In order to establish a simple, sensitive and rapid method for the determination of azithromycin, the gold electrode modified with Silica sol/Nano-Au/PVP/Ru(bpy)
32+ was prepared, and the electrochemiluminescence (ECL) method was developed for the determination of azithromycin based on the sensitizing effect of azithromycin on Ru(bpy)
32+. Moreover, the electrochemical behaviors and the electrochemical luminescence behaviors of the Ru(bpy)
32+ system and the Ru(bpy)
32+- azithromycin system were investigated by ECL respectively. The results show that the proposed electrode showed very good electrochemical activity and electrochemiluminescence response to the Ru(bpy)
32+- azithromycin system, under the optimum experimental condition, the linearity of azithromycin concentration to ECL intensity was achieved in the concentration of azithromycin from 1.0 × 10
−7 to 1.0 × 10
−4 mol L
−1 and the detection limit (
S/
N = 3) was 1.33 × 10
−9 mol L
−1. Through the parallel determination of 1.0 × 10
−5 mol L
−1 azithromycin for 8 times, the RSD of results was 1.82%, and the recovery ranging from 97.9% to 101.6% was obtained in the detection of azithromycin tabelets and the RSD was 1.36% (
n = 5). The proposed method showed high selectivity and sensitivity and simplicity, and it could be applied to the analysis of azithromycin with satisfactory results.
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