Since the transparent fluid which mixes with the semen from the vas deferens contains blood serum components, the present experiments were performed to find out the effect of addition of blood serum at various levels to the semen dilutents on the spermatozoal motility and metabolism.
Pooled semen from 15 roosters was centrifuged at 3000 r.p.m. for 10 minutes. The precipitate was washed twice with phosphate buffer (Δ=-0.60°C, pH=7.0) containing 3.6mg/ml glucose and resuspended in the phosphate buffer. The spermatozoal suspension was mixed with the phosphate buffer containing various levels of blood serum and the mixture was incubated at 37°C for 1-3hrs. During the incubation, the mixture was shaken. Motility was scored from 0 to 5. Oxygen consumption was measured polarographically with a Clark oxygen electrode.
The results obtained are as follows.
1. Spermatozoa in the medium containing 35 to 75% blood serum showed more vigorous motility at the beginning of incubation and maintained better motility for 3 hours than those in the medium containing 0 to 15% blood serum.
2. Immediately after incubation, average oxygen consumption rate at a concentration of 0, 5, 15, 35, 55 and 75% serum was 3.8, 6.0, 8.9, 13.4, 13.5 and 14.7μl/10¹⁰sperm/min. respectively. The higher concentrations of the serum (35-75%) resulted in more sharp decrease of oxygen consumption rate than the low (0-15%) with the progress of incubation period.
3. During one hour incubation, 35, 55 and 75% serum markedly stimulated glucose utilization, as compared to 0%/ serum. After more than two hours incubation, glucose utilization at a concentration of 35% serum was apparently greater than that at the other concentrations of the serum.
The results suggest that cock blood serum components are closely related to the spermatozoal motility and metabolism.

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Rabbit antisera were prepared against saline extracts of laying hen ovary from which follicles above 1mm in diameter had been removed. The Oucterlony double gel diffusion technique and the immunoelectrophoretic analysis were employed to detect the ovary specific antigens. The unabsorbed antisera developed the following numbers of precipitin bands: anti-ovary versus laying hen ovary, 7 bands; anti-ovary versus other tissue extracts or sera, 4 to 6 bands; anti-ovary versus mammalian ovary, 0 band.
Absorption of the antisera with cock serum, laying hen serum and spleen was necessary to detect antibodies directed against the ovary specific antigen. The following numbers of precipitin bands were observed when antisera absorbed with cock serum were allowed to react against the tissue extracts or sera: anti-ovary versus ovary, 4 bands; anti-ovary versus other tissue extracts and laying hen serum, 1 or 2 bands. The following number of precipitin bands were observed when antisera absorbed with laying hen serum were allowed to react against the tissue extracts or sera: anti-ovary versus ovary, 2 bands; anti-ovary versus liver, spleen and lung, 1 band. One of the 2 bands seen in ovary formed a reaction of identity with liver, spleen and lung.
The antiserum absorbed with cock serum and spleen developed 1 band against ovary only. These results was interpreted as indicating that the ovary contained a tissue-specific antigen. Immunoelectrophoresis revealed that the mobility of this ovary tissue-specific antigen almost corresponded to that of β-globbulin.

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Many reports have been published on the restricted feeding of broiler breeding stock. Is it necessary to always keep layers full diet? The lower feed conversion ratio may bring the more economical profits by means of feed restriction.
This report deals with the effect of feed restriction on the performance of layers and the relationship between the breeds of feed restriction and the economical profit was discussed.
The experiment was carried out with strain cross of White Leghorn (No. 1), two crossbreds of Rhode Island Red _??_ _??_ x White Leghorn _??_ _??_ (No.2, 3) and strain cross of Rhode Island Red (No. 4) hatched on May 14, 1968. Numbers of layers used in this experiment were 162 of the two breeds and two crossbreds. The layers were full diet until 154 days of age. Layers were divided into control group and restricted group at 155 days of age.
Feed restriction was terminated at 519 days of age, and feed restriction rate was 90% of full diet of the control layers. Still more their layers were carried out force molting on account of investigation of effect of feed restriction on performance of two years of age. Their layers were fasted for 7 days from 520 to 526 days of age. Experimental period was for 365 days from 562 to 926 days age. In the both groups no feed restriction was carried out.
Feed consumption of the restricted groups was 88.4% of the control groups during the experimental period. Hen-day egg production of restricted groups was 96.7% of the control group. Samely, the ratio of average egg weignt and egg production per bird per day were 99.3%, and 96.0% of the control groups, respectively. These results were inferior a little to the control. However, the feed conversion ratio of restricted groups was improved 0.24 by feed restriction, therefore it was possible to saving of feed. Body weignt of the restricted groups was 88.6% of the control groups at 500 days of age. The restricted groups lost their body weight by the same ratio of feed intake. Grude profit of restriction in the restrieted groups was much more than that in the control group by 107 yen and that in No. 2, 3, and 4 was much more than that in the No. 1.
We compared with the egg production of both groups after force molting at 520 days of age. It was found that no effect by the treatment of feed restriction.

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[in Japanese]

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[in Japanese]

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