The chromosome constitutions of three cases of bovine freemartins were observed in blood cultures as well as in tissue cultures from various organs. In both freemartins and their co-twins (case A-C), tissue cells of mesodermal and endodermal origin generally showed 2A-
XX/2A-
XY mosaicism. A normal cow from the non-freemartin dizygotic twins possessed a normal female complement of chromosomes (case D).
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