The change in the structure of regenerated scales was pursued in this study, which were formed where all the scales on one side of the body were taken off with a pincette.
In regenerated scales in the early stage of regeneration, grooves, which form network, are spread all over the scale, and ridges are not observable yet. As regeneration goes on, network grooves gradually disappear from around the circumference of scales and radial grooves decrease in number, while ridges increase in number by degrees; then the scales gradually come nearer to the structure of ordinary scales, until at last they are utterly indistinguishable from ordinary ones, in the shortest period of 180 days.
When a half of each scale is cut off with scissors, the part cut off is soon regenerated, where many grooves are formed in the early stage and ridges are not seen, but as days pass, grooves decrease in number, while ridges increase in number.
The speed of regeneration varies as the season changes; it is fast in summer, the scales recovering the structure of complete regenerated scales in about 15 days, and slow in winter, at least in more than a month.
According to this study, it is supposed that regenerated scales, in the early stage of regeneration, are all strikingly different in structure from ordinary ones, but that as they go on growing, they come nearer to the structure of ordinary scales to be finally indistinguishable from them.
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