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Gaurav Pandey, Rohit Bhardwaj, Kentaro Tanagi, Takayuki Shibata, Moeto ...
Pages
281-282
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Picolitre pipetting plays a critical role in micro-biology and material science because of its ability to deliver precise volumes of multiple chemicals and reagents into well plates for subsequent analysis. The purpose of this paper is to develop a microfluidic liquid handler, picolitre dispenser array, which can dispense picolitre droplets of biomaterials containing cells, particles, and protein etc. A pneumatically controlled nozzle array provides high-throughput parallel ejection of liquid and enables dispensing. A flow inside channels was observed with transparent structure of a nozzle array, and the ejection was controlled by the integration of a pneumatic channel. A nozzle array was placed on a well plate with customized stages and the dispensing of liquid was characterized by changing a pneumatic pressure.
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Yoshiyuki Oyama, Takuya Kohno, Azusa Kage, Takayuki Shibata, Moeto Nag ...
Pages
283-284
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Harsh Gupta, Naoki Iwasaki, Shin Sawai, Pallavi Shinde, Tuhin Subhra S ...
Pages
285-286
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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To advance intranuclear delivery of human cells, ns-pulsed laser was used for optoporation. Optical transfection techniques are less-invasive, clean, and efficient, hence optoporation is chosen. After growing cells on the substrate coated with a metallic layer of Titanium at specified micro-places, those are irradiated with laser using different energies. The goal is to optimize laser parameters for the same. Viability test and delivery rate is determined for characterization. Calcein AM and PI dye is used for viability test and FITC-dextran and PI dye is used to determine delivery rate. The study finds its major application in pharmaceutical industries, customization of medicines and drug discovery.
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High-precision pulsed-current measurement of high-impedance microdroplet
Miho Ishii-Teshima, Siti Syuhada Binti Azhar, Hirofumi Kurita, Rika Nu ...
Pages
287-288
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Kentaro Takao, Qi-wen Tang, Takeshi Hatsuzawa
Pages
289-290
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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For the investigation on dry eye mechanism, a method to measure the contact condition with the pressure of the eyelid is required. A new method to measure the contact force is proposed, in which a gel-like substance with high biocompatibility is spin-coated resulting in the color density. An experimental evaluation on the mechanical properties of gelatin coated membrane is also described.
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Takeru Fukunaga, Yuya Suzuki, Azusa Kage, Takayuki Shibata, Moeto Naga ...
Pages
291-292
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Tatsuya Yoshizawa, Yuki Jingu, Yuki Oka, Masaru Irita, Toshihiro Suzuk ...
Pages
293
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Guangchong Ji, Naotomo Tottori, Takashi Nishisako
Pages
294-295
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Separation of lactic acid bacteria from a heterogeneous population is an important step for sample preparation in industrial and biological assays. Recently, particles separation using microfluidic devices has attracted attention, and among these techniques, deterministic lateral displacement (DLD), which is one of the passive separation techniques, has been applied for separation of various particles in micro/nanometer scale. However, to the best of our knowledge, separation of lactic acid bacteria using a DLD device has not been reported. In this study, we have demonstrated separation of lactic acid bacteria (size: 3–13 μm) from yogurt solution through polydimethylsiloxane (PDMS)-based DLD micropillar arrays (pillars diameter:12.5 μm; the gap between the posts: 7.5 μm, 10 μm). The diluted yogurt sample with phosphate-buffered saline (PBS) solution (dilution factor:100 fold) was infused into the DLD array with sheath fluid stream of PBS solution, and the trajectories of fluorescently stained bacteria flowing through the DLD arrays were evaluated.
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Yuko Sato, Norichika Tomida, Akitaka Nagamine, Masaru Irita, Shuhei Og ...
Pages
296
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Tomoki Hosaka, Takashi Nishisako
Pages
297-298
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Shun Muramatsu, Takashi Masuda, Seiichi Takamatsu, Toshihiro Itoh
Pages
299-300
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Yohei Kato, Arata Kaneko
Pages
301-302
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Kazuki Arai, Miki Okawa, Hitomi Motomura, Masaru Irita, Kazunori Akimo ...
Pages
303
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Kodai Kawaguchi, Kenta Kato, Arata Kaneko
Pages
304-305
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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4th Report: Study of Fiber Diameter Control Method
Ryosuke Iinuma, Nobuhide Itoh, Taiki Yamamoto, Satoshi Kashimura, Shin ...
Pages
306-307
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Naoki Mori, Nobuhide Itoh, Toui Yano, Katsufumi Inazawa, Hitoshi Ohmor ...
Pages
308-309
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Katsufumi Inazawa, Takeru Sakamoto, Atsushi Ezura, Hitoshi Ohmori, Nob ...
Pages
310-311
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Hitoshi Nishida
Pages
312-313
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Hitoshi Nishida, Hisashi Yamamoto, Kunio Shimada, Yasushi Ido, Noboru ...
Pages
314-315
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Hideki Kawakubo, Unkai Sato, Shuichi Murata
Pages
316-317
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Investigation of finishing mechanism and the precision finishing of aluminum alloy plate
Yanhua Zou, Huijun Xie
Pages
318-319
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Kentaro Omori, Nozomu Fujimoto, Takefumi Kanda, Syuichi Wakimoto, Nori ...
Pages
320-321
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Ryo Arakawa, Shigeki Mori, Akihiro Naganawa, Yotsugi Shibuya, Goro Obi ...
Pages
322-323
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Kang Chen, Takasuke Irie, Takashi Iijima, Takeshi Morita
Pages
324-325
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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To ease the potential bioeffects including side burnings and haemorrhage of tissues induced by conventional High Intensity Focused Ultrasound (HIFU) treatments and to achieve powerful ultrasound transmission using Minimally Invasive Treatments (MIT), we invented Double Parabolic refLectors wave-guided high-power Ultrasonic tranSducers (DPLUS). Powerful ultrasound is generated by acoustic focusing of the 1st parabolic reflector and guided by the 2nd parabolic reflector, the resulted plane-wavefront powerful ultrasound is propagating through the miniaturized cylindrical waveguides for high power transmission. Simulation, analytical results and experiments positively support the high-power ultrasound generation. Experimental results indicate that extremely large vibration velocity at the waveguide tip can be achieved. Vibration velocity peak value of 5.2 m/s can be realized at around 140 Vpp, 0.814 MHz, and amplification ratio of vibration velocity reaches up to around 23 under 5-cycles burst excitation. The superior performances are promising for enormous biomedical applications, including ultrasound therapeutics, cell capture, cavitation, etc.
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Qingyang Liu, Kang Chen, Junhui Hu, Takeshi Morita
Pages
326-327
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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The ultrasonic manipulation technology has drawn widely attention recently due to efficient manipulation process. This report presents a high frequency operation to manipulate micro objects in the water, by ultrasonic tweezers. A low-loss and high-power ultrasonic device DPLUS (Double Parabolic reflectors wave-guided high-power ultrasonic transducer) was used as the ultrasonic tweezers. During the manipulation, a needle probe of DPLUS, which vibrates along the axis of needle, was immersed in the water and vertical to the substrate. When the distance between needle tip and substrate was a certain value, micro objects could be suspended between the substrate and vibrating needle tip, and transported by moving the vibrating needle horizontally. In our experiments, micro object samples include single copper wire with a diameter 60 μm and length of 0.5-2 mm, the cluster of yeast cell with a diameter about 15 μm (single cell has a diameter of 3-10 μm). With finite element analyses about the distribution of sound pressure, it was clarified the copper wires and cluster of yeast cells are suspended near the sound pressure nodal plane of the standing wave excited between the substrate and needle tip.
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Takumi Nishida, Takefumi Kanda, Shuichi Wakimoto
Pages
328-329
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Susumu Miyake, Tomohiro Harada, Hiroyuki Shimizu, Sumiaki Kishimoto, T ...
Pages
330-331
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Akira Iwasaki, Takeshi Morita
Pages
332-333
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Takahiko Iwase, Hiroyuki Shima, Nobuhiko Henmi
Pages
334-335
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Nobuhiko Henmi
Pages
336-337
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Zhuo Chen, Xiaoyou Zhang
Pages
338
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Shunsuke Izuhara, Zhang Zhong, Tomoaki Mashimo
Pages
339-340
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Daisuke Yamaguchi, Ryo Nagami, Yuji Ishino, Masaya Takasaki, Takeshi M ...
Pages
341
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Hiroshi Kimura, Masaya Takasaki, Daisuke Yamaguchi, Yuji Ishino, Takes ...
Pages
342-343
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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1st Report: Fabrication of the ultrasonic transducers and basic experiments
Masahiro Kubota, Masaya Takasaki, Daisuke Yamaguchi, Yuji Ishino, Take ...
Pages
344-345
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Haruto Takami, Takefumi Kanda, Shuichi Wakimoto, Hikaru Yamamoto, Koic ...
Pages
346-347
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Hiroaki Miyake, Akihiro Torii, Suguru Mototani, Kae Doki
Pages
348
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Ayaka Nakazato, Akihiro Torii, Suguru Mototani, Kae Doki
Pages
349-350
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Yuuma Tamaru, Kensuke Kawada, Hiroki Shimizu
Pages
351-352
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Takamitsu Kaneko, Yuichiro Orino, Takeshi Morita
Pages
353-354
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Takashi Mizoguchi, Takaya Nagahama, Takayuki Yoshimi, Makiko Yonehara, ...
Pages
355-356
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Hiroki Ninomiya, Tatsuya Furuki, Hiroyuki Kousaka
Pages
357-358
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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The demand of metal additive manufacturing (AM) has been increased as a high-flexibility manufacturing method that can fabricate a complex shape. However, the metal AM has following problems; the influences of molding condition on the material properties such as hardness or porosity is not clear. A decision of machining condition is difficult since it is impossible to estimate a machining behavior that may occur on cutting or grinding. In this report, the influences of molding condition for metal additive manufactured maraging steel on material properties and grinding characteristics were investigated.
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Masataka Chuzenji, Shinji Ishibashi, Takumi Mizoi, Hidekazu Maehana, K ...
Pages
359-360
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Kengo Fujimaki, Hiroyuki Chiba, Satoshi Okubo
Pages
361
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Hiroyuki Chiba, Kengo Fujimaki, Satoshi Okubo
Pages
362
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Amane Nagumo, Tomoaki Ario, Daisuke Matsuura, Yusuke Sugahara, Yukio T ...
Pages
363-364
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Takashi Kigure, Yuki Yamauchi, Toshiki Niino
Pages
365-366
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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Yuki Yamauchi, Takashi Kigure, Toshiki Niino
Pages
367-368
Published: August 20, 2019
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Atsushi Ishikawa, Toshiki Niino
Pages
369-370
Published: August 20, 2019
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Ryuichi Kobayashi, Masashi Hagiwara
Pages
371-372
Published: August 20, 2019
Released on J-STAGE: February 20, 2020
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