A controlled, comparative open study was conducted on the efficacy and safety of ozone-bubble bathing for seborrhea in dogs. Fourteen dogs were bathed for 10 minutes with 0.015 ppm ozone generated by a bubbling device. Seborrhea, hair shine, hair loss, dandruff, and odor were assessed before, immediately after (T0), and one week after bathing (T7). The Wilcoxon matched-pairs signed-rank tests was used for statistical analysis. The efficacy rate in terms of the number of improved cases for seborrhea was 92.9% (13 cases) at T0, and 71.4% (10 cases) at T7. On the other hand, the efficacy rates of simple bubble bathing without ozone for seborrhea were 33.3% at T0 and T7. The overall score improvement rate of seborrhea was 70.0% (p<0.01) at T0, and 40.0% (p<0.01) at T7. The effectiveness of the treatment was also assessed by the owners using a Visual Analog Scale. The efficacy rates were 85.7% at T0 and 78.6% at T7, and the overall improvement rates were 60.6% (p<0.01) at T0 and 37.9% (p<0.05) at T7. No adverse events or dropouts were observed during the study period. The results indicate that ozone-bubble bathing is clinically effective in treating seborrhea in dogs.

Sebaceous adenitis (SA) is an immune-mediated dermatosis predominantly affecting canine breeds like the Standard Poodle and Akita. The clinical manifestation of SA and its treatment outcomes differ among breeds. A retrospective study on the incidence of skin diseases in the Havanese dog breed in the United States (US) indicates that SA might be overrepresented in this breed. Though the population of the Havanese breed has been growing in the US, the population size of this breed is relatively small in Japan and its susceptibility to SA is not well known. We have encountered several cases of SA in Havanese dogs, all of which shared the same male parent. Here, we report the clinical presentation of these cases as well as their treatment outcomes and family tree of the affected dogs.

An 8-year-old intact male Chinese Crested Dog presented with multiple nail abnormalities, including onycholysis, onychomadesis, nail dysplasia, hemorrhage, lameness, and pain. Skin symptoms, such as pruritus, erythema, and papules, were also noted on the neck and back regions. Symmetrical lupoid onychodystrophy (SLO), potentially associated with an adverse food reaction (AFR), was suspected based on clinical presentations and the exclusion of other differential diagnoses through histopathology and diagnostic testing. A novel protein diet trial resulted in significant improvement in both the pruritus and nail lesions. The recurrence of pruritus following a dietary provocation test supported the involvement of AFR in this case. This case highlights the potential association between SLO and AFR, emphasizing the importance of dietary management for dogs with pruritic skin disease and nail abnormalities.

A 14-year-old castrated male Shiba dog presented with a 6 cm-diameter mass in the left cervical lymph node. Histopathologically, the mass consisted of sheets of neoplastic mast cells and diffuse eosinophils. The neoplastic cells exhibited marked atypia including anisocytosis, a high mitotic index, karyomegaly and multinucleation. Some neoplastic cells had phagocytosis of 1–7 eosinophils in the cytoplasm. Immunohistochemistry revealed that the neoplastic cells were positive for anti c-kit antibody, and based on the above findings, a mast cell tumor was diagnosed. Eosinophils were observed in the cytoplasm of the tumor cells by double staining with Luna stain and immunostaining for anti c-kit antibody.

Topical therapy for canine superficial pyoderma is recommended to limit the use of systemic antimicrobial agents due to the increasing prevalence of multidrug-resistant staphylococci in recent years. We evaluated the efficacy of a commercially available topical formulation containing totarol in 10 cases of canine superficial pyoderma. A significant difference between median clinical scores before and after the 14-day trial was observed (p=0.00742). In 77.8% of cases, a 75% or more improvement in clinical scores was achieved. These results indicate that treatment with this formulation may simplify the management of localized superficial pyoderma.