For higher utilities of the proteins obtained from the dried and activated sludge of high ash contents, the conditions economically and technologically favorable for the partial hydrolysis by acid were investigated. The hydrolysates of the dried sludge obtained under the conditions differing in the concentrations and the volumes of the acids, and in the time intervals for the hydrolysis were subjected to the determination of the soluble- and ammoniacal-nitrogen, trinitrobenzen sulfonate positive substances and 18 kinds of amino acid.
Among the conditions thus investigated, the followings were concluded to present the adequate results for the amino acid recoveries: 1.05kg/cmcm² of the autoclaving pressure, 4 hours for heating, 4-6N of hydrochloric acid (over 270mg per g of dried sludge) or sulfuric acid (over 290mg per g of dried sludge).

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Soybean proteins consist of four ultracentrifugal components, which are capable to be fractionated by means of dialysis. The viscotic and electrophoretical behavior of each component was studied to obtained the information about their interaction and properties. The viscosity of native protein and urea denatured protein increased hyperbolalike curvature with protein concentration, and of urea denatured protein showed great concentration dependence. After the viscosity of sample solution reached about maximum, the viscosity decreased rapidly by the addition of 2-mercaptoethanol. Same result was also obtained by the use of guanidine·HCl and cystein as denatured and reduced reagents, instead of urea and 2-mercaptoethanol. The viscosity in urea increased linearly with urea concentration, and increased with time. The dissociated subunits of C component were seen in more than 2M urea concentration on starch gel electrophoretical patterm without urea. The result corresponded to the fact that the decrease of viscosity due to the dissociation was seen in more than 2M urea. The dissociation of D component was seen in more than 4M urea on the electrophoretical pattern. The C component was much more denatured by urea and guanidine·HCl than the D component.

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The Phospholipids compositions of three parts (unpolished, polished rice and bran) of nonglutinous rice were investigated comparatively. First, lipids were extracted from each materials with the solvent mixture of chloroform-methanol (2:1) at low temperature. Then, these were treated with cold acetone and conjugated lipids were obtained in acetone-insoluble fraction.
Identified or presumed phospholipids were phosphatidyl choline, hosphatidyl ethanolamine, phosphatidyl serine, lysophosphatidyl choline, lysophosphatidyl ethanolamine, lysophosphatidyl serine, phosphatidyl inositol, phosphatidyl glycerol and phosphatidic acid, but phosphatidyl serine and phosphatidic acid were not found in polished rice.
Total phospholipids were 1.41% in unpolished, 0.65% in polished rice and 5.16% in bran, and the ratio of distribution to polished rice and bran was 39.7:60.3. Only lysophosphatidyl choline of polished rice was more than that of bran.
Fatty acids compositions of these were mainly C₁₈-unsaturated acids.

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The biological evaluation on the feeding value of autoclaved rapeseed meals (Brassica napus defatted seedmeals and B. compestris seed oilmeals) was studied employing chick and immature rat. In addition the influence of the autoclaving on the activity of the trypsin inhibitor in rapeseed meals was investigated by in vitro experiment.
No detrimental effects of the autoclaved rapeseed meals were observed when the amount of rapeseed meals was 20% level, although the untreated meals showed unfavorable effects by marked thyroid hypertrophy and depression of body weight gain. Much of the trypsin inhibitor in rapeseed meals was also destroyed by the autoclaving. In comparison of the toxicity of the untreated meals, the detrimental effiects of B. campestris seed oilmeals were generally lower than those of B. napus defatted seedmeals.
These findings suggest that the autoclaving destroyed both the goitrogenic substances and the groth inhibiting factor (s) in rapeseed oilmeals resulting in the remarkable improvement of feeding value of the meals.

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A simple analytical method of agar in seaweeds was investigated, and the contents of agar in some Gelidium sp. harvested in Japan and foreign countries were estimated by this method.

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In order to prevent the loss of lysine during soaking, gelatinization by steamilg and retrogradation by cooling of the enriched rice did not give satisfactory results. As the resuit of coating test employing an automatic film coating machine, it was revealed that zein-palmitic acid mixture (4:6) formed good coating film on the rice and the amount of lysine eluted into water was less than 5% after ten min. soaking when 3% of the coating agent was used.
Stearic acid and other higher saturated fatty acids showed the same effect when used instead of palmitic acid.

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