The molecular species of 3-sn-phosphatidylethanolamine from soybean were determined after convertion into diglyceride acetates by means of hydrolysis with phospholipase C and acetylation with acetic anhydride and pyridine. Employing columnchromatography on silica impregnated with silver nitrate, the resultant diglyceride acetates were separated into 15 (EDGA-1-9) differing with respect to their degree of unsaturation. Furthermore, the diglyceride acetates were separated by gas liquid chromatography on 3%-JXR on the basis of total carbon number of the fatty acids.
As the results, the amount of the 3-sn-phosphatidylethanolamine composed of total carbon number 28 (14+14, 12+16), 36 (18+18, 16+20) and 38 (18+20) was abundant, and it was assumed that the original 3-sn-phosphatidylethanolamine contained linoleic acid (49.8%), palmitic acid (28.2%) and stearic acid (1.5%) in large quantities. Notwithstading the original 3-sn-phosphatidylethanolamine contained arachidonic, eicosatrienoic and eicosadienoic acid in comparatively large quantities, the amount of the 3-sn-phosphatidylethanolamine composed of total carbon number 40 was scarcely, and it was assumed that the fatty acids of the 3-sn-phosphatidylethanolamine among both ester position were not only unsaturated ones composed of carbon number 20, but also ones composed of except carbon number 20, for example (18+20), (16+20) and (14+20).
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