This study was followed:1. Analysis of the chemical composition of eight species(Eucalyptus propinqua, E.punctata, E.tereticornis, E.viminalis, E.camaldulensis, E.microcorys, E.robusta, E.haemastoma)of Eucalyptus in two cultivating place(Yokohama city and Kagoshima prefecture), 2. It compared as the palatability test by the free choice method using three koalas(Phascolarctos cinereus). Koalas(Queensland subspecies:p.c.adustus)were management of Kanazawa Zoo which was established in the Yokohama city. As for the ingredients(dry matter, organic matter, crude protein, gross energy, neutral detergent fiber)of the leaf, the difference was significant among the eucalyptus species. There were more crude protein content(4.6%)of the fresh foliage in Yokohama city than it of Kagoshima prefecture(3.6%). Feed intake was influenced by quantity of provided foliage. Relationship with the specific ingredient wasn't admitted by the palatability, and few changes occurred in the chemical composition. The requirement with quantity nitrogen and gross energy was being satisfied.

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In recent years, getting to have an interest in the preservation of the animal species and the ability of the wild animal with the concern of the human being and the natural environment. Also, in case of breeding of a zoo-animals, the diet expense load was in the rising situation and the rational diet supply standard became found. Therefore, it is in the present situation that the load having to do with expenses is very big. A lot of points which are examined together with the existence or non-existence of the possibility of the substitution diet supply for this purpose are left. It measured 6 ingredients, and NDF, ADF, ADL about the fresh leaf with 8 kinds in amount, 14 kinds of Eucalyptus. In this experiment, it used three male Koalas who are bred in Kanazawa Zoo which was constructed in the Yokohama city. It prescribed chromic oxide for the 1 st as sign material with Milk and Syrup and it measured diet passage speed in the enteron in Koala. With the total collection method, it measured the digestibility of the ingredient of the general composition and the detergent fiber and it made the value of TDN, DE clear to the 2 nd. As for the diet passage speed in the enteron of Koala in the Eucalyptus supply period, all indicator(Cr₂O₃)was collected within approximately 13 days. Then, Koala totaled the feed composition which did voluntary intake every 7 days, but a big change wasn't admitted. The digestibility of Eucalyptus is Dry matters 67.35%, the Organic matter 68.07%, TDN 65.36%, 5.33% of DCP, DE12.34KJ/g, NDF 55.60%, ADF 43.74%, ADL 22.41%, Hemicellulos 64.82%, Cellulose 57.50%.

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This paper describes the relationship between body length and sexual maturity, and the annual reproductive cycle of the male mongoose, Herpestes javanicus auropunctatus, on the island of Okinawa. The relationship between the development of head and body length(HBL) and the state of male reproductive glands, demonstrated them as following;1)that mongooses whose HBLs were under 270 mm were sexual immature animals, 2)that puberty began at HBLs of 270 mm to 289 mm, 3)that the all animals whose HBLs were 320 mm and over had attained puberty. Furthermore, it was strongly indicated that sexual maturity began at HBLs of 290 mm to 319 mm, and that all the animals whose HBLs were 330 mm and over were sexually mature mongooses. The reproductive pattern in the male definitely showed a seasonal cycle, demonstrating the possibility that active reproductive season in the male mongoose is from February to August. However, in inactive season, it is probable that the reproductive activity does not completely fade in a small number of the male mongooses, because the epididymal ducts were visibly present in about 35 % of the sexual mature animals.

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In this study we observed the structure of the anal organs and analyzed the volatile fatty acids of anal sac secretions of the Small Asian mongoose in order to capture the mongoose effectively. The ducts of the animals' anal sac were found to open at the cutaneous zone of the anal canal. The paired anal pockets, with a diameter of approximately 5 mm, a mean weight of 10 mg/100 g body weight, lay at each side of the anal canal. Acinar holocrine glands, which are sebaceous glands, were abundant around the anal sac;in contrast, the tubular apocrine glands were rarely observed. The volatile fatty acids(acetic, propionic, isobutyric, butyric, isovaleric, and valeric acids)were found in the contents of the anal pockets by gas-liquid chromatography. Based on the relative concentrations of these, it was clear that different males have different volatile fatty acid profiles, whereas females have higher concentrations of acetic acid and lower concentrations of isobutyric and valeric acid. Although these volatile fatty acids are not species-specific, some unknown peaks were detected on the chromatogram.

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The survey of the parasitic helminths has been carried out on the pet animals belonging to orders Marsupialia(Philandar opossum, Trichosurus vulpecula, Dactylopsila trivirgata and Macropus eugenii), Edentata(Tamandua tetradactyla and Euphractus sexcinctus), Rodentia(Spermophilus erythrogenys and S.spilosoma)and Lagomorpha(Ochotona sp.). One acanthocephala, Giganthorhynchus sp.from T.tetradactyla, and 10 nematodes, viz., Filarinema sp.from T.vulpecula, Mackerrastrongylidae gen.sp.and Paraustrostrongylus sp.from D.trivirgata, Delicata sp.from T.tetradactyla, Aspidodera sp., Maciela sp.and Moennigia sp.from E.sexcinctus, larval nematodes Nos.1 and 2 from E.sexcinctus and S.spilosoma, and Murielus sp.from Ochotona sp., were obtained. These are the first records of these helminth species from the pet animals transported into Japan.

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For the sake of an epidemiological analysis on the helminth infection of pet tortoises sold in Sapporo, Japan, 57 individuals belonging to 5 families, 21 genera, and 29 species and subspecies, were examined helminthologically. Among them, Neoechinorhynchus sp.(host: Trachemys scripta subsp.), Serpinema trispinosus(hosts: Graptemys geographica, T.scripta subsp., Chelydra serpentina osceola), S.microcephalus(hosts: Siebenrockiella creassicollis, Ocadia sinensis), Atractis cf.dactyluris(Rhinoclemmys pulcherrima subsp., R.p.rogerbarbouri), Cissophyllus sp.(hosts: Graptemys barbouri, Sacalia bealei), and unknown nematodes, were obtained. The brief zoogeographical discussion of the genus Serpinema was given.

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