In order to trace the changes in the activity of ions in dipolar aprotic solvents, poly(acrylamide) membrane based ion selective electrodes were developed in the author's laboratory. This review covers (1) the development of the electrodes, including the synthesis of host compound modified poly(acrylamide); (2) potentiometric response properties of the electrodes; (3) investigations of the response mechanism by means of spectrophotometric and cyclic voltammetric methods; and (4) applications to determine the solubility products of slightly soluble electrolytes in dipolar aprotic solvents, successive complex formation constants of ions with some dipolar aprotic solvent molecules, and standard molar Gibbs energies of ions transfer.

抄録全体を表示

An electrochemical on-off regulation of in vitro bacterial luciferase reaction has been reviewed in light of the application to studies on bacterial bioluminescence. The redox process of FMN/FMNH₂ at an electrode is successfully utilized to initiate the bacterial luciferase reaction and to halt it. The electrochemical on-off regulation is versatile to develop not only luciferase turnover but also its single turnover. From the characterization of luciferase reaction based on this method, the thermodynamic behavior for the formation of emitting species has been explained. This method is also beneficial to the characterization of endogenous fluorescent proteins from the point of view of their molecular function in bioluminescence production.

抄録全体を表示

More than 2, 000 papers have been published concerning adsorption of organic compounds on mercury electrodes. Many review articles have been published on the adsorption of organic compounds on electrodes citing these papers. However, there have been not so many articles which make much of adsorbable compounds themselves. The next two monographs are considered to make much of the compounds. The monograph by Breyer and Bauer (Breyer and Bauer, 1963) contains the chapters of a.c. polarography of organic compounds and tensammetric investigation of non-reducible substances. About 1, 100 papers are cited in Jehring's monograph (Jehring, 1974). It contains a detailed index of organic compounds. The author will try to make a dictionary of the compounds which are adsorbed on mercury electrodes. The present review article deals with alcohols. The form of description will follow that of Breyer and Bauer.

抄録全体を表示