1) The effects of castration for 25days and norandrostenolone phenylpropionate (NAPP) administration to these castrated animals on the glycogen contents of the liver and rectus femoral muscle were studied. Following the castration remarkable decrease of tissue glycogen was found, which was somewhat prevented by the treatment with NAPP. The ratio of TCA extractable glycogen to total one was almost invariable in every case.
2) One mg daily of TP, NAPP, cortisone and cortisone plus TP respectively was injected to young male rats for 3days.
The administration of TP and NAPP showed no influence upon the liver glycogen content but in the muscle a considerable increase of glycogen was observed, which was mostly due to an increase of TCA extractable one.The administration of TP in addition to cortisone had little influence upon tissue glycogen already increased markedly by cortisone.
3) After the injection of glycine-¹⁴C higher ¹⁴C-specific activity of tissue glycogen was noted in the animals treated previously with NAPP than in the castrated. The incorporation of ¹⁴C due to ¹⁴C-glycine administration was observed in liver glycogen first and 3-6 hrs. later in muscle glycogen.
Higher radioactivity was found in the residual glycogen than in the TCA extractable one in every case.
Therefore it might be suggested that anabolic steroids such as NAPP may have the action to accelerate gluconeogenesis from glycine and other amino acids.
Some mechanisms of androgens on carbohydrate metabolism, especially on the gluconeogenesis from amino acids were discussed.

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Goldthioglucose was intraperitoneally injected into Bufo vulgaris japonicus which was collected in July, August and November.
The evidences obtained from this experiment were as follows:
1) After the injection of goldthioglucose, a large amount of CH-positive substance appeared in the neurosecretory cells, axones and interstitial tissues in the preoptic area. In axones, so-called “perlschnurartige Anordnung” was exhibited.
a) The quantitative change of neurosecretory substance in the preoptic area was diphasic or more.
b) The susceptibility of the preoptic nucleus to goldthioglucose varied with sex of animal and the season in which the experiment was performed.
2) The action of goldthioglucose can be considered to be due to its gold molecule.
a) The injection of gold chloride increased. CH-positive substance in the axones.
b) The injection of sodium thioglycollate did not elicit any remarkable change in the preoptic area.
3) Two kinds of neurosecretory substance which were distinguishable with azan staining were in both positive to PAS reaction even after digestion with saliva.

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Large doses of progesterone, testosterone, androsterone, Δ⁴-androstenedione and dehydroepiandrosterone were administered in pregnant rats to elucidate the action of these sex steroids disturbing pregnancy. Absorption or death of the fetus was observed by the administration of a relatively larger doses of these steroids, while various degrees of masculinization of the genetic female offspring was produced by the administration of smaller doses of these steroids except dehydroepiandrosterone. The degree of masculinization was found to be related with the dose of these sex hormones and also with the period of pregnancy during which they were administered. The highly modified fetus and offspring have oviduct, upper vagina combined with epididymis, vasa deferentia, seminal vesicle, prostate and penis.

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Carbohydrate components of α-parotin were analyzed by paper chromatography and determined by the colorimetric method. It was found that carbohydrates of α-parotin consisted of galactose and galactosamine. The total amount of carbohydrate was estimated to be about 16-18%. N-terminal amino acid residues were analyzed for α-parotin. It was found that the N-terminal amino acids of α-parotin were aspartic acid, alanine and lysine.

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α-Parotin was distinctly more effective on its hypocalcemic, leukokinetic, and calcification activities than parotin. The optimum dose of α-parotin on the calcification of rabbit incisor dentine was considered to be 50-100μg per kg.α-Parotin was unstable against heat and acid.α-Parotin also was inactivated by ketene, nitrous acid, formaldehyde, and iodine, but not by cysteine and thioglycollie acid. Amino and tyrosine-phenyl groups in α-parotin molecule were responsible for its biological activities.

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Marked inhibition of weight-increase in the salivary glands and atrophy of the thymus were simultaneously observed in both male and female rats by the administration of the thioureylene-type antithyroid drugs.But considering the fact that the atrophy of the thymus experimentally induced by the administration of corticosteroid or testosterone did not result in a weight-decrease in the salivary glands and that, inversely, atrophy of the thymus was not always observed during a given period of salivary-ectomy or duct-ligation of the submaxillary glands, it seems, that there is no direct relationship between the thymus and the salivary glands. Hence, the marked change in the weight of both organs observed in the administration of an antithyroid drug is considered to be independent of each other.

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The serum antidiuretic activity in female rats was determined after ovariectomy and pregnant mare serum gonadotropin administration with following results.
1) The serum antidiuretic activity of spayed rats was higher than the control.
2) The serum antidiuretic activity of rats injected with pregnant mare serum gonadotropin was not significantly different from the control.
3) The interrelationship was discussed among the amount of neurosecrete, the pituicyte activity and the serum antidiuretic activity after these treatment and a possible role of the pituicyte for the release of the antidiuretic substances was stressed.

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Cultivation of the rat anterior pituitary cells is successful by the present methods for longer term and in more successive generation than ever reported.
Evidence has been presented that the hypothalamic tissue containes certain substance favouring the increase in gonadotropic activity of the anterior pituitary cells cultivated or their medium while the brain cortical tissue has no such substance.

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