This paper investigated on the electrophysiological aspects of masseter muscle in patients with progressive muscle dystropy.

These Findings were as follow:

From the electromyogram of masseter muscle obtained by surface electrodes, it was recognized that the muscle activity showed low amplitude action potential, prolonged interval of pulse occuring, low frequency component on EMG power spectrum, small integral count and prolonged duration of silent period.

From these findings, it was suggested that the muscle atrophy in masseter muscle according to partial disappear of muscle fiber was appeared similarly to skeltal muscles.

The tooth germs of first mandibular molar from 3-day-old mouse were used. In order to eradicate the basal lamina, the predentin of tooth germs were mechanically removed. After the separation of tooth components, the recombined epithelial and mesodermal components were incubated in a humidified incubator at 37°C in an atmosphere of 5% CO₂, 45% N₂ and 50% O₂. A daily change of regeneration of new basal lamina was observed by electron microscope.

The non-periodic fine fibrils which were deposited on the epithelial cell surface increased as the time of cultivation prior to formation of new basal lamina. The new basal lamina deposited at five days after the cultivation. These results suggest that non-periodic fine fibrls have a close association with the regeneration of basal lamina.

The anatomic structure that collectively form the face normally developed concurrently and are interdependent during function throughout life. In this homostatic complex, disruptive events such as removal of the natural teeth affect the form and function of remaining living Parts.

The proper position of artificial teeth allow edentulous patients to preserve their facial identity as it existed when the natural teeth were present. Therefore, creating facial and function harmony with artificial teeth becomes a biologic challenge of utmost significance for the prosthodontists. Moiré topographic study has been made to analize to aid for edentulous patient’s facial esthetics.

From the results, following conclusion can be made;

1. Facial contour can be classified as three types.

2. Zygomatic contour as a guide for restoration of the facial esthetics for the edentulous patient’s can be applied.

3. Correlation between upper lip contours and dental arch form are existed.

4. Female facial contours are more likely rounded than male.

For the purpose of clarifing their ecology, Haemophili which inhabit the upper respiratory tracts, were isolated from saliva and dental plaque of 38 heahhy human adults. The isolates were then classified by biochemical properties, and were also tested for their sensitivity to antibiotics.

The average counts of Haemophilus in the saliva and that in dental plaque were 6.6×10⁶/ml and 6.0x10⁵/g, raspectively.

Three hundred sixteen isolates were analyzed by biochemical properties. Eleven were identified as H. influenzae which were found only in saliva and then belonged to biotypes I~V, but not to Vl. Two hundred twenty-nine isolates were identified as H. parainfluenzae. Of those biotype I and Ⅲ were found in both saliva and dental plaque, but biotype Ⅱ was found only in saliva. Sixty four H. paraphrophilus were isolated from both saliva and dental plaque. Five isolates H. aphrophilus were found only in dental plaque, and 7 H. parahaemolyticus only in saliva.

One hundred one strains comprising H. influenzae, H. parainfluenzae, H. aphrophilus, H. paraphrophilus and H. parahaemolyticus were tested for using different 20 antibiotics. Among 6 penicillins, PCG showed weak antibacterial activity, however PIPC, APPC, MZPC exerted very strong antibacterial activity. Among 8 cepharosporins CZX exhibited strong antibacterial activity and its peak minimum inhibitory concentration (MIC) was 0.5μg/ml.

Some isolates showed high resistance to PCG, ABPC, SBPC, CXM, CZX, CEX or CCL (their MIC being≧100μg/ml), The penicillin-resistance strains produced β-lactamase.

The small explants (0.5mm³ cuboid) of sarcoma induced by MC in C3H/HeN mouse were transplanted syngeneically under the serosa of the spleen. The recipient mice were devided into two groups. One was young group (two-month-old) and the other was old one (eleven-month-old). Transplanted tumor tissue survived in all recipient mice. Seven days after transplantation tumor tissue grew protruding from splenic surface and also infiltrated deeply into splenic parenchyma. Fifty three days after transplantation, the whole spleen was replaced by conglomerated tumor mass. Tumor growth was more conspicuous in young group of mice than in old one. Histologically, the boundary between transplanted tumor tissue and splenic parenchyma was progressively cleared, but any membraneous structure was not ultimately formed. Many plasma cells and lymphocytes were directly contact with tumor cell and no specialized cellular struture was formed between them. In part which showed tumor cells and lymphocytes were in direct contact, simultaneously plasma membrane of tumor cell became unclear. There were no prominent histological difference between young group of mice and old one.

Electron microscopic study was made on the liver obtained from an autopsy case with primary biliary cirrhosis (PBC), in cirrhotic stage. In the study, intranuclear inclusions were found in part of infiltrating lymphocyte and hepatocyte, and nature of these inclusions were reported. Intranuclear inclusions, which were found in lymphocyte, had crystalline structure showing regular periodicity. This structure closely resembled the immunoglobulin-crystals which were well known crystalloid inclusion body found in the cases of multiple myeloma and Waldenström’s macroglobulinemia. It was worthy of note, because PBC was also associated with hyper-gammaglobulinemia, and frequently showed autoimmune disorders. Intranuclear crystalline inclusion found in the present study suggested morphologically immunological disorder in PBC. Another intranuclear inclusion, which had double-layered limiting membrane, was present in the hepatocyte. Although this intranuclear illclusion was not specific finding in PBC, it was in some way related to the productive processes of the hepatocyte, since it was similar in morphologic characteristics to inclusion found in the secretory cells of the salivary glands.

This report is the rare case of central tubercles appearing bilaterally on the first and second premolars of upper and lower jaws of a 10 year old male.

The measurement of the crowns of the remaining teeth is larger than Japanese average by Kamijoh.

The central tubercles is localized at the buccal trianglar ridge. Pulp cavity invaded into the central tubercles.

The result of investigation of his family was as follows, projection of cusp, swelling of trianglar ridge, and tuberclar prominence at the trianglar ridge on the premolar tooth.

This syndrome was described by Rubinstein and Taybi in 1963. As yet seventy five cases were reported in Japan, but there are few reports described on the dental findings. The oral findings and its treatment were reported in this study. The clinical characteristic features of the Rubinstein-Taybi syndrome were almost observed in this case. Especially, according to the oral findings, the highly arched palate, undergrowth of the both jaw, the remarkable crowded teeth and intense marginal gingivitis showed in this case. Further, this case showed the characteristic features as upper supernumerary incisors, anterior and posterior cross bite and missing of upper right premolar. But in this case, the malformed tooth and imperfect tooth were not presented. The care for the mental retardation and the constant acute respiratory disease are nessesary in treatment with the syndrome.

We made a plan of the treatment as follows,

1)Extraction of ectopic teeth in order to prevent from gingivitis due to markedly crowded teeth and multiple caries.

2)Conservation treatment of caries.

3)Tooth-brushing instruction for gingivitis.

4)Expansion of maxilla for cross bite.

The above plan is almost successful.