We take some technical considerations in mikroelectrophoresis for cells. Details of construction of a mikroelectrophoretic apparatus, suitable for study of living cells, are given. The problem of measuring mobility is discussed. Electrophoretic mobility of the red blood cells of healthy persons in various buffer solution are presented.

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Plasmapheresed and nontreated rabbits were injected with tritiated albumin intravenously. Analysis of serum proteins and distribution of tritium were studied before and after injection.
1) More than 99% of tritiated albumin has disappeared from plasma at the short time interval after the injection. After this rapid diminution, the level of tritium has decreased gradually.
2) Level of tritium found per gram of globulin was 8-80 times more than that of albumin right after the injection. Radio activity of tvi tium Per gram of nitrogen was higher innonprotein nitvcgen than in albumin.
3) With increasing in time after the injection, the specific activity of tritium globulin has decreased rapidly and at 6-24 hours after the injection the level of tritium per gram of globulin was about the same to that of albumin.
4) From the above data, injected albumin seems to be absorbed into the tissues and the part of tritium may be incorporated into globulin.

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Two-dimentional crossing paper electrophoreses of human blood serum against Protein J contained in the extract from Jack bean were carried out, and the changes in the “crossing diagram” obtained from 159 patients with various diseases were studied in comparison with those of 40 normal subjects.
1) Human sera, except in some diseased cases, showed crossing diagrams consisting of three peaks, which correspond to three globulin fractions, α₁-, α₂- and β-globulin. The peaks were formed due to the precipitation reaction between Protein J and these serum proteins.
2) Sera from 58 patients showed crossing diagrams which can not be differentiated from those of normal sera. On the other hand, sera from 101 patients showed clearly different crossing diagrams from those of normal subjects. The changes in the crossing diagrams were classified into 7 types.
3) Sera from 5 patients (including two multiple myeloma, one leucosarcoma, one gastric ulcer with gastric ffbroma, and one liver cirrhosis) showed crossing diagrams with an unusual fourth peak besides the three.
4) Specific correlation between the type of the changes in crossing diagrams and the diseases was hardly observed, although in nephrotic syndrome diagrams were marked with pronounced elevation of α₂ peak.
5) Relations between the relative concentrations of serum α₁-, α₂- and β-globulin fractions determined by the usual paper electrophoresis and the changes in crossing diagrams were investigated. In most cases they could be correlated, but in some cases correlation could not be observed.

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Two-dimentional crossing paper electrophoreses of sera from 60 patients with malignant tumors against Protein J of Jack Bean were carried out. The changes in the “crossing diagram” obtained were compared with those obtained from sera of 23 cases with benign tumors and 20 cases with diseases of stomach and duodenum without tumor. Furthermore, relationship between the relative concentrations of α₁-, α₂- and β-globulin fractions of the sera and the changes in crossing diagrams was investigated in the cases with malignant tumors.
Following results were obtained:
1) In an overwhelming majority, the cases with malignant tumors showed distinctly different crossing diagrams from those of normal subjects. And in the greater part of those which showed changes in the crossing diagrams was observed elevation of α₂ peak or lowering of β peak.
2) Specificity of the type of the changes in crossing diagrams obtained from patients with malignant tumors was not distinct.
3) In the cases of malignant tumors, elevation of α₂ peak in diagrams was more often and was larger than that in the cases with benign tumors.
4) The same as above held also in the cases of gastric cancer compared with those of peptic ulcer and chronic gastritis.
5) In many of the cases of malignant tumors, the relative concentrations of α₁-, α₂- and β-globulin fractions and the changes in the crossing diagrams could be correlated, but in some cases not.

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Two-dimentional crossing paper electrophoreses of sera from patients were carried out against Protein J of Jack Bean, and the changes in the “crossing diagrams” obtained were studied.
1) Crossing diagrams obtained from 5 patients presented here showed an unusual fourth peak, besides the usual three which correspond to α₁-, α₂- and β-globulin fractions.
2) In two cases of multiple myeloma, a fourth peak were observed in crossing diagrams corresponding to myeloma protein.
3) In one case of gastric ulcer with gastric fibroma, a very low fourth peak was observed in crossing diagram corresponding to an abnormal protein fraction located between the β- and γ-globulins.
4) In each one case of leucosarcoma and advanced portal liver cirrhosis, a fouth peak was observed in crossing diagram between the β- and γ-globulin fractions, although in electrophoretic protein patterns of those sera unusuol protein fractions were not demontrated.
5) Specific correlation between the appearance of the fourth peak in crossing diagram and the diseases was not observed.

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[in Japanese]

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