Albumins and globulins in the endosperm of uruchi-mai (regular rice, non-glutinous rice) and mochi-mai (waxy rice, glutinous rice) were examined by gel-filtration and starch-gel electrophoresis. Amino acid composition and ultraviolet absorption were also determined.
Albumins of uruchi-mai were separated into four fractions with molecular weight of 12, 000, 18, 500, 26, 000 and 76, 000, respectively. Albumins of mochi-mai were separated into five fractions with molecular weight of 10, 000, 18, 000, 25, 000, 54, 000, and 117, 000, respectively. Both of the globulins of uruchi-mai and mochi-mai were separated into four fractions with similar molecular weights. The molecular weights were estimated to be 16, 000, 66, 000, and 127, 000 for the three of their fractions, respectively. The rest, the smallest one, was not estimated. The amino acid composition of the albumins and globulins manifested similar values, respectively, between uruchi-mai and mochi-mai with one exception of lysine content of their albumins. Small amounts of the contaminants of the nucleic acid-related compounds in rice endosperm globulins were detected by ultraviolet absorption spectrum. The albumins gave 14-17 bands on acidic or alkaline gels on starch-gel electrophoresis, while the globulins gave 9 bands. Uruchi-mai and mochi-mai gave a similarity in the starch-gel electrophoresis, but a few clear differences were detected in the number, position, and color accent of the bands.
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