Previous research has demonstrated that sanction systems increase cooperation in social dilemma situations. However, specific literature indicates that sanctions undermine cooperation and trust in others. This study defined the detrimental effects of sanctions as decreasing cooperation after sanctions are removed in groups adopting sanctioning compared to a control group playing the public goods game without a punishment system. This study conducted a conceptual follow-up of previous studies and examined whether the detrimental impact of sanctions was consistent. The hypotheses, sample sizes, and analytical design of the study were pre-registered. This study’s result did not conceptually replicate the detrimental effects of sanctions. This finding suggests that the detrimental effects of sanctions, if any, were minimal in the way punishments were introduced in this study.