I have tried the peritoneoscopic cholangiography to 27 patients suffered from the biliary tract disease which defy the usual means of diagnosis.
I punctured directly the fundus of gall-bladder under direct vision, and injected 10, 30 mi. of 35% pyraceton (idopyracet) containing 2ml.(0.5gr) streptomycin solusion. the diameter of needle is 0.9 m. m.
In 14 cases the procedure was safely performed, and the satisfactory rentogenograms were obtained as above seen.(Stone of gall-bladder, or of common duct. Cancer of pankreas, or of papilla Vateri.)
But in two cases such as the pancreas cancer which showed the hydrops of gall-bladder with the outstanding enlargement of liver and the chronic cholecystitis with the biliary dyskinesis, a large amount of bile was escaped throngh the needle hole, and the bile peritonitis was occured.
I can no longer recommend the clinical use of needling gall-bladder wall, since the perfect security of this method can not be obtained.
I believe that the puncturing of gall-bladder from the liver surface, now prevailing in Germany (H. Kalk), is more reliable in spite of its technical difficulties.

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In the I report the existence of anemic substances in the culture medium of alive dog, hook worm was confirmed.
The present study is concerned to the mechanism of the development of anemia by such filtrates of the culture mediums.

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