In order to know an efficient rate of food supply for cultivation of rotifers,
Chlorella density of the highest food conversion rate was estimated at different temperatures, with several feeding densities.
Five feeding densities, 0.5×10
6, 1×10
6, 3×10
6, 5×10
6, and 8×10
6 cells/m
l, were prepared for the test series. The temperature was adjusted to 20, 25, 30, and 35°C in each series. The rotifers were cultured in a 13-
l transparent plastic tank for 14 days. Marine
Chlorella was fed continuously to the tank by a peristaltic pump in order to maintain a constant density.
Linear regressions with correlation coefficients of r>+0.90 were observed between food density and food consumption rates. On the other hand, logarithmic regressions with correlation coefficients of r>+0.82 were obtained between food density and population growth. The food densities of 1.6×10
6, 1.8×10
6, 2.0×10
6, and 2.2×10
6 cells/m
l were calculated as those with the highest food conversion rates at 20, 25, 30, and 35°C, respectively, estimated by the intercepts of the food consumption rate and population growth regression lines.
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