In a multi-center placebo controlled cross over trial, We compared the efficacy of continuous n-3 PUFA enriched food “Ipaole” supplementation (daily intake dose; ALA: 60mg/kg, EPA: 30mg/kg, DHA: 18mg/kg) for atopic dermatitis to that of a isonergic placebo containing olive oil for 4 weeks.
A total of 37 patients with atopic dermatitis, aged 12.8+4.9 years, from 6 investigative centers (male: 17, female: 20; severe: 22, moderate: 15) were employed.
The patients were randomly divided into two groups: A group consists of 20 patients receiving Ipaole preceding placebo; B group 17 patients receiving placebo preceding Ipaole.
Of the 37 patients who started the trial 2 from A group and 2 from B group dropped out.
During Ipaole supplementation a statistically significant improvement was observed in the overall severity and grade of inflammation, and in the percentage of the body surface involved by ezema (p<0.01).
But during placebo administration no significant reduction of symptom score was shown. During Ipaole supplementation the treatment score also significantly decreased (p<0.1) but does not significantly reduced during placebo intake.
During Ipaole supplementation a statistically significant rise of EPA, EPA/AA and DHA of serum phospholipids was observed (p<0.001, p<0.01), but not observed during placebo intake.
Bleeding time prolonged slightly in some patients, but any clinical sign of bleeding was not observed.
These results suggest that the supplementation of n-3 PUFA enriched food is useful in the treatment of some patients with atopic dermatitis.

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From questionnaire data obtained from 5412 general pediatric outpatients, the following observations about adverse drug reaction and drug-hypersensitivity were made: (1) About 9.46% of participating children had experienced adverse drug reactions in the past and 3.75% had experienced symptoms of drug-hypersensitivity. Allergic subjects has a higher prevalence of drug-hypersensitivity. (2) Symptoms of drug-hypersensitivity occurred even in young infants. (3) In drug-hypersensitive children, antibiotics were the most frequent cause. (4) Most parents of drug-hypersensitive children didn't know names of drugs resonsible for drug hypersensitivity symptoms in their children, hadn't obtained an accurate diagnosis of drug allergy or drug eruption from their doctors and were unaware of appropriate drug-hypersensitivity prevention.

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Two cases with intractable bronchial athma were treated with extensive inhalation therapy. The inhalation constituted with sodium cromoglycate+beta2-stimulant+prednisolon was scheduled for 5-6 times a day extended to mid-night and early morning using specially designed inhalation equipment. This protocol of therapy was found to be effective for intractable asthmatic children to induce remissive state.
Case 1: A 15 year-old boy who was diagnosed as bronchial asthma on 1 year old was admitted to our Children's Allergy Center when he was 10 year-old. He had previously experienced frequent hospitalization due to recurrent attacks of asthma, and treated intensively including steroids. After admission the extensive inhalation protocol was initiated, and within 1-2 months asthmatic attacks were almostly relieved.
Case 2: A 15 year-old boy with intractable asthma for 13 and half years was treated with the same regimen. In a few weeks with the extensive inhalation therapy remissive state of asthmatic attacks was achieved.
Thus, the extensive inhalation therapy was revealed effective for both patients with long-term intractable asthma.

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We utilize APR score (CRP, α1-acid glycoprotein [α1-AG], Haptoglobin [Hp]) to diagnose early neonatal infection and comprehend clinical course. We studied the relationship between α1-AG and Hp and the severity of asthma, wheezing, cough, etc.. Subjects were 91 children with asthma who visited our allergic clinic (July 1989-August 1990) and all underwent a blood exam. Marks for severity of wheezing and cough were calculated from an asthma diary. The value for Hp of a child with severe asthma was significantly lower than for one with mild asthma (P<0.05). There was a significant relationship between marks for severity of wheezing and cough and α1-AG on the 3rd day before the blood exam (P<0.05). For subjects greater than 7 years old, however, the value for Hp did not significantly decrease along with a increase in the severity of asthma (mild 120±118.5, moderate 105.5±102.6, severe 69.9±58.1mg/dl). There was a significant relationship between marks for wheezing and cough and α1-AG on the 2nd and 3rd days preceding the blood exam (P<0.05). It is considered that the values for Hp, which decreased in young children with severe asthma, suggest indirectly the value of Hp in the control of asthma

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In recent studies, human basophils that were primed with interleukin-3 (IL-3) or human leukemic basophils were reported to produce IL-4. We had reported that human cord blood derived basophils cultured in the presence of IL-3 for 5 weeks were functionally and morphologicaly mature. We here report the production of IL-4 from cultured basophils, comparing with histamine release and leukotrienes production, following stimulation with anti-IgE antibody. IL-4 production was 107.2±63.2pg/10⁶ basophils at 12800 times diluted anti-IgE whereas maximum histamine release and maximum leukotriene production were 41.4±4.5% and 8.3±3.4ng/10⁶ basophils at 1600 times diluted anti-IgE antibody respectively. Interestingly an optimal concentration to induce IL-4 production was about 10 times lower than that to induce maximum histamine release and leukotrienes production. Kinetics of IL-4 production from the cultured basophils reached a plateau at 6 hours, which was much slower reaction than histamine or leukotrienes production from the cells. We conclude that cord blood derived cultured human basophils produce IL-4 in response to anti-IgE antibody and that the cultured human basophils are functionally mature and useful tool with which to investigate mechanisms of allergic reaction.

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We examined IgG subclass of serum from 182 allergic children usig ELISA method. We defined low level below twofold of standard deviation. Twenty-five children (13.7%) showed low level in more than one IgG subclass. Eighty-eight percent of these children showed normal level of total IgG level. Twenty six point three percent of children with low IgA level showed low level in more than one IgG subclass.
There was no significance between levels of IgG subclass snd IgE. We compared level of IgG subclass and severity of atopic dermatitis and bronchial asthma. There was no correration with severity of atopic dermatitis. However very high population of severepatient with bronchial asthma ahowed lower level in more than one IgG subclass.

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An 8 years old boy who had had chronic urticaria since 4 years old developed severe erythema, edema and tenderness in the whole of right upper extreme after Japanese B encephalitis vaccination. Drug induced lymphocyte stimulating test showed doubtfull for Japanese B encephalitis vaccine of same Lot. Therefore, it is suspected that his severe dermatical reaction was the type IV allergy caused by the Japanese B encephalitis vaccine.

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