There are many experiments in which changes specifically in lymphatic tissues could be produced by lymphotoxins and so forth. Prof. Maekawa predicted from his own new theory on allergy that the phosphatide of lymphocyte or lymphnode would act as a determinant group in the specific allergic changes of lymphatic tissues. In order to ascertain the role of lymphnode phosphotide, I performed several experiments, using various combinations of phosphatides and ox sera for sensitization and reinjection. The results of these experiments seem to show that the lymphnode phosphotide acts as a "hapten". The phosphatide is considered as a determinant group of the antigen which restrict allergic chaeges to the lymphatic tissues, while the ox serum is as a transporter. When rabbits were sensitized with ox serum alone or with the mixture of ox serum and lymphnode phosphatide and reinjected with the same mixture, specific allergic changes occurced in the lymphatic tissues ("Lymphon-Allergy") but if reinjected with the ox serum alone, the "Lymphon-Allergy" did not occurred.

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Venous wave and auricular wave investigated in normal respiration, in deep inspiration, in deep expiration, in Muller and Valsalva-test. (1) Depthes of X-valleys in venous waves were always larger in inspiration than in expiration. (2) In auricular wave, depth of Sm-wave was only slightly larger in stoppage of respiration immediately after deep inspiration than in that condition after deep expiration [This does not stand in contradiction with (1): those conditions were thought to be the mixture of deep inspiration and Valsalva-test, or that of deep expiration and Muller-test.]. (3) In Muller-test, depth of Sm-wave was remarkably increased and remarkably decreased in Valsalva-test. These experiments show that venous return is accelerated by the conditions which decrease intrapleural pressure and suppressed by reverse conditions, i.e. venous return, caused by suction-mechanism of heart, is helped by respiration.

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The experiment was performed by intratesticular innoculation with suspension of hemolytic streptococci 31 times for relatively long period and intravenous reinjection of small volume of cocci combined with rabbit-heart-muscle phosphatide. Electrocardiogram showed T-descendence since the 10th injection in some cases and T-descendence or disappearance since the 30th in most cases. C.P.R. (Cor Phosphatide reaction) of sera became positive. Allergic interstitial myocarditis with periarteritis nodosa was obtained by focal infection in testicles.

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Maekawa expresses the opinion that in the allergic diseases phosphatide from any organ or tissue makes the antigen combine with that organ or tissue. Therefore, I expected that when rabbits were sensitized by the mixture of streptococci viridans and rabbit-heart-muscle or men-heart-endcardium-phosphatide, the cocci would adhere to the heart to produce an allergic carditis. On the first experiment I sensitized rabbits by the intertesticular injection of the streptococci viridans with rabbit-hert-muscle-phosphatide. Clinically body weight, body temperature, blood pressure, blood picture and urines were not so changed, but electrocardiogram showed myocardial damages CPR-reaction by Araki showed positive and complement in the blood decreased. After killing the rabbits, I investigated histologically all organs of them, especially heart. I found more remarkable allergic interstitial myocarditis at the experimental rabbits I found a large basophilic monnuclear cell in the perivascular plasma cells. The purulence and incease of the connective tissure were recognized in the testicles of all animals.

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