The effect of ovariohysterectomy under the halothane anesthesia on canine neutrophil function was investigated. The ovariohysterectomy was performed in 13 clinically healthy female dogs. Nitroblue tetrazolium (NBT) reduction test was performed before and after operation. The percent positive cells in NBT reduction test without stimulation (resting NBT reduction test) was significantly decreased immediately after operation (p<0.01) and at 1 day postoperation (p<0.01) . The NBT reduction test with stimulation with endotoxin (stimulated NBT reduction rest) was significantly decreased immediately after operation (p<0.001) and at 1 day postoperation (p<0.001) . The difference in percent positive cells between stimulated and resting NBT test at each sampling time was also significantly decreased immediately after operation and at 1 day postoperation (p<0.01, respectively) . The decrease in the percent positive cells of NET reduction tests and the difference between stimulated and resting NBT test recovered to the preoperative values at 7 days postoperation. The results suggest that NBT reduction ability in canine neutrophils is suppressed after ovariohysterectomy under the halothane anesthesia.

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Minute electric current caused in the inflammatory changes at the trauma between the two electrodes, as well as at the site of both electrodes. The inflammation was intensified irrespective of the polarity of the electrodes, and in three days the signs of acute inflammation was apparent, including marked edema, hyperemia and infiltration with neutrophilic granulocytes, histiocytes and monocytes. The blood vessels around the lesion were found to be distended. After the end of electrical stimulation, the extent of inflammation was reduced to the level before the electric stimulation. This inflammation, mainly characterized by hyperemia and edema, was considerd as follows. When electricity was applied, the current which flowed in the blood stream and extracellular fluid intensified the inflammatory changes by transferred ions. Thus, the local temperature rose by granulocytic pyrogens and hyperemia, and histamine like substance and serotonin were released. Then the vascular permeability was increased and induced the maintenance of the increase in acute inflammatory cellular infiltration.

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A one-year-old female mixed breed cat was presented with a prolonged history of the right forelimb lameness. Physical examination revealed pain at the right forelimb by palpation and an open wound was observed at the right armpit, from which the discharge of bloody pus was observed. Radiography of the right forelimb revealed bone lysis and regeneration at the medial proximal humerus, which supported a diagnosis of chronic osteomyelitis. The necrotic, lytic bone and surrounding soft tissue were removed under the general anesthesia. A drainage was attached to the bone defect. After operation, the satisfactory result was obtained by administration of tetracycline based on the antibiotics sensitivity test for about one week.

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