録全体を表示

狂犬病ニ於ケル補體結合反應ニ就キテハ古來其ノ報告尠シトセズHeller & Tomarkin,Friedberger等ガ陰性ヲ主張スル其ノ半面ニ於テハNedrigailoff & Sawtschenko,Zell等ノ陽性論ヲ主張スルモノアリ未ダ其ノ賛否確認セラルルニ至ラズシテ時ノ推移ニ委セタルノ状態ニアリシガ近時Kraus & Michalka等ノ陽性論ヲ新タニシタルニヨリ再ビ之ニ關スル報告ヲ加フルニ至レリ余等モ曩ニ此ノ試驗ヲ企圖シ各種ノ方法ヲ以テ家兎ヲ免疫セシメ其ノ血清及ビ狂犬病竝健常あんちげんトヲ以テ多數ノ實驗ヲ行ヒタリ其ノ結果下ノ結論ヲ得タリ
1.固定毒又ハ市街毒ヲ以テ免疫セシメタル家兎血清ハ狂犬病あんちげんヲ以テスルモ將又健康家兎及犬腦ヨリ製セル所謂健常あんちげんヲ以テスル場合モ屡々補體ヲ結合ス
2.健康家兎又ハ健康犬血清ニ於テモ亦同樣兩あんちげんヲ以テ屡々補體ヲ吸收ス
3.免疫血清及ビ健康血清兩者ニ發現スル反應ノ程度ニ格別ノ差違ヲ認メ得ズ且ツ鹽酸法ニヨル非特異物質ノ除去ニヨリ兩者共一樣ニ其ノ反應ヲ減退又ハ消失ス
4.從テ狂犬病免疫血清ニ於テ屡々發現スベキ補體結合ノ現象ハ特異性ヲ有スルモノニアラズ即チ狂犬病免疫血清ノ補體結合反應ハ陰性ナリトス之ヲ換言スレバ狂犬病々毒自身ハ動物體内ニ於テ特異性補體結合素ヲ産生セシムベキ能力ニ乏シキモノナリト謂フヲ得ベシ

抄録全体を表示

The author found a female elephant louse on an Indian elephant at Maruyama Zoological Garden in Taihoku last January. That is closely related in most important points to Haematomyzus elephantis which was found by Piaget.
Only differences between both specimens are as follows:
1. There are four double hooks on the posterior part of proboscis tip, instead of three in Piaget's specimen.
2. Four ridges are formed lengthwise on the surface of occiput, while Piaget's specimen has three ridges in the same place.
3. There are brownish shieldform or heartshaped blotches along the margins of six anterior segments of the abdomen. Piaget's specimen has subquadrangular blotches.
4. There are eight rows of nipple-like oval process on the ventral surface. The first and second rows are located on the thorax and connect in their both ends. The Processes on the first row are nine in number, and on the second row are eighteen.

抄録全体を表示