The changes of color and total anthocyanin in the concentrated juice of Campbell Early grapes which was stored at room temperature, 5°C and -30°C, were investigated for ten months.
Optical density of the juice(at 520nm)and the total anthocyanin content decreased rapidly as follows: room temperature>5°C>-30°C. At -30°Cfor ten months, the color density and the total anthocyanin content decreased to78% and 58%, respectively. In Six bands of anthocyanins on paper chromatogram, band 4(3-monoglucosides of malvidin and peonidin)decreased to 4%, band 3 (peonidin-3, 5-diglucoside and petunidin-3-monoglucoside)to 8% and band 2(malvidin-3, 5-diglucoside and delphindin-3-monoglucoside)to 9%, at room temperature for ten months. From these evidence 3-monoglucosidic anthocyanidins seemed to decompose more easily than 3, 5-diglucosides in the grape juice.
Crude anthocyanins prepared by basic lead acetate method were isolated by paper chromatography and characterized by spectroscopy, hydrolysis and chromatographic techniques. Nine anthocyanins were identified: 3-monoglucosides of malvidin, peonidin, peptunidin and delphinidin, 3, 5-diglucosides of malvidin, peonidin and delphinidin, and malvidin-3-monoglucoside and peonidin-3, 5-diglucoside acylated with
p-coumaric acid.
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