As a fundamental studies on the seedling of Pacific herring,
Clupea pallasi, the methods of egg-taking and incubation were examined.
Matured brood fish were caught by gill net or set net in bay of Mangoku-ura, Miyagi Prefecture. Ova were stripped by hand massage and inseminated with minced testis. Artificial insemination were carried out 3 times during the spawning season from December 1982 to January 1983.
Fertilized eggs were dispersed on the nest made of saran-net (1 mm streched mesh) with wooden frame. The rate of eyed eggs and hatching were examined under different conditions. The results are summarized as follows :
1) It is important to disperse the eggs uniformly on the nest; recommendable density was 30 to 40 eggs per cm
2.
2) The eggs incubated at 10°C (10.5-12.7°C) or 15°C (13.5-15.0°C) were normally developed and hatched. The rate of eyed eggs was more higher at 20°C (13.5-19.7 °C), while the rate of hatching was higher at 10°C and 15°C. The viability of the larvae was weak at 20°C and a half of them died until 2 to 3 days.
3) Temperature-day degree from fertilization to hatching was given following parabolic expression :
D (days) =278.35T-1.315 (°C) (r=0.995)
4) Temperature-day degree from fertilization to eyed stage was 75 D° at each temperature. After eyed stage, however, the temperature-day degree until hatching was different and varied from 115 to 145 D°.
5) There was a close relationship between the rate of fertilization and the time after death of brood fish. The rate was decreased suddenly when the time passed over 6 hours.
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