1. When
OLe(a+b-) red cells or crude glycolipid extracted from them were incubated with Le
a-decomposing enzyme from
Bac. cereus Lea, fucose and galactose were liberated, as the result abolishing Le
a activity. In this case, about 8.4% of the original amount of fucose and about 13.5% of the original amount of galactose were liberated from the red cells, and about 11.1% of the original amount of fucose and about 4.1% of the original amount of galactose from the crude glycolipid. Incubation of
OLe(a-b+) red cells with this enzyme preparation resulted in the liberation of a small amount of galactose unrelated to the blood group specificity.
2. From the amount of fucose liberated from red cells by the enzyme action, the molecule number of fucose attached to one red cell as the determinant of the Le
a specificity was computed.
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