We studied changes in the 2-mercaptoethanol (2 ME) sensitive antibody level in the hemoagglutinin inhibition (HI) reaction positive sera of wild birds, chicken and the patient after natural infection with Japanese encephalitis virus, and also localization of 2 ME sensitive antibody in serum fractions. The results were as follows.
1) 2 ME sensitive antibody was present in 6 out of the 11 night herons studied, one of the 6 white herons, and 6 of the 7 chicken, in August through October, 1966.
2) HI antibody in the chicken was proved to exist in γ-globulin fraction by starch block electrophoresis.
3) 2 ME sensitive antibody in the chicken decreased with the lapse of time after infection accompanied by increase of resistant antibody.
4) HI antibodies composed of 2 ME sensitive antibody and 2 ME resistant antibody in the chicken were found to be contained in 2 kinds of serum fractions out of the three by Sephadex G-200 gel filtration.
5) From the above results, in items 3 and 4, we cannot necessarily ignore chicken as amplifier of Japanese encephalitis virus.
6) HI antibody in the patients' sera containing abundant 2 ME sensitive antibody was proved to exist in the first fraction containing 19 S antibody by Sephadex G-200 gel filtration.

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For the purpose of studying the diagnostic significance of the increased percentage of beta globulin in the lumbar cerebrospinal fluid, the fluid in the intracranial subarachnoid space which seemed to contain a large percentage of beta globulin was investigated electrophoretically and immunologically. Specimens were obtained at the time of spinal tap for pneumoencephalography from 30 patients who were mentally and neurologically ill.
1) A higher percentage of beta globulin in the intracranial subarachnoideal fluid seemed to be physiological.
2) The large percentage of beta globulin in the lumbar fluid was in close association with subarachnoideal fluid.
3) The lumber fluid was not a simple mixture of two fluids: 1) fluid in the ventricles which contained larger amounts of prealbumin and 2) fluid in the intracranial subarachnoid space which contained larger amounts of beta globulin.
4) The beta globulin in cerebrospinal fluid could be divided into beta-1 (transferrin) and beta-2 (taufraction) globulins. The concentration of tau-fraction was greater in subarachnoideal fluid than in lumbar and ventricular fluid.
5) The immunological survery revealed the fact that the transferrin in cerebrospinal fluid was derived from serum.
6) A possible explanation for diagnostic significance of the observed phenomena was discussed.

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