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Volume 15 (1975)
- Issue 6 Pages 277-
- Issue 5 Pages 229-
- Issue 4 Pages 153-
- Issue 3 Pages 101-
- Issue 2 Pages 57-
- Issue 1 Pages 1-
Volume 15, Issue 6
Displaying 1-5 of 5 articles from this issue
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[in Japanese], [in Japanese], [in Japanese]1975 Volume 15 Issue 6 Pages 277-288
Published: November 25, 1975
Released on J-STAGE: May 25, 2009
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Hitoshi SAKANO, Yoshiro SHIMURA1975 Volume 15 Issue 6 Pages 289-301
Published: November 25, 1975
Released on J-STAGE: May 25, 2009
JOURNAL FREE ACCESSThe post-transcriptional "maturation" process involved in the biosynthesis of tRNA is reviewed in the light of our recent finding on tRNA precursors and the mode of their enzymatic cleavage. A number of tRNA precursors were identified in a thermosensitive mutant of E. coli defective in RNase P and extensively characterized by fingerprinting and by in vitro cleaving reaction. These studies have revealed the following features of maturation process:
(1) Many tRNA genes, if not all, are clustered in the E. coli genome and thus transcribed in multimeric precursor forms, although the tRNA gene clusters are rather scattered throughout the entire chromosome.
(2) The multimeric precursors are cleaved by RNase O at the spacer regions into monomeric and/or dimeric forms, which are subsequently processed by RNase P at the 5' sides of the molecules into smaller monomeric forms. The monomeric intermediates are then trimmed at the 3' termini by RNase Q.
(3) Modification of nucleotides seems to occur at distinct stages of maturation process, thereby indicating that modification and trimming reactions are somehow interrelated.View full abstractDownload PDF (5048K) -
Yoshinori ISOGAI1975 Volume 15 Issue 6 Pages 302-309
Published: November 25, 1975
Released on J-STAGE: January 07, 2010
JOURNAL FREE ACCESSRecent studies on self-organization mechanism of protein folding are reviewed. First, brief descriptions of the difference between thermodynamical and self-organizational mechanism of protein folding are made. Then it is stressed that self-oranization mechanism is favorable for understanding the quick folding of the tertiary structure of protein and the strong cooperativity of denaturation-renaturation phenomena. Some theoretical and experimental evidneces for self-organization mechanism are critically reviewed.View full abstractDownload PDF (468K) -
Noriyoshi SAKABE, Kiwako SAKABE1975 Volume 15 Issue 6 Pages 310-328
Published: November 25, 1975
Released on J-STAGE: May 25, 2009
JOURNAL FREE ACCESSThis review contains the historical development on the insulin structure study which was begun in 1935 by D.C. Hodgkin, and the relationship between the structure and the biological activity. As 2 Zn insulin crystals belong to space group R3 and the positions of the main heavy atom in the most derivatives are very close to the three fold axis, many difficalties had to be overcome during the structure determination. We describe the three dimensional arrangement of atoms in the molecule in 2 Zn insulin crystal and particularly its states of aggregation. The binding site of insulin receptor is proposed on the basis of the three dimensional structure, biochemical studies, and the studies using modified insulin, and the structure of proinsulin is also mentioned.View full abstractDownload PDF (1915K) -
1975 Volume 15 Issue 6 Pages 336-342
Published: November 25, 1975
Released on J-STAGE: May 25, 2009
JOURNAL FREE ACCESSDownload PDF (1386K)
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