The supernumerary chromosomes f_l in 2n=24+1f_l plants of Lilium callosum were found lying on the micropylar side (63.7%), on the chalazal side (13.0%), and on the MI plate (23.2%) at MI in EMCs, the disposition of f_l univalent not being a random one. If the f_l outside the MI plate passes undivided to the closer pole and the f_l on the MI plate passes to either pole, micropylar or chalazal, with equal chance, at least 75.4 per cent of micropylar nuclei will include 1f_l. Actually, at AI, MII, AII, the post-meiotic 1st and 2nd divisions, 80.0 per cent of the micropylar nuclei showed n=12+1f_l. This value coincides exactly with the result of the progeny analysis of the cross (24+1f_l)×(24) which gave 2n=24+1f_l and 2n=24 in an approximate proportion of 80:20 in percentage.

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1. For materials, eggs of White Leghorn, Rhode Island Reds, and Nagoyas were used. The female sex hormone, estradiol benzoyl ester, was injected into eggs as near as possible to the embryos on the fifth day of incubation.
2. The minimum quantity of the hormone which brought about transformation of the testis into an ovary was determined.
3. In two inbred lines of White Leghorns 600 I. U. of female sex hormone was proved to be the minimum dosage necessary to bring about transformation of the testis into an ovary. In two inbred lines of Rhode Island Reds more than 1000 I. U. of the hormone was required to induce transformation of the testis to a degree similar to the inbred lines of the White Leghorns. The same results were obtained with the Japanese breed Nagoyas as with the Rhode Island Reds.
4. The authors intend to interpret the difference in response of the gonads to the female sex hormone among various breeds as the difference of hereditary sex determination among these breeds.

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The author modified the technic for the agglutination-lysis and the agglutinin-absorption tests. He also adopted a new notation system to express the results of the former test. These contributions are believed to facilitate the serological studies on leptospires almost as well as on other microorganisms. These modifications are playing an important role in the recent advancement of research of leptospirosis in Japan.

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The effect of bacterial adrenaline sensitizer (B.A.S.), (a polysaccharide fraction extracted from the cell body of shigella flexneri 2b isolated from a patient with malignant syndrome of “Ekiri”), on the central effect of l-adrenaline, l-noradrenaline, and serotonin was studied using Marrazzi's transcallosal preparation of rabbits.
B.A.S., which was shown in another experiment²⁾ to sensitize the blood vessel to l-adrenaline and l-noradrenaline but not to serotonin, lowered the threshold value of not only l-adrenaline and l-noradrenaline but of serotonin for their inhibition of callosal potential and also augmented the effect.
In the unanesthetized preparations the reduction of callosal potential by these catecholamines took place with the desynchronization of the background activity, but after the B.A.S. administration it is followed not only by the desynchronization but sometimes by the synchronization. In the anesthetized animal the threshold value for the reduction of callosal potential by those catecholamines was heightened as compared with the cases of unanesthetized animals and was not always followed by the change of the background activity.
The clinical significance in the so-called infectious toxic encephalopathy of those catecholamines and B.A.S. was discussed.

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