1) It was found that dialysable fragment having peptic activity could be obtained with a high yield by dialytic autolysis of crude pepsin preparation at 3-5° in acetate buffer solution of pH 4 and ionic strength 0.1.
2) The temperature of the incubation and pH as well as ionic strength of the solution governed the formation of the active fragments.
3) Three fractions having activity were separated by column chromatography of the dialysate using amberite IRC 50 (XE 64).
4) A fraction containing almost a half of the activities of the eluate was revealed to be a mixture of small molecular polypeptides, of which each of the molecular weights was assumed to be smaller than 10, 000 by measuring the sedimentation constant.
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