The artificial fertilization of the puffer,
Takifugu chrysops (Hilgendorf), was carried out at Sajima in Yokosuka City on May 22, 1984. Hatched larvae were reared for a period of about 150 days. The spawning period seems to extend from mid to late May in the eastern part of Sagami Bay. The eggs were spherical, pale milky white and semitransparent, demersal and adhesive in nature, measuring 1.32±0.04mm in diamter, and with a cluster of small oil-globules.The incubation period was about 162 hours at a water temperature of 17.4 to 21.8°. During embryonic development, the only pigment cells that appeared on the embryo were the black chromatophores. The newly hatched larvae measured from 2.72 to 3.06mm TL, averaging 2.87±0.1mm TL, and 22-23 (9+13-14) myomeres. At yolk absorption, 4days after hatching, the larvae attained 3.64-3.79mm TL. On the 11th day, postlarvae averaged 4.69±0.24mm TL. Larval finfolds disappeared and rudimental dorsal, anal and caudal fins were formed. There were two large clusters of melanophores, one on the back, exteding from the mid-base of the dorsal fin to the caudal peduncle region, the other along the anal fin base. The color of the body began to turn pale green to brownish-orange and spinelike scales appeared on the belly. Eighteen days after hatching (7.02±0.27mm TL), the caudal notochord began to turn up and a “constriction” appeared on the posterior margin of the caudal fin membrane. This notch moved upwards as the notochord upturning advances. The larvae attained full fin ray counts and reached the juvenile stage at 9.1-9.5mm TL, 24 days after hatching. Characteristic black blotches on the back and specific brownish orange body color appeared at the stage of 20mm TL, 24 days after hatching.
The growth during the larval stage and early juvenile stage (24 to 51 days after hatching) were expressed by the following equations, where y is total length (mm) and x is days after hatching.
y1=2.8424×1.0509
x (0≤
x≤24)
y2=3.7872×1.0372
x (24≤
x≤51)
View full abstract