Seibutsu Butsuri
Online ISSN : 1347-4219
Print ISSN : 0582-4052
ISSN-L : 0582-4052
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Volume 54, Issue 5
Issue 315
Displaying 1-16 of 16 articles from this issue
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Perspective
Overview
  • Structure and Function of CRISPR-Cas System
    Akeo SHINKAI
    2014 Volume 54 Issue 5 Pages 247-252
    Published: 2014
    Released on J-STAGE: September 30, 2014
    DOIhttps://doi.org/10.2142/biophys.54.247
    JOURNAL FREE ACCESS
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    Adaptive immunity is acquired when foreign substances enter the body. Interestingly, this immune system exists not only higher organisms but also many bacteria. The CRISPR-Cas systems are the bacterial adaptive immune systems against foreign DNA and RNA derived from phages or plasmids. Many bacteria can memorize the sequences of foreign DNA and RNA, and remove them by means of these systems. Structural and functional studies of these systems have dramatically progressed over the past few years. In particular, one of the CRISPR-Cas systems, Cas9, has received considerable attention as a highly efficient tool for genome editing.
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Review
  • Visualization of Intracellular Temperature Distribution Using A Thermoresponsive Fluorescent Protein
    Shigeki KIYONAKA, Reiko SAKAGUCHI, Yasuo MORI
    2014 Volume 54 Issue 5 Pages 253-256
    Published: 2014
    Released on J-STAGE: September 30, 2014
    DOIhttps://doi.org/10.2142/biophys.54.253
    JOURNAL FREE ACCESS
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    Thermoregulation to conserve body temperature is vital to life in all animals. Multiple mechanisms localized in subcellular organelles have been proposed for thermogenesis. However, direct observation of thermogenesis in intact organelles has not been achieved yet. Recently, we developed genetically-encoded thermosensors ‘tsGFPs’. In tsGFPs, a tandem formation of coiled-coil structures of the Salmonella thermosensing protein, TlpA, transmits conformational changes to the inserted GFP to convert temperature changes into visible and quantifiable fluorescence changes. In mammalian cells, specific targeting of tsGFPs visualized thermogenesis in discrete organelles, notably mitochondria in brown adipocytes and the endoplasmic reticulum in myotubes.
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Topics
Topics (Young Scientist Series)
Theoretical and experimental techniques
Salon
Activity of Regional Branch
Young Voice
Letters from Abroad
Technical term
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