In this study, the relation of estrogen to the growth of a follicle or ovulation was investigated from the correlation with an egg-laying state, the concentration of a yolk precursor in blood (based on a serum vitellin value), the growth mode of a follicle, the structure of an intraovular yolk sphere or the like by administering antiestrogen (tamoxifen, TAM) or synthetic estrogen (diethystilbestrol, DES) to a laying hen. The results obtained were as follows.
As result of once administering 20mg of TAM to the laying hen orally, the lowering of the serum vitellin values and the growth suppression of the follicle were observed over several days after adiministration. Further, small yellow yolk spheres or yellow yolk spheres of a transfer type from white yolk spheres to yellow ones are distributed at the growth supression region of the follicle and the structure of them was clearly different from that of yellow yolk spheres before administration.
DES was administered to the laying hen in ratios of 15, 30 and 60mg/a hen by intramuscular injection but no large change was confirmed in an egg-laying state in all of the administration groups. On the other hand, the serum vitellin value suddenly rose as the administration amount increased and the lasting period thereof also became long. However, the effect of the administration of DES on the growth of the follicle or the structure of intraovular yolk spheres was not confirmed at all.
From these results, it was cleared that exogenous estrogen DES itself or the serum vitellin value increased by DES exerted almost no effect on the growth of the follicle or ovulation. The growth of the follicle in a rapid growth phase is mainly prescribed by the yolk forming capacity of an egg (oocyte) and, usually, it is considered that the follicle alreay takes in the yolk precursor in the amount corresponding to the saturated amount at the growth stage thereof. However, it was cleared that a change was generated in the growth mode of the follicle or the structure of yolk spheres in the follicle when the marked reduction in the supply amount of the yolk precursor to the follicle and the change in the properties of the yolk precursor were generated.
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