When chlorpromazine is administered to women in both the early and the later stages of normal pregnancy, with toxemia of late pregnancy or in postclimacteric age, the excretion into urine of estrone, estradiol, estriol, pregnanediol and total 17-hydroxycorticoid was found reduced, but that of total 17-ketosteroid was little affected.
Following administration of chlorpromazine, the values not only of excreted pituitary but also of chorionic gonadotropin were decreased, the action of ACTH in stimulating the adrenal cortex was lowered and the response to Friedman's test was inhibited. Consequently, it is inferred that chlorpromazine, beside depressing the center of internal secretion, simultaneously reduces the function of the peripheral endocrine organs as well as of the placenta.

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A fluorometric method in combination with the silica gel column chromatography by Sweat for the estimation of cortical hormones was reinvestigated under a slight modification by the author. And with specimens of human being and some animals, normal values were given by this method.
The fluorogenic substances contained in the fractions of human, rat and guinea-pig plasma, were analysed further by the paper chromatography, and the following results were obtained;
a) In human being the so-called Cpd F-like substance appears to indicate practically the level of hydrocortisone itself, but in the so-called Cpd B-like substance, somewhat other substances besides a small amount of corticosterone are contained.
b) In rat plasma corticosterone made the chief component of the so-called Cpd B-like substance in which the presence of a small amount of Reichstein's Substance S was also detected.
c) In guinea-pig plasma hydrocortisone, which is detectable in the Cpd Ffraction, appears to be the major cortical hormone; corticosterone and the other known active principles were not found in the Cpd B-fraction.

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Assay was made of the gonadotrophic potency of the anterior pituitaries obtained from cocks, laying hens and non-laying hens in annual molting, of the Single Comb White Leghorns and partly those of the crossbreds. The degree of response was measured by the increment of the testicular weight of one-day-old cockerels.
Pituitaries from cocks were the richest in gonadotrophin content and pituitaries from non-laying hens showed a greater potency than those obtained from laying hens.

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Bates and Cohen (1950 a, b) and jayle and Crepy (1952) have already reported on the microfluorometry of estrogens a few years ago. Fluorescence spectra were measured comparatively precisely and reported by Slaumwhite et al. (1953) and by Aitken and Preedy (1953). As for the limit of such measurements, Braunsberg et al. (1954) reported that a very minute amount could be detected.
The present authors also reported on the fluorescence spectra of natural estrogens (Nakao and Aizawa, 1955) and described the method whereby the minimum amount could be determined.
Numerous reports have appeared regarding the method of isolation of natural estrogens, such as the chromatography (Stimmel, 1944) and counter-current distribution (Engel et al., 1950) and more especially the microchromatography by Braunsberg et al. (1954).
The present paper describes the separatory determination of estrogens in the blood and urine established by the writers.

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1. D, L-thyroxine (final concentration: 1×10^-4M) activates succinoxidase activity of mouse liver homogenate with addition of exogeneous cytochrome c, only in medium of narrow pH range around 7.4. Such effect of the hormone is not remarkable in pH 7.0 and 7.9.
2. No effect is found on the enzyme system with addition of L-diiodotyrosine and L-tyrosine in the same molar concentration with thyroxine (1×10^-4M) at all pH employed.
3. From the data of spectrophotometric observation in ultraviolet region, solubility of thyroxine decreases with sharp gradient from pH 9 to 7.

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1. The effects of Parotin on the development of 7-and 9-day chick embryo femora were studied in tissue culture.
2. Pair-mate explants were cultivated by roller-tube methods with use of slightly modified “dynamic medium” and demonstrated a histological and anatomical development. One of each pair was cultured in the plus-Parotin medium with various concentrations.
3. These experiments resulted in the following;(a) in the concentration of 15mg per cent region in the medium, it was found Parotin to have stimulative action on the longitudinal growth of the explants, (b) in the same concentration promotive effect on the deposition of calcium was observed when 9-day chick embryo femora was employed, (c) in a higher concentration, inhibitory effect was found remarkably in both processes of ossification.
4. Accordingly it was expected that Parotin might have direct stimulative effects on some sites of development of hard tissues in vivo.

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A new humoral factor of the anterior hypothalamus was found to possess a powerful activity in stimulating the liberation of hypophyseal thyrotrophin and enlarging the thyroid gland. This humoral factor seemed to be released from the hypothalamic-neurohypophyseal neurosecretory system in blood and urine. Administration of methylthiouracil provoked not only an enlargement of the thyroid but also a marked elevation of the neurosecretion of the Ncl. supraopticus or paraventricularis. On the contrary, thyroxin and chlorpromazine suppressed the release of pituitary thyrotrophin and hypothalamic neurosecretion.
It seemed likely that the thyrotroph-thyroidal function is under the control of the neurohumor of the anterior hypothalamic neurosecretory system.

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1. Intravenous injection of DHA (100mg) increased serum uric acid after 1hr., but injection of ACTH (10mg) had no effect on serum uric acid.
2. Daily injections of DHA (100mg per day) gradually increased serum uric acid level only for the period of injections.
3. Injection of DHA (100mg) and ACTH (5mg) apparently increased urinary uric acid excretion a day after injections.

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1. present study disclosed that relatively small amount of steroids are contained in the fraction of Cohn IV-1.
2. Steroids examined were of conjugated form with α-globulin or β-globulin, not being in free forms.
3. Such bound steroids were dissociated from the serum protein in the treatment with P. E. and isolated steroid moved to parts of IVp and Vp, which were characteristic of m-dinitrobenzene treatment, which disappeared in BPB staining, determined by Coleman's nepho-colorimeter after color development with m-DNB.
4. IVp and Vp represented the curves originated in androgenic steroid hormones and corticoid hormones respectively.

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The weight of the thyroidal gland increased by the use of methylthiouracil and was inhibited by chlorpromazine in experimental animals. The administration of MTU provoked not only an enlargement of the thyroid gland, but also an elevation of neurosecretion of the hypothalamic neurosecretory system. Thyroidectomy produced the most remarkable changes of the hypothalamic neurosecretory system. Chlorpromazine inhibited the hypothalamic neurosecretion and depressed the release of pituitary thyrotrophin. Thus the effect of methylthiouracil, chlorpromazine, and potassium iodide on the thyroid gland appears to be mediated through the hypothalamic neurosecretory system.

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