Localized scleroderma is an autoimmune disorder characterized by sclerosis of the skin with exclusive cutaneous involvement, while the present research on treatment is mainly through oral or injected methods, which is systemic delivery causing side effects and injection is with administration difficulty and pain. In an effort to develop a new therapeutic method to treat localized scleroderma, we have designed a Hyaluronic Acid (HA) biodegradable microneedle patch that encapsulates imatinib mesylate. The proposed local delivery method overcomes side effects with systemic delivery with advantages of microneedle as high efficiency, no pain. Imatinib mesylate enables treatment of scleroderma through the inhibition of the TGF-β, which consequently results in a decrease in collagen production.The microneedle patch is fabricated through the micro-molding method with solution of HA and Imatinib mesylate as the material. The female dimethylpolysiloxane (PDMS) mold with different height is made by a metal master mold and stage controller. We fabricate microneedle with different heights and dose of drug. After fabrication, we have ensured the safety and drug activity after fabrication through cell test and in the following step we will carry out in vivo experiment to test the feasibility and stability of the new treatment method.

Microneedles (MNs) that penetrate skin surface to administer drugs in minimally invasive manner, have been focused and developed in recent years. In this research, a fabrication method for whole-dissolvable MNs patch in large area is investigated. A stamping and drawing method with a 3D-printed hydrophilic polymer stamp is proposed to manufacture MNs patch in large area simply and rapidly. Firstly, the Poly(methylmethacrylate) (PMMA) stamps were designed as an array of 81 pillars (diameter: 1mm) on the plate (5.76 cm2) and formed in a few minutes by 3D printing. Hyaluronic acid (HA, 15 wt%), a biodegradable polymer that can solidify at room temperature was utilized as matrix material to fabricate dissolvable microneedles. Rhodamine B (0.15 wt%), a fluorescent dye was used as model drug. The substrate material was Poly(vinyl alcohol) (PVA) that is water-soluble and can be dissolved with a few drops of water and thus, there is no need to peel off the patch after MNs patch application. The large size MNs patch (25cm2) with drug-loaded was manufactured by repeating the stamping process by 4 times. The results show that the height of MNs was 576 ± 92 μm and the tip diameter was 38 ± 17 μm, respectively that met the requirement of painless transdermal drug delivery. The model drug distributed uniformly in the microneedle measured by confocal fluorescent microscope.

Minimally invasive insertion of needles offers considerable advantages over the conventional sensing routes. Moreover sensing at transdermal region is easily accessible without any complications. Measurement of electrolyte abnormalities resulting from prolonged sweating would be easiest way to detect the heatstroke risk. In this regard, we proposed a low cost, facile preparation, easy to use sensor for the detection of ions in transdermal region. Microneedles was achieved by replicating structures from a laser engraved stainless steel master. And then PDMS elastomer was poured on stainless steel master, cured for 2 hours at 70oC and peeled off. The peeled off microneedle array structure is semi flexible and transparent. Then, Au/Ti electrodes were deposited on PDMS using shadow mask to make electrodes for sensing studies. Finally the electrodes were modified using Ag/AgCl and ZnO for sensing characterizations. Electrochemical studies were performed using the conventional three electrode system by employing electrochemical workstation (Bipotentiosrat 2325, ALS Japan). Cyclic voltammetric and amperometric techniques were used for electrochemical analysis of the bare and metal oxide functionalized microneedle electrodes in the range of 10-100 μM.

Processing technology for manufacturing fine devices and next-generation functional parts are demanded. In this study, by focusing on the optical trapping potential due to the electric field intensity gradient, we aimed to process three-dimensional nanostructures with a resolution in a range smaller than 100 nm by localizing and three-dimensionally accumulating nanoparticles without being limited by the diffraction limit. In this paper, we have proposed a new method to achieve the above purpose and experimentally verified the proposed method.

Sculptured surface on large-scale dies with complex curved surface such as automobile body parts is usually fabricated by ball-end-mill using 3-axis NC milling machines. Normally, toolpath for machining products is generated by geometric calculation between product model and tool. However, if the product has a large and complex surface, number of polyhedrons approximating the product increases, so a calculation time becomes enormous. In past research, methods of geometric calculation using offset surface generation and the parallel processing function of GPU were proposed to solve this problem. However, these methods didn’t consider error due to the approximation process and increase in memory usage. To reduce this geometric error and memory usage, we propose a new method for accurate offset surface generation. In this method, the offset surface is generated by vertices of the polyhedron constituting the product and the tool radius. This approach enables accurately calculating a height of the offset surface of the area approximated by the fine polygon shape while suppressing an increase in memory usage.

In early lung cancer surgery, the surgeon scooped the organ surface at the surgery, and decided the removal range while confirming the existence of the tumor. However, because the spread of endoscopic surgery has made it difficult to directly touch the organ surface, a endoscope-like instrument is required. Therefore, an endoscope with a pressure sensor that presents the existence of the tumor mass to the surgeon has been developed with a mechanical pickup.

We have fabricated a three-layer transparent device, that will encapsulate single cells and eject them in parallel after encapsulation. Micro device consists of three layers: a) Cell channel, that includes the cell path and trapping chamber, where cell will be trapped because of overhang structure and specific design of device. b) Pneumatic channel, pressure will be applied via pneumatic channel once cells are encapsulated in trapped chamber, and this applied pressure will deflect the variable membrane that work as valve. c) Openings, opening are designed next to trapping chamber and can be actuated by the deflection of variable membrane. openings are made for parallel ejection of encapsulated cells. Since this device is made of SU8 and PDMS hence it is transparent. Because of its transparency, cell and liquid can be observed in real time.To encapsulate the single cells, first cell will be flown into the cell channel at this time opening will be kept closed by deflection of variable membrane, now once cells are trapped into the channel, oil will be flown to the same direction to encapsulate the cells and after encapsulation opening will be opened and cell will be ejected parallel.