-
Kotaro Umeki, Tadashi Imai, Masahiko Goshi, Tomokazu Kojima, Nobuhiko ...
2008 Volume 56 Issue 4 Pages
469-478
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
The purpose of this study is to shed some light on the appropriate sizes of both food organisms and artificial food granules that can best be fed to the larval and juvenile stages of ayu
Plecoglossus altivelis altivelis, taking the relationship to the size of its feeding organs into account. Ayu larvae ranging in standard length (SL) from 6.5 to 15.8 mm were fed two sizes of rotifers
Brachionus plicatilis sp. complex and two sizes of
Artemia sp. nauplii at the same time. The rotifers were sieved out. The widths of the rotifers used for the experiment were 80-120μm and 130-210μm. The width of newly hatched
Artemia was between 100-220μm and that of those one day old was between 100-240μm. Larvae of 7.0-7.9 mm SL fed mainly on small rotifers, and individuals of 8.0-9.9 mm SL ate large rotifers. When the larvae reached 10 mm SL, they shifted their main food to newly hatched
Artemia, and larvae of 14.0-14.9 mm SL fed mainly on one-day-old
Artemia. For the experiments, artificial food granules were sieved out into three different sizes for larvae, and into five different sizes for juveniles. The modes of widths of the sieved artificial food granules for larvae were 120μm, 240μm and 480μm; for juveniles, 300μm, 480μm, 920μm, 1280μm and 1490μm. Ayu larvae ranging from 6.9 to 22.2 mm in SL were fed each of the above three sizes of food granules separately. When the food of 120μm was fed to larvae, food intake increased as they grew from the range between 7.0-12.9 mm to the range between 19.0-21.9 mm SL. When the food of 240μm was fed to larvae, there was no difference in food intake among those that grew from 7.0 mm SL to 18.9 mm SL. Larvae of 19.0-21.9 mm SL, its food intake increased. No larvae fed on the food of 480μm. Ayu juveniles whose size ranged from 16.4 to 58.0 mm in SL were fed each of the above five sizes of food granules separately. Food intake of juveniles with the size ranging from 20.0-24.9 mm SL fed more on the food of 300μm than on the other sizes. Similarly, juveniles whose size ranged from 25.0-34.9 mm SL fed on the food of 300μm and 480μm more than on the other sizes, and those of 35.0-49.9 mm SL ate the food of 480μm more than the other sizes. The ratio of the width of food organisms and food granules eaten and with the mouth sizes of larvae and juveniles was 8.2-37.1%. Thus, the larvae and juveniles of ayu tended to eat food of a relatively smaller size.
View full abstract
-
Yoshihiro Suzuki, Takahiko Suzuki, Yuji Sagishu, Thoru Mekata, Tomoya ...
2008 Volume 56 Issue 4 Pages
479-485
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
Using a closed recirculating system including foam separation, nitrification and denitrification units for kuruma shrimp
Penaeus japonicus, a mating test was carried out in the limited space of the culturing tank (area, 1 m
2). The shrimp used in the test were judged in advance to be free of the white spot disease virus (WSDV) by the method of loop-mediated isothermal amplification (LAMP). The mating behavior was judged by observing the stopper of the female shrimps. All individuals in the system survived throughout the test period (105 days). The foam separation unit maintained oxygen saturation in the water used for rearing at 99%. Ammonia and nitrite oxidation were accomplished rapidly and simultaneously in the nitrification unit. The ammonium ion and nitrite concentrations were kept at less than 0.2 mg-N/
l. Furthermore, contaminants such as suspended solids, chromaticity substances, organic substances and proteins absorbed on the stable foam were removed from the culture water by foam separation. This system was able to maintain good water quality for culturing shrimps under a closed condition without water exchange when shrimps were fed both a commercial diet and frozen lugworm. In such a culturing environment, 40% of the population of female shrimps formed a stopper at the thelycum within 1-2 months after the mating test was started. After the mating test, the shrimp cultured in the system were judged WSDV free by the LAMP method. In the small-scale of closed recirculating system, it was verified that female shrimps possessing a stopper could be produced under virus-free conditions.
View full abstract
-
Ken-ichi Yamamoto, Takeshi Handa, Naoto Kayano
2008 Volume 56 Issue 4 Pages
487-491
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
In the pen-shell,
Atrina (
Servatrina)
lischkeana, change of ventilation with decrease of oxygen pressure by oxygen uptake of the pen shell was examined at 13, 22 and 28°C. Under normoxic condition, ventilation volume and moving speed of a piece of vinyl film put on the gill surface showed the high value, as water temperature was higher. However, expired water volume per stroke was the almost constant and the period of one ventilation cycle showed the shorter value. Under hypoxic condition, the oxygen pressure in which the ventilation pattern changed from the intermittent type to the continuous type showed the high value, as water temperature was higher (47.3 mmHg at 13°C, 59.6 mmHg at 22°Cand 102.3 mmHg at 27°C), though the moving speed was the almost constant. From these results, the pen shell was supposed to regulate the ventilation volume by the control of mantle lobes, without changing the activity of the cilialy movement at the gills.
View full abstract
-
Ken-ichi Yamamoto, Takeshi Handa, Masahiro Kayano, Masashi Siraishi
2008 Volume 56 Issue 4 Pages
493-496
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
Effects of water temperature on coelomic pressure and ventilation in the respiratory tree of the Japanese common sea cucumber,
Aposticopus japonicus were examined using the method which continuously measured the ventilation volume. With increase of water temperature, the ventilation volume per unit time in the respiratory tree increased by increasing the inhalation volume per one inhalation and the inhalation frequency per one ventilation, and decreasing the periods per one inhalation and per one exhalation. And the minimum and maximum of coelomic pressure per one ventilation increased with increasing the inhalation volume per one inhalation.
View full abstract
-
Mahiko Abe, Masahiro Kobayashi, Motoya Tamaki, Eiji Fujiyoshi, Norio K ...
2008 Volume 56 Issue 4 Pages
497-503
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
In order to discriminate between
Porphyra tenera Kjellman and
P. tenera Kjellman var.
tamatsuensis Miura, the partial sequences (670 bp) of mitochondrial DNA related to ATP6 gene were examined for use as a genetic marker. The partial sequences were identical within the three strains examined of
P. tenera var.
tamatsuensis, but the sequences had a single substitution compared with the three strains examined of
P. tenera. The region used in the present study is concluded as an effective marker for discrimination between
P. tenera var.
tamatsuensis and
P. tenera. Moreover, the sequences of two closely related species
P. yezoensis Ueda f.
narawaensis Miura and
P. tenera had differences of 20-21 bases. Thus, this region may also allow discrimination of species within genus
Porphyra.
View full abstract
-
Choon Looi Ch’ng, Shigeharu Senoo
2008 Volume 56 Issue 4 Pages
505-512
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
To establish a seed production technique for a new hybrid, tiger grouper
Epinephelus fuscoguttatus×giant grouper
E. lanceolatus, development of the eggs and larvae were observed. Newly ovulated eggs from a female
E. fuscoguttatus of 8.2 kg in body weight were measured at 0.84±0.03 (mean±SD) mm in diameter and weighed 2,588 eggs/g. After fertilization with sperm from a male
E. lanceolatus, the eggs absorbed water and acquired a spherical shape with a hard covering membrane. Each egg had an oil globule which measured 0.21±0.01 mm in diameter. The hatching started from 18-19 hours after fertilization under 28.5°C and 30 ppt salinity. The fertilization and hatching rates were 86.8% and 87.2%, respectively. The larval development was almost similar to those of the other species. Newly hatched larvae were 2.00±0.30 mm in total length and floated motionless at water surface. Larval mortality was high at early stages and stabilized from 15 days after hatched (d AH). Larvae commenced shifting habitat from pelagic to benthic at 30 d AH. One thousand seven hundred fifty three tails 30 d AH juveniles with mean total length 22.5±2.7 mm were produced from 324,000 hatched larvae.
View full abstract
-
Koji Takeno, Yuichi Hamanaka, Isao Okano
2008 Volume 56 Issue 4 Pages
513-522
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
In order to clarify the growth of young Japanese flounder
Paralichthys olivaceus (ca. 2 months to 15 months post-hatch, size ca. 9 to 40 cm
TL) in semi-enclosed waters, we examined their somatic growth and condition factor in two bays, Kumihama Bay and Aso Bay. Specimens sampled from Kumihama Bay showed lower growth rates and condition factor values compared to those from Aso Bay. The stomach content analysis revealed that most of the flounder preyed extensively on fish throughout the year in Aso Bay, whereas approximately half of the fish sampled in spring and summer showed stomach contents of the flounder had changed their diet from fish to crustaceans in Kumihama Bay. Although there were no significant differences in the mean stomach contents indices used as an index of feeding intensity between the two bays, these indices for fish that fed on crustaceans were significantly lower than those that fed on fish. These results indicate that the inferior growth of young flounder in Kumihama Bay was due to the low availability of small fish prey such as Japanese anchovy and gobies during the high growing season in spring and summer.
View full abstract
-
Nguyen Thanh Binh, Manabu Ishikawa, Saichiro Yokoyama, Fady Raafat M ...
2008 Volume 56 Issue 4 Pages
523-530
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
Wild caught female broodstock kuruma shrimp were unilaterally ablated and treated with 4 different feeding regimes: basal diet (steam pellet), powdered polychaete-basal diet combination, fresh frozen polychaete and no-feeding. Polychaete was dried in freeze dryer, ground into powder and further incorporated into basal diet with the amount corresponding to 345 g/kg. Eight females were placed in each 4 feeding regimes, and animals were fed with pellet diets at 3% biomass and with frozen polychaete at about 10% biomass. Females’ gonad developments were individually recorded every 4-day period for 28 days. The ovary shadow ratios (ovary shadow width×100/body width) and the spawning rates were taken into considerations for examining the effects of dietary treatments on ovarian maturation and spawning performance of kuruma shrimp. The results showed that both treatments of fresh frozen polychaete and polychaete powder-basal diet combination had the highest numbers of spawners and efficacy of stimulating ovarian development, followed by basal diet group during experimental period. No-feeding treatment showed only the regression trend of gonads following the experimental time. Fatty acid compositions of hepatopancreas and ovaries dissected from the experimental females were similar in all treatment groups except that from no-feeding treatment. This preliminary study demonstrated that the formulated diets could induce the maturation and spawning of kuruma shrimp.
View full abstract
-
Shinya Mizuno, Naoyuki Misaka, Tetsuo Teranishi, Daisei Ando, Tatsuya ...
2008 Volume 56 Issue 4 Pages
531-542
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
We examined the physiological effects of an iron citrate (IC) supplement to commercial salmon food on hatchery-reared chum salmon (
Oncorhynchus keta) fry. The fry were divided into four groups, each held in 60-
l acrylic tanks, and fed either commercial salmon crumble (control diet), 0.125% IC enriched (iron-1), 0.25% IC enriched (iron-2) or 0.75% IC enriched (iron-3) test diets for 3 months. Total intake of IC enriched diet for each group was adjusted to 400 g. Dietary IC supplementation had no effect on fry growth, feed efficiency, survival rate, hematological parameters, seawater tolerance, whole body RNA/DNA, total protein content and acid phosphatase activity. On the other hand, burst swimming velocity, tolerance to fasting, gill Na
+, K
+-ATPase activity, cavity space in the liver, whole body triglyceride, ATP content, acid protease, cytochrome oxidase and citrate synthase activities and transcript levels of cytochrome
b, all were increased significantly by IC supplementation. These effects were more pronounced in fry fed with iron-3 diet. The results reveal that dietary IC supplementation to the commercial salmon crumble is effective in producing high-quality chum salmon fry.
View full abstract
-
Kenjiro Yoneyama, Gunzo Kawamura, Yoshimi Horikiri
2008 Volume 56 Issue 4 Pages
543-549
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
Naive rainbow trout were injected with total RNA extracted from the brains of conspecifics which have learned to avoid fishing hook through a capture-recapture angling process. This treatment was applied to 6 test groups. Each group was then subjected to a line fishing together with naive specimens injected with physiological salt solution (control 1) or without any treatment (control 2). The mean quantity of total RNA extract varied from 0.06 to 0.33 mg per 1.0 g of brain tissue for each test group. The catchability was much lower in the total RNA injected fish than in controls in four of six fishing trials. The effect of injecting total RNA from the brains of learned specimens on naive specimens was evident. The catchability in the controls was lower than in fish used in the capture-recapture process, indicating the transfer of hook avoidance behavior of total RNA injected fish to naive fish.
View full abstract
-
Kenrou Kurohara, Yoshihiro Kimura, Yousuke Sekiguchi, Kenji Kawai
2008 Volume 56 Issue 4 Pages
551-558
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
To investigate the distribution and proliferation of
Edwardsiella tarda in tissues, Red sea bream
Pagrus major (BW: 43.3-121.7g) were infected by intraperitoneal injection (IP) and immersion infection (IM). Three fish were removed at 0, 1/24, 1, 2, 4, 8 and 12 days after infection, and the blood, liver, spleen and intestine were employed for viable
E. tarda cell count by agar plate method. Viable cell count in the spleen was performed on both live and dead fish, and the count in intestinal contents after IM was also determined. The general profile of bacterial count in the blood, liver and spleen after infection was similar for both methods showing the highest count at after 4-6 days. However, higher max value in the bacterial count and tubercle like white spot in the spleen specific to edwardsiellosis were demonstrated remarkably by IM. Different profiles in bacterial count in the tissue of intestine were observed between two methods. 10
8.34-10
8.45 CFU/g at 1/24 days after IM, decreased to under detection limit then increased to 10
6.13-10
8.45 CFU/g again. This change was not influenced by the bacterial count of intestinal contents. In the IM, cumulative mortality increased continuously, and the bacterial counts in the spleen were similar between live and death fish after 3 days. These results suggest that IM is more adequate experimental infection method.
View full abstract
-
Heisuke Oono, Kishio Hatai, Hideaki Aikawa, Hideo Hara, Masayuki Miura ...
2008 Volume 56 Issue 4 Pages
559-565
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
2-Methyl-4-isothiazolin-3-one (MT) was investigated for toxicity to fish and its efficacy to control fungal infections, compared with bronopol (BP) that is commonly used in hatcheries as an anti-fungal agent. Toxicity of MT and BP for ayu
Plecoglossus altivelis and rainbow trout
Oncorhynchus mykiss fingerlings determined by the 50% lethal concentration (LC
50) and 30 min-treatment for successive days showed that MT was higher than BP in both fishes. Repeated exposure of eggs just after fertilization to the eye development stage with BP and MT at 50 and 100 ppm showed an efficacy compared to the 0 ppm treatment (control) for inhibition of fungal infections in ayu eggs and rainbow trout eggs.
View full abstract
-
Mahiko Abe, Akira Kurashima, Miyuki Maegawa
2008 Volume 56 Issue 4 Pages
567-572
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
Annual and daily net production of
Zostera marina population in Matsunase, Ise Bay, Mie prefecture were estimated by using Biomass method from Sep. 2000 to Sep. 2001 considered with leaf life span and plastochrone interval. In dry weight basic, annual net production was 1332.8 g/m
2/year (above-ground organs; 960.7 g/m
2/year, below-ground organs; 372.1 g/m
2/year). The maximum daily net production of above-ground was 8.0 g/m
2/day, that of below-ground was 2.7 g/m
2/day, and that of total was 8.7 g/m
2/day, respectively. Biomass method was slightly used for
Z. marina population until now, while this method can effectively contribute to accumulate basic knowledge relating the production of
Z. marina in the future.
View full abstract
-
Ryota Miyamoto, Naoyuki Suguro, Kosuke Takaku, Kazumi Hosoya
2008 Volume 56 Issue 4 Pages
573-579
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
The most suitable food set for the larvae and juveniles of an endangered fish
Hemigrammocypris rasborella was investigated by rearing experiments to specify one of optimal preservation techniques. Larvae collected by artificial propagation using HCG, were stocked into 8 tanks at a density of 50 individuals per tank. In the rearing experiment, four different sets of foods were tested : Sec. I, S-type rotifers (SR) -
Artemia nauplii (AN)- Artificial Formula Feed (AFF); Sec. II, SR - AFF; Sec. III, AN - AFF; Sec. IV, AFF solely. The larvae and juveniles of
H. rasborella showed a high survival rate whenever the food set included SR. They grew faster in Sec.III fed with AN. However, their survival rate was lower than that when fed with SR. Single feeding with AFF, always showed the lowest survival rate and poorest growth rate. These findings suggest that the larvae and juveniles of
H. rasborella absolutely need live food for growth and development.
View full abstract
-
Sompoth Weerakhun, Kishio Hatai
2008 Volume 56 Issue 4 Pages
581-586
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
Mycobacterium marinum isolated from yellowtail and amberjack grew slowly between 15 and 32°C, with an optimal temperature of 25°C. All
M. marinum isolates were positive for catalase. They did not produce acids from any carbohydrates tested and were negative for the utilization of 11 carbon sources. The characteristics of drug resistance of
M. marinum were variable. They were sensitive to hydroxylamine hydrazide (0.5 mg/
ml), isoniazid (1 and 10μg/
ml), picric acid (2 mg/
ml) and 5% NaCl. However, they were resistant to T2H at 1μg/
ml and thiacetazone at 10μg/
ml.
M. marinum was not able to decompose or digest tested proteins. Of the water conditions examined, seawater was preferred. They could not survive under conditions of 37°C for more than 1 month but were still viable after about 3 to 6 months under conditions between 5 and 25°C.
View full abstract
-
Tatsuya Yurimoto, Mikihide Yoshida, Yukio Maeno
2008 Volume 56 Issue 4 Pages
587-594
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
Mass mortality of infaunal bivalve, pen shell
Atrina pectinata, occurred in the tidal flat of the northeastern Ariake Bay during early summer in 2004. The characteristic abnormal feature of pen shells was protruding above the sediment surface prior to the death. In these abnormal pen shells, the histological observations revealed serious lesion in the epithelial cells of the digestive gland as well as those of the gill. The nutritional condition of these pen shells was examined compared with those completely buried in the sediment. The contents of chlorophyll
a and phaeo-pigments in the digestive gland as well as glycogen in the adductor muscle of pen shells protruded above the sediment surface were significantly much lower than those of pen shells buried in the sediment. The parameters of feed availability in the adductor muscle and digestive gland, and the histological examination in these organs reflected the deficiency in feeding activity of the pen shells protruded above the sediment surface, strongly suggesting that the mass mortalities of pen shell in Ariake Bay could be caused by continuous starvation.
View full abstract
-
Daisuke Arai, Ayako Kurihara, Ryosuke Komi, Akio Iwamoto, Toshio Takeu ...
2008 Volume 56 Issue 4 Pages
595-600
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
Growth, survival rate, and carcass fatty acid composition of reared common octopus
Octopus vulgaris paralarvae were investigated on different feeding treatments of Pacific sandeel
Ammodytes personatus flakes together with Tibetian Artemia 2 ind. /
ml. Three feeding levels of Sandeel flake, given 4.5, 9.0 and 13.5 g per 4,500 paralarvae/1.5
kl tank, were given three times per day and reared until 25 days after hatching. The paralarvae fed on 4.5 g flake showed lower body weight and mantle length than other treatments. The survivals of the paralarvae fed on 13.5 and 9.0 g flake were higher than those fed on 4.5 g flake. Carcass DHA, total n-3HUFA, and DHA/EPA of the paralarvae fed on 13.5 g flake were higher than other treatments. It is revealed that sandeel over 9 g a time recommended to given on the paralarvae for obtaining suitable growth, survival rate and carcass fatty acid composition under the present rearing condition.
View full abstract
-
Toshio Doi, Ayako Noda, Natsuki Hama
2008 Volume 56 Issue 4 Pages
601-602
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
Histological specimens of the tissue of a red-spotted grouper
Epinephelus akaara infected by
Lernaeenicus ramosus were observed. The muscle of the host was inflammated around the intruding head horn of the parasite, resulting in granuloma. Another host,parasitized by three copepods on the dorsal trunk, recovered spontaneously in captivity over a period of six months without any treatment.
View full abstract
-
Shigeki Dan, Masahiko Koiso
2008 Volume 56 Issue 4 Pages
603-604
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
This study was conducted to examine the effect of microalgal addition on n-3HUFA contents of the rotifer
Brachionus plicatilis in fish mass seed production tank. Three kinds of microalgae were supplemented to 25
kl tanks where rotifers were installed. The n-3HUFA contents of rotifer immediately increased in
Nannochloropsis oculata and n-3HUFA-fortified
Chlorella vulgaris supplemented groups after microalgal addition in experimental tanks, but decreased in
Chlorella vulgaris and no microalgae supplemented groups. It was suggested that fatty acid composition of the rotifer may reflect that of microalgae forms.
View full abstract
-
Masatoshi Ban, Katsuaki Hirasawa, Mutsuko Ezure
2008 Volume 56 Issue 4 Pages
605-606
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
In order to examine the effects of growth on sexual maturation in sockeye salmon,
Oncorhynchus nerka, smolts were marked with PIT tags and reared in an aquarium filled with seawater from May (age-1) to October (age-2). Their fork lengths were individually tracked every four or five months throughout the experiment. At the end of the experiment, 10 females and 13 males matured while 7 females and 3 males remained immature. Distinct differences in the fork lengths of mature and immature fish were observed from August and December at age-1. In particular, the immature group showed extremely low growth during this period. This result suggests that low autumn growth can inhibit the onset of sexual maturation in this species.
View full abstract
-
Takanobu Goto, Yuta Endou, Yuichi Kitaoka, Fumihiko Hasumi, Shin-Kwon ...
2008 Volume 56 Issue 4 Pages
607-608
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
In order to investigate the biosynthesis of taurine in common carp, organic distributions of cysteinesulfinate decarboxylase (CSD) and cysteamine dioxygenase (CAO) activities were determined. The highest CSD activity was observed in kidney, followed by brain, hepatopancreas and gill. On the contrary, the highest CAO activity was observed in hepatopancreas, which value was twice as high as hepatopancreatic CSD activity. A weak activity was detected in intestine, spleen, heart and brain.
View full abstract
-
Shigeru Aoyama, Aki Sato
2008 Volume 56 Issue 4 Pages
609-610
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
The spawning behavior of the Hokkaido eight-barbel loach,
Lefua nikkonis, an endangered species of the family Balitoridae in Japan, were observed in a pair reared in an experimental tank. Spawning occurred in January 2007 with a water temperature of ca. 18°C which had declined from ca. 28°C in summer. On days when spawning occurred, the male chased the female and pecked at the female. Then they burrowed into the artificial spawning substratum and spawned remaining adjacent to each other. The pair spawned 7 batches of eggs during the 21 day reproductive period; therefore batch-spawning was confirmed in this species.
View full abstract
-
Atsushi Yamamoto, Takanori Maegawa
2008 Volume 56 Issue 4 Pages
611-612
Published: December 20, 2008
Released on J-STAGE: September 15, 2012
JOURNAL
FREE ACCESS
We tried to find useful bacteriophages against
Edwardsiella tarda, a causative agent of edwardsiellosis from water samples of eel farms in order to control the disease in eel. The useful phages for phage typing of
E. tarda could not be found because 175 strains of
E. tarda used in this study were divided into 15 phage types by 8 strains of
E. tarda phages and 87 strains of
E. tarda were not sensitive to the phages. Strict host specificity of bacteriophage suggested the wide variety of
E. tarda in eel ponds.
View full abstract
