Seibutsu Butsuri
Online ISSN : 1347-4219
Print ISSN : 0582-4052
ISSN-L : 0582-4052
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Volume 49, Issue 6
Issue 286
Displaying 1-25 of 25 articles from this issue
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Perspective
Overview
  • New Insights into the Mechanism of Protein Folding by Single Molecule Detection
    Kiyoto KAMAGATA, Masahito KINOSHITA, Satoshi TAKAHASHI
    2009 Volume 49 Issue 6 Pages 282-286
    Published: 2009
    Released on J-STAGE: November 26, 2009
    DOIhttps://doi.org/10.2142/biophys.49.282
    JOURNAL FREE ACCESS
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    To understand the molecular mechanism of protein folding, it is necessary to reveal the dynamics of single proteins from the unfolded state to the folding transition state. We therefore developed a new system that can detect fluorescence signals from free single proteins for extended time periods. The system enabled us to characterize a slow conformational dynamics for the unfolded state of cytochrome c. Furthermore, we proposed a method to estimate the equilibration time for the intermediate state of protein folding.
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Topics
Topics—Young Scientist Series
Theoretical and experimental techniques
Activity of Regional Branch
Young Voice
Letters from Abroad
Keywords of Biophysics
Promenade along Protein 3D Structures
Technical term
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