JAPANESE CIRCULATION JOURNAL
Online ISSN : 1347-4839
Print ISSN : 0047-1828
ISSN-L : 0047-1828
Volume 27, Issue 7
Displaying 1-8 of 8 articles from this issue
  • TADAO NORO
    1963 Volume 27 Issue 7 Pages 501-505
    Published: July 20, 1963
    Released on J-STAGE: April 14, 2008
    JOURNAL FREE ACCESS
    The propagation velocity of the pulse wave was examined in respect to age on the healthy children by obtaining the propagation time of the pulse wave of Saigusa et al at the finger-tip and calculating the velocity from this value and the length of the blood vessel.
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  • TADAO NORO
    1963 Volume 27 Issue 7 Pages 506-509
    Published: July 20, 1963
    Released on J-STAGE: April 14, 2008
    JOURNAL FREE ACCESS
    Author reported previously that the Propagation Velocity of the Pulse Wave increases as the years of age go up. In this report, as an application to clinic, the results of propagation velocity of the pulse wave evaluation in children with heart disease are mentioned.
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  • YOSHIAKI YASUDA
    1963 Volume 27 Issue 7 Pages 519-527
    Published: July 20, 1963
    Released on J-STAGE: April 14, 2008
    JOURNAL FREE ACCESS
    Various investigations have been performed about the pathogenesis of atherosclerosis, but no clear explanation has been presented so far. It has been suggested that atherosclerosis in man is often due to disturbed lipid metabolism, and in recent years β-lipoprotein, among the lipid fractions, has come to receive much attention for its role in the pathogenesis of atherosclerosis. The present paper describes a simple method of determining β-lipoprotein with dextran sulfate as reagent, and suggests that the level of β-lipoprotein determined by it may serve as an index of the severity of the disease. Method 1) The human subjects consisted of 465 normal persons, 114 patients with coronary insufficiency, 157 with hypertension, 42 with cerebrovascular lesions, 41 with diabetes mellitus, 66 with nephritis, and 29 with nephrotic syndrone. 2) In the animal experiment 28 male rabbits weighing 2 to 3 kg each were em-ployed. They were divided into two groups, one group being fed for a period of 90 days, and the other for 20 days. Each group was further subdivided into two, one subgroup being placed on an ordinary diet and the other on a lanolin-containing diet. 3) The serum β-lipoprotein level was determined with dextran sulfate as reagent. The turbidity produced when the reagent was added to the serum was measured with a Beckmann colorimeter, and the β-lipoprotein content was obtained by referring to a calibration curve prepared with standard solutions of known concentrations. In measuring the amounts of the other lipid fractions, modified Zak-Henly's method was employed for total cholesterol, alkali titration after extraction by Bloor and katsura-Hatakeyamas method for total fatty acid, modified Zilveramith's method for phospholipid, Bragdon's method for neutral fat, polarography after extraction by Grossman's method for non-esterified fatty acid. Results The coefficients of correlation between β-lipoprotein and total cholesterol, free cholesterol, total fatty acid, non-esterified fatty acid, phospholipid, and neutral fat were 0.73, 0.72, 0.75, 0.45, 0.52 and 0.59 respectively, showing pretty good positive correlation in every case.
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  • YOSHIAKI YASUDA
    1963 Volume 27 Issue 7 Pages 528-533
    Published: July 20, 1963
    Released on J-STAGE: April 14, 2008
    JOURNAL FREE ACCESS
    It was reported in the preceding paper that serum β-lipoprotein could be conveniently determined by the dextran sulfate method utilizing the affinity between β-lipoprotein and acid muco-polysaccharides, and that the level of the substance in serum was elevated in atherosclerosis. In this paper, a few experiments on the mechanism of the deposition of serum β-lipoprotein in the aortic wall are described. Methods and Results 1 ) Preparation of Anti-β-lipoprotein Serum Pure β-lipoprotein isolated from human serum by the dextran sulfate method was injected into rabbits and guinea pigs repeatedly to prepare anti-human-β-lipoprotein sera. The anti-sera were tested by agar immunoelectrophoresis and agar gel double diffusion, and were found to react specifically with β-lipoprotein. 2) Pressure Test of β-lipoprotein Experiments were performed to let serum albumin and β-lipoprotein invade a segment of the aorta of a dog or a pig placed in a specially designed apparatus. When pressure was applied to the solution of serum albumin labeled with bromphenol blue, the substance was found to invade the aortic wall to a depth approximately proportional to the pressure Next, human serum rich in β-lipoprotein was substituted for the solution, and after the pressurization the piece of aorta was removed and homogenized at low temperatures, and its β-lipoprotein extracted with physiological saline. The usual precipitation reaction between the extract and the anti-β-lipoprotein serum mentioned above showed the precipitin value to be approximately proportional to the pressure. The quantity of β-lipoprotein extracted was also measured by the dextran sulfate method and was found also to be roughly proportional to the pressure. The following experiments were carried out to test if it was due to combination with the acid muco-polysaccharides pressent in the aortic wall that the, β-lipoprotein was deposited there. First, the adventitia was removed from a piece of human aorta obtained in autopsy, and the media and intima were minced and freed of fat with acetone and ethyl ether, and dried. Then the acid muco-polysaccharides were isolated and purified by modified Kirk and Dyrbye's method and dissolved in distilled water. When human serum rich in β-lipoprotein was added to the solution, it became turbid, indicating combination of the acid polysaccharides with the β-lipoprotein.
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  • HARUHIKO OTANI
    1963 Volume 27 Issue 7 Pages 534-546
    Published: July 20, 1963
    Released on J-STAGE: April 14, 2008
    JOURNAL FREE ACCESS
    Many reports have been made public on cerebral blood flow and cerebral metabolism since methods of measuring cerebral blood flow were established, but systematic investigations on cerebral carbohydrate metabolism are few and especially those concerning the influence of hypoxemia are not found in the literature. The present investigation was designed with these problems in mind. Methods and Materials A total of 27 cases including healthy controls and patients with pulmonary tuberculosis, hypertension or cerebral arteriosclerosis were used. A catheter was introduced into the bulbus venae jugularis interna and the cerebral bood flow was determined by the N2O method. The carbohydrates of the arterial and internal jugular venous bloods were determined. These determinations were done during a resting state and during hypoxemia induced by 10% O2 inhalation for 20 minutes.
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  • HARUHIKO OTANI
    1963 Volume 27 Issue 7 Pages 547-561
    Published: July 20, 1963
    Released on J-STAGE: April 14, 2008
    JOURNAL FREE ACCESS
    Many reports have been made public on cerebral blood flow and cerebral metabolism since methods of measuring cerebral blood flow were established, but systematic investigations on cerebral carbohydrate metabolism are few and especially those concerning the influence of hypoxemia are not found in the literature. The present investigation was designed with these problems in mind. Methods and Materials A total of 27 cases including healthy controls and patients with pulmonary tuberculosis, hypertension or cerebral arteriosclerosis were used. A catheter was introduced into the bulbus venae jugularis interna and the cerebral bood flow was determined by the N2O method. The carbohydrates of the arterial and internal jugular venous bloods were determined. These determinations were done during a resting state and during hypoxemia induced by 10% O2 inhalation for 20 minutes.
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  • TAKU OKUI
    1963 Volume 27 Issue 7 Pages 562-567
    Published: July 20, 1963
    Released on J-STAGE: April 14, 2008
    JOURNAL FREE ACCESS
    Eye ground findings recorded by the criteria of Keith-Wagener or Scheie in essential hypertension and cerebral arteriosclerosis, have proven to be of much value clinically. However, these observation are mostly morphological and do not express were hemodynamic changes. Studies in our department have been centered around the vessel reaction of verious organs caused by induced hypoxemia and the findings concerning the coronary, cerebral, hepatic, and renal circulations have been reported previously. Recently, the determination of retinal blood flow has been established by ophthalmologists and this prompts us to study the hemodynamics of the retinal circulation during the resting state and during induced hypoxemia with the purpose of investigating the pathophysiology of the retinal circulation and the possibility of advancing a step towards the diagnosis of retinal vessel function. Methods and Materials A) Methods used : Values concerning retinal hemodynamics during the resting state were calculated by measuring the brachial and retinal blood pressures and eye ground photography. Immediately after those Procedures a mixture of 10% O2 and 90% N2 was administered for 20 minutes and the above procedures were repeated to determine the retinal hemodynamics during the induced hypoxemia. B) Calculations i) Retinal blood flow was calculation by the Hagen-Poiseuille law. ii) Comparison of central retinal artery and brachial artery blood pressures. iii) Systolic blood pressure ratio iv) Diastolic blood pressure ratio v) Pulse pressure ratio vi) Further relative differential pressure ratio
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  • TAKU OKUI
    1963 Volume 27 Issue 7 Pages 568-573
    Published: July 20, 1963
    Released on J-STAGE: April 14, 2008
    JOURNAL FREE ACCESS
    Eye ground findings recorded by the criteria of Keith-Wagener or Scheie in essential hypertension and cerebral arteriosclerosis, have proven to be of much value clinically. However, these observation are mostly morphological and do not express were hemodynamic changes. Studies in our department have been centered around the vessel reaction of verious organs caused by induced hypoxemia and the findings concerning the coronary, cerebral, hepatic, and renal circulations have been reported previously. Recently, the determination of retinal blood flow has been established by ophthalmologists and this prompts us to study the hemodynamics of the retinal circulation during the resting state and during induced hypoxemia with the purpose of investigating the pathophysiology of the retinal circulation and the possibility of advancing a step towards the diagnosis of retinal vessel function. Methods and Materials A) Methods used : Values concerning retinal hemodynamics during the resting state were calculated by measuring the brachial and retinal blood pressures and eye ground photography. Immediately after those Procedures a mixture of 10% O2 and 90% N2 was administered for 20 minutes and the above procedures were repeated to determine the retinal hemodynamics during the induced hypoxemia. B) Calculations i) Retinal blood flow was calculation by the Hagen-Poiseuille law. ii) Comparison of central retinal artery and brachial artery blood pressures. iii) Systolic blood pressure ratio iv) Diastolic blood pressure ratio v) Pulse pressure ratio vi) Further relative differential pressure ratio
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