1. When electrolytic lesions were produced in bilateral anterior-hypothalamus in the dog, TRF almost disappeared from the body and could not be detected in the neurohypophysis, cerebrospinal fluid and blood as well as in the anterior hypothalamus. Nor was it excreted in urine.
2. These results are in accordance with the previous report that TRF was present only in the extract of the anterior hypothalamus, but not in the other parts of the central nervous system. It was therefore concluded that the site of Production of TRF is the anterior hypothalamus.

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Prolactin was injected to the normally lactating and unilaterally ligated mice in order to ameliorate or maintain the respiratory activity of the mammary gland From the results of the histological observation and litter's growth curve in addition to the respiratory activities, it was suggested that prolactin might exert some influence on metabolic or synthetic process, qualitatively but not quantitatively. And it was discussed that there might exist a certain optimum dose according to the condition of mammary gland for prolactin to exert stimulatory effect on the mammary respiratory activity. However, prolactin, in adopted doses, did not prevent the involution occurring in the ligated gland six days after unilateral ligature. From these results it may be possible to conclude that the removal of milk might be a limiting factor, and further, prolactin may be effective for maintaining the mammary gland in the functioning state, although the dosage of hormone in connection with the stage of lactation is an important factor.

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In ovariectomized rats, daily injections of 2mg of 17α-ethinyl-19-nortestosterone induced vaginal estrus (vaginal index, 5) continuing for several days. But after this, vaginal indices fell rapidly and animals came into anestrous state. Subsequently, however, indices rose gradually again up to 5 (full estrus). Smaller doses of the steroids (0.125 and 0.5mg daily) invariably led to continuous estrus, interruption failing to occur. The sequence of events is quite comparable to that occurring in ovariectomized rats receiving daily injections of 2mg progesterone and 0.125mg estrone concurrently (Maekawa, 1955). It seems highly probable, therefore, that progestational property of 17α-ethinyl-19-nortestosterone interferes with estrogenic action of the same steroid as progesterone prevents the effects of estrogen administered simultaneously.

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A procedure of isolating a biologically active protein from the human mixed saliva was described. The acetone precipitate (S-precipitate) of the human saliva extracted with glacial acetic acid yielded E-precipitate by addition of ether.
By purifying the E-precipitate a new strong biological active substance was isolated and named “Saliva-parotin-A”. The yield was 25 to 30mg per 100ml of mixed saliva.

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The physicochemical properties of Saliva-parotin-A were investigated. It gave a positive reaction to all coloring and precipitating reactions of protein.
It had an isoelectric point of pH 5.4-5.6, but did not precipitate at that pH.
Purity by electrophoresis was shown to be 90%(by ascending pattern), but by ultracentrifugal analysis a single boundary was obtained.
Ultraviolet absorption maximum was at 277.5±0.5mμ and the polarogram in the presence of Co⁺⁺or Co⁺⁺⁺did not produce a protein wave.
The total nitrogen was 15.21%. It did not contain sulphur and ash. It consisted of 15 kinds of amino acids with the exception of sulphur containing amino acids.
As to the biological properties of Saliva-parotin-A, the minimum effective dose for rabbit serum calcium decreasing activity was 50 to 100μg; for circulating leucocytes decreasing and increasing activity 5 to 10μg; for calcification of rabbit incisor dentine 10μg.
Saliva-parotin-A is a different kind of protein from Parotin so far as the physicochemical properties are concerned.

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Tadpoles of Bufo vulgaris formosus were kept in a 0.05% thiourea solution for 87 days, and the effects on the eye-balls, thyroid glands and gonads were histologically examind.
Exophthalmos produced by a striking enlargement of the eye-ball was observed in different degrees in the majority of the treated tadpoles. The thyroid glands of those tadpoles remarkably increased in size, and grew into Struma parenchymatosa.
Gonadal differentiation was more advanced as compared with the frogs of the same age collected in the field, although degenerative changes were observed in the ovaries of some tadpoles. Development of Bidder's organ was considerably inhibited. Sex ratio was 1:1 as a whole, although severe exophthalmos was mostly observed in females.
Masculinization of the genetic female following treatments with goitrogens seems to occur easily only in higher anurans such as Rana and Rhacophcrus, and such a reversal seems to be difficult to occur in urodeles and lower anurans.

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By means of isoelectric precipitation, ethanol fractionation, and alumina treatment method, uroparotin was isolated in a homogeneous form from human urine. The yield of uroparotin was shown to be 1-1.2mg per liter for α-uroparotin and 0.8mg per liter β-uroparotin. Uroparotin was considered to be a typical glycoprotein, containing sialic acid as one component of carbohydrate moieties. Neither a proteolytic effect nor a blood pressure depressing effect was observed in uroparotin, nor other hormonal actions. Therefore, uroparotin may be considered a new glycoprotein having characteristic biological effects. No difference in properties of both α-and β-uroparotin could be found, and the mechanism of the biological effects remains to be clarified.

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Carbohydrate components of uroparotin were analyzed by paper chromatography and quantitatively determined by the colorimetric method. It became that carbohydrates of uroparotin consisted of mannose, galactose, galactosamine and sialic acid, but fucose and uronic acid were not detected. The amount of carbohydrate moieties was estimated to be about 24%, and hardly difference could be found between α-and β-uroparotin.

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The distribution of the alanine-α-ketoglutaric transaminase of the rat and of the tyrosine-α-ketoglutaric transaminase of the pig was observed among the thyroid, adrenal cortex and medulla, adenohypophysis, liver, kidney and heart. The content of pyridoxal of these tissues was also measured.
These two transaminases were highly active in liver and kidney of both rat and pig. Pyridoxal was also abundant in these two tissues.
Transaminases and pyridoxal were both small in amount in the heart and in the endocrine tissues in general.
Among the endocrine tissues examined, the pig thyroid did not show any activity of tyrosine-α-ketoglutaric transaminase. This fact was found to be caused neither by the lack of coenzyme nor by the presence of any enzyme inhibitor, but actually due to lack of the apoenzyme.
Low activity of the thyroid and high activity of the liver and kidney of the transaminases were considered significant in connection with the production and the breaking down of thyroid hormones in these organs.

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