One hundred grams of unhulled rice or 20 grams of rice hull were extracted with 1000ml water at 5°C during 48 hours. Thereafter, the water was filtered and diluted twofold with distilled water and was used for 0.5% agar culture media in plant boxes, on which seeds of
Monochoria vaginalis were sown at 1mm depth. Distilled water was used for the 0.5% agar culture media in the boxes as untreated control.
M. vaginalis seeds germinated well at incubation temperatures ranging between 20 and 25°C in the agar media of the water extract of unhulled rice. The length of the seminal root was maximum at a 30°C incubation temperature in the agar media (Table 1). Seed germination rates of
M. vaginalis were high in the agar media of the water extract of unhulled rice diluted two or four times (by volume), and were also high in the agar media of the water extract of rice hull diluted two times (by volume) (Table 2).
Seed germination rates and seminal root and cotyledon length of
M. vaginalis were determined after the species were cultured in agar media consisting of the water extract of unhulled rice of 43 rice cultivars including Japonica and Indica types at 30 (light) -25 (dark) °C during seven days. The germination rate of
M. vaginalis seeds in the agar media of the untreated control was 13.3%, while these in the agar media of the water extract of unhulled rice of all the cultivars ranged from 78.3 to 96.7%. Seminal roots of
M. vaginalis seedlings elongated more in the agar media of the water extract of unhulled rice of all the cultivars except for three cultivars, compared with the media of the untreated control. Cotyledons of
M. vaginalis seedlings grew longer in the agar media of the water extract of unhulled rice of all the rice cultivars than in the media of the untreated control (Table 3).
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