I detrmined the blood sugar, blood pyruvic acid and blood lactic acid when injected intravenously into dogs in doss of 50 to 150 mg per kilogram. And I observed the changes in the amount of blood sugar, pyruvic and lactic acid when the alloxan diabetes is produced after various previous treatments. And I observed the changes of blood. sugar, pyruvic and lactic acid in normal dogs, alloxan diabetic dogs and pancreatectomized dogs after administration of insulin, epinephrine, glucose, pilocarpin, etc., under the attack of fever and in high temperature.
1) The optimum diabetogenic dose is about 70mg per kilogram of alloxan. The initial hyperglycemia and hypoglycemia following the injection of alloxan in dogs are slower in their appearance and of smaller magnitude than in rabbits. Blood pyruvic and lactic acid remarkably increased within one to five hours after the injection and within seven to thirteen hours after it.
2) Hyperglycemia by oral administration of glucose increases the resistance to the diabetogenic action of alloxan, but hypoglycemia by administration of insulin and hyperglycemia by administration of epinephrine decrease the resistance.
3) The initial hyperglycemia and especially hypoglycemia receive the central influence.
4) The initial hyperglycemia and hypoglycemia are the central action through the liver.
5) In the dogs after the extraction of their salivary glands, the initial hyperglycemia is remarkable, hypoglycemia slight and short, and the permanent hyperglycemia also remarkable. And their surviving times are shorter, and they are less resistant for alloxan diabetes than the normal dogs.
6) When I injected in doses of 70mg per kilogram after the extraction of the left suprarenal gland, blood sugar did not change so remarkably. I believe that the suprarenal gland has an important influence on the changes of blood sugar, pyruvic and lactic acid in alloxan diabetes.
7) Blood sugar increased rapidly after the pancreatectomy, reached to ca. 300mg/per dl. at fourteen hours after the pancreatectomy and since then increased slightly and slowly. Blood pyruvic and lactic acid do not change so remarkably.

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