Though many studies on the cultivation of encysted larvae of hookworms, have. been done, no sure aseptic artificial cultivation method is reported. The author invented new method to cultivate them aseptic in an artificial medium as follows.
Feces of dogs, which are severely infected with hookworms, were mixed with a saturated NaCl solution and filtrated with a wire netting and gauzes to remove impuntes as ingested mattere. By keeping still, the contained eggs floated on the surface of the filtrated solution. Then the eggs wer transfered with a deckglass for a microscopic examination into the water kept in a No.4 glassfilter for the chemical analysis. Atter the Water was filtrated off through the glassfilter a 10% formalin solution was poured in the glassfilter, which contained the eggs, and kept two hours. As it was determ ineil by preliminaly examination that in formalin solutions more than 4% concentration all microben were damaged, but eggs were kept alive in less than 10% solution, the eggs soaked in 10% formalin solion were surely sterilized. Then the eggs were washed a sterilized water several times to remove the formalin.
Thus aseptic eggs were obtained and offered for following exa, minations,
1) The aseptic eggs were cultivated in an, with an autoclave sterilized solution of feces or 1% pepton solution in a glassfilter and hatched out in the next day to the stage of I stadium. They remained unchanged even after 4 days.
2) But when the eggs were cultivated in the above mentiond mediums, to which sponges were added, or cultivated by KAWARA (Japanese tile) culture. method with above mentioned mediums mixed with chacoal at 37°C, larvae, developed, to the stage of II stadium.
3) The eggs were cultivated in the feces charcoal medium in a glass plate, which was sterilized in an autoclave, at 37°C 4 pays and then a 1% sterilized agar was poured over When, after the agar coagulated, 43°C warm sterile water was poured over, it was determined that numerous aseptic encysted larvae in II stadium creeped out in the warm water.
Thus the author achieved to cultivate the encysted larvae of hookworm aseptic.

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