We electrophorized the serum (plasma) ptoteins of hypertensive patients, and experimental renal, endocrine, electrolytic and pressorreceptor denerved hypertensive dogs and rats, and observed a correlation between hypertension and serum globulin, especially alpha-2 and betha globulins, and obtained the following results.(A) Human 1) Essential Hypertention In the stabile and benign form, there is generally little change of serum protein, and in younger ages alpha and gamma globulins increase slightly, and betha globulin and A/G ratio are normal. In fourties A/G ratio decreases slightly, and in more fifty years old, alpha and gamma globulins are normal and betha globulin increases and A/G ratio decreases. These findings suggest that the increase of betha globulin relates to ages, that is to say, arterio-sclerosis. In the malignant stadium, alpha and betha globulins increase and changes of other components of serum protein are not remarkable.2) Renal Hypertention Alpha globulin increases and A/G ration decreases.3) Hypertention accompanied with Heart Insufficiency. In many cases alpha globulin increases, and in all cases betha globulin increases, and also gamma globulin increases and A/G ration decreases.4) Hypertention accompanied with Cerebral Bleeding. Alpha and betha globulins increase slightly and gamma globulin is normal and A/G ratio decreases slightly.5) Control Group In normal tensive cases over 60 years old, alpha, betha and gamma globulins increase slightly and A/G ratio decreases.(B) Dog In GOld-blatt's and Page's dogs, dogs with ligated carotid arteries, and adrenal veins, dogs treated with kidney ATP-ase plus cholesterol etc., we examined a correlation between blood pressure and fractions of serum proteins, and obtained the following results.Generally, in the dogs, which hypertention occuren, betha globulin inclines to increase. In the dogs treated with kidney ATP-ase and cholesterol, gamma globulin increases gradually.(C) Albino-rat In salt hypertensive rats, betha globulin inclines to increase. In DCA hypertensive rats, no remarkable changes were observed.We can summarize these results as follows.Generally, in hypertension there are only slight changes of the fractions of serum (plasma) protein. In dogs it seems that there is a slight correlation between hypertension and betha globulin, but in human there is no correlation between the two, and we think rather that there is a correlation between betha globulin and ages, that is to say, arteriosclerosis. We think that other changes of serum protein have no direct relation to hypertension, and are based on secondary occured diseases.

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In the present investigations I designed to determine the relationship between experimental hypertension and NaCl-metabolism, cousidering a role of Na-factor in pathogenesis of hypertension.I devided my report in three parts as follows; I Introduction, DCA hypertension, and acute Salt-hypertension, II Chronic Salt-hypertension, III Disucussion and Summary.Then, using male albino rats, I succeeded in producing DCA hypertension and acute salt-hypertension, and investigated various clinical observations such as 17-KS in urine, blood pressure, & NaCl balance. The animals (DCA-Group) were injected with DCA 5 mg. daily or every other day. Blood pressure of rats was measured by tail plethysmograph. Thereafter, the animals were killed, and then organs were histologically investigated. Adrenals were also histochemically observed.The toxic actions of DCA were remarkably influenced by the presence of the sodium chloride content of the diet, as the previous reports (Selye).In relation to sod. chloride metablism, elevation of blood pressure due to overdosage of DCA was observed after the retention of NaCl, and preceded the nephrosclerosis in rats.The present experiment suggest that both DCA- and Salt-hypertension in rats are very similar in relation to developing through the retention of NaCl.

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ARAKI has reported a possibility to diagnose the my cardial damage serologically by means of the myocardium phosphatide flocculation test (CCR), and HANGER has reported that the cephalin-cholesterol flocculation test (CCF) is a sensitive indicator of parenchymal liver cell damage. The auther divided bovine myocardum phospatide into cephalin which is insoluble in cold pure alcohol but soluble in ether and lecithin which is soluble in cold pure alcohol, and studied on the myocardium cephalin-cholesterol floculation reaction (CCR) to know the possibility to diagnose myocardial damge. The results of CCR were compared with the findings of ECG. Further I carried out the quantitative analysis of myocardium cephalin in patient's serum serologically by the myocardium cephalin precipitation reaction (ACCR) using anti myocardium cephalin rabbit sera.127 patients were subjected to these tests. 50 of 55 patients with myocardial damage revealed by ECG showed positive result by CCR and 5 patients negative. 41 of 72 patients without myocardual damage showed negative and 31 patients positive. Namely in 91 of 127 patients (71.6%) the result of CCR was identical with the finding of ECG, but for the purpose of the diagnosis of myocardial damage CCR was a sensitiver indicator than ECG. When CCR is carried out serially during the course of a disease, it will be a useful method to know the prognosis of myocardial damage. The result of CCR by human myocardium cephalin concided with that by bovine myocardium cephalin. This indicates that the myocardium cephalin has no species-specificity.A clear correlation between CCR and the myocardium lecithin-cholesterol flocculation reaction (CLR) was found, but CCR was sensitiver than CLR. There was a parallel relation between CCR and CPR, but 63% of the result of CPR coincided with the finding of ECG, therefore, CCR was a sensitiver indicator of myocardial damage than CPR. The results of CCR were correlated slightly with those of CCF.In patients who showed the positive reaction of CCR the titer 1 : 3200 of ACCR was mostly found, but in those who showed the negative the titer 1 : 2000 of ACCR was mostly found. The boundary precipitation titer between the positive and the negative reaction of CCR was 1 : 2400, but that between the patients with myocardial damage revealed by ECG and the normals was 1 : 3200. The quantity of myocardium cephalin in sera of patients with myocardial damage increased, namely, the high precipitation titer of ACCR was obtained. The antigen titer in the myocardium lecithin precipitation reaction (ACLR) was lower than that of ACCR, but there was a clear correlation between the both tests. These facts may be probably due to the incompletion of the division technique of cephalin from phosphatide.

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