Glyphosate was applied to nine lines of
Equisetum arvense L. (Table 1), and there were considerable differences in the GR
50 value among the lines from different localities. The GR
50 value of the most tolerant line was 4.93kg a. i./ha, 2.3 times higher than that of the most sensitive line (Table 2). These results suggested that the variability in the control of
E. arvense L. observed in the field might be due to the occurrence of lines which differ in their susceptibility to glyphosate.
A new rapid method for the assay of the susceptibility to glyphosate was developed (rhizome-incubation-method). The procedure is as follows. At first, the rhizomes of
E. arvense L. are dipped into a glyphosate solution for about 30 seconds and put on a petri dish (15-cm-diameter. with the bottom of each petri dish covered with a filter paper). Then 30ml of glyphosate solution is poured on the petri dish and the number of sprouting shoots is counted after a three-week incubation at 20°C, under dark conditions. The results of the application of this method were similar to those of pot trial (Table 3).
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