- Issue 6 Pages 295-
- Issue 5 Pages 243-
- Issue 4 Pages 189-
- Issue 3 Pages 133-
- Issue 2 Pages 75-
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Takeshi MURATA2023 Volume 63 Issue 5 Pages 243
Published: 2023
Released on J-STAGE: November 25, 2023
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Kazuya ISHIKAWA, Yutaka KODAMA2023 Volume 63 Issue 5 Pages 247-251
Published: 2023
Released on J-STAGE: November 25, 2023
JOURNAL FREE ACCESS FULL-TEXT HTMLPlant cells regulate their metabolic activity in response to fluctuating environments, in part by changing the arrangement and interaction of organelles. This means that if we can manipulate the interactions of organelles, the plant can be engineered to have desirable metabolic activities. We recently developed the “organelle glue technique”, in which inter-organellar interactions are manipulated by using the multimerization property of fluorescent proteins. Using this technique, we established transformed plants with altered metabolic activity at the pathway level. Here, we review the current state of the organelle glue technique.
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Satoshi YASUDA2023 Volume 63 Issue 5 Pages 252-256
Published: 2023
Released on J-STAGE: November 25, 2023
JOURNAL FREE ACCESS FULL-TEXT HTMLThe thermostabilization of G-protein coupled receptors (GPCRs), which are important drug targets, is a crucial challenge for structural analysis. We constructed a methodology for thermostabilizing a GPCR in the inactive state or the active state solely by multiple amino-acid mutations without the ligand binding. This method combines our recently developed theory based on statistical thermodynamics and the technique of site-directed saturation mutagenesis, which is frequently used in evolutionary molecular engineering. The methodology was illustrated for the serotonin 2A receptor and the adenosine A2A receptor, and we successfully obtained stabilized multiple mutants.
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Keiichi INOUE, Masae KONNO, Yuma KAWASAKI, María del Carmen MARÍN2023 Volume 63 Issue 5 Pages 257-260
Published: 2023
Released on J-STAGE: November 25, 2023
JOURNAL FREE ACCESS FULL-TEXT HTMLSchizorhodopsin (SzR) is a new family of light-driven inward proton-pumping rhodopsins discovered in the genomes of Asgard archaea. The genomic survey revealed the presence of SzR in a wide variety of environments including high-temperature waters. The X-ray crystallographic structure suggested that a convergent evolution occurred at the molecular level between SzR and xenorhodopsin, another inward proton-pumping rhodopsin. Interestingly, outward proton-pumping rhodopsin was converted to an inward proton pump by swapping only three residues in the retinal Schiff base region with corresponding ones of SzR, indicating the direction of proton transport is determined by this core region of rhodopsins.
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Yohei YAMAGUCHI, Masatoshi MORIMATSU2023 Volume 63 Issue 5 Pages 261-262
Published: 2023
Released on J-STAGE: November 25, 2023
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Inference of Fitness Landscape Based on Multi-Dimensional Phenotype Measurements of Escherichia coliChikara FURUSAWA, Junichiro IWASAWA, Tomoya MAEDA2023 Volume 63 Issue 5 Pages 263-265
Published: 2023
Released on J-STAGE: November 25, 2023
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Yosuke TANAKA2023 Volume 63 Issue 5 Pages 266-269
Published: 2023
Released on J-STAGE: November 25, 2023
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Takuma TOYONAGA, Makoto MIYATA2023 Volume 63 Issue 5 Pages 270-272
Published: 2023
Released on J-STAGE: November 25, 2023
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Yuya NISHIDA, Yasunori SHINTANI2023 Volume 63 Issue 5 Pages 273-275
Published: 2023
Released on J-STAGE: November 25, 2023
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Yuki TOYAMA2023 Volume 63 Issue 5 Pages 276-280
Published: 2023
Released on J-STAGE: November 25, 2023
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Yuji SUGITA2023 Volume 63 Issue 5 Pages 282-284
Published: 2023
Released on J-STAGE: November 25, 2023
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[in Japanese], [in Japanese], [in Japanese]2023 Volume 63 Issue 5 Pages 285-286
Published: 2023
Released on J-STAGE: November 25, 2023
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[in Japanese]2023 Volume 63 Issue 5 Pages 287-288
Published: 2023
Released on J-STAGE: November 25, 2023
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[in Japanese]2023 Volume 63 Issue 5 Pages 289-290
Published: 2023
Released on J-STAGE: November 25, 2023
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2023 Volume 63 Issue 5 Pages 281
Published: 2023
Released on J-STAGE: November 25, 2023
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