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Volume 39, Issue 1
Displaying 1-8 of 8 articles from this issue
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effect of constant current and constant voltage on cellulose acetate membrane electrophoresis using Separax SPJunko Inoue, Hiroko Cho, Kiyoko Shiba, Takashi Yoshida1995 Volume 39 Issue 1 Pages 1-4
Published: February 15, 1995
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSCellulose acetate membrane electrophoresis was performed at a constant current and constant voltage using a new cellulose acetate membrane, Separax SP, which is slightly electro-osmotic. The rate of evaporation flow was obtained by observing the movement of Vitamin B12 which had been applied to the cellulose acetate membrane. Evaporation flow was smallest in the inner part of the membrane of electrophoretic axis and largest towards the outer part. The evaporation rate was not affected by changes in the current or voltage. However, it was dependent on the total power. Separax SP is usually considered not to be electro-osmotic. However, application of an alternating current revealed electro-osmotic properties. Even when total electrical power was kept constant, the electrophoretic patterns of the serum proteins differed, depending on whether we used constant current or constant voltage for run. These differences are attributable to electrical changes, during electrophoresis. With constant voltage, a small amount of electricity is applied during the early stages of electrophoresis and a large amount of electricity is the finishing stages. This provides a better separation of the samples.View full abstractDownload PDF (3678K) -
Mayumi Imoto, Kazuhiko Yamamoto, Hyogo Sinohara, Ikunosuke Sakurabayas ...1995 Volume 39 Issue 1 Pages 5-12
Published: February 15, 1995
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSWe reported a rare case of hyperviscosity syndrome with IgA1-λ type M protein. Hyperviscosity was due to complex formation between patient's abnormal IgA1 and fibrinogen. Gel formation of patient's plasma occurred during withdrawal of venous blood. The α1 heavy and λ light chains of this purified abnomal IgA1 were found to have the same molecular weight as the normal IgA. Isoelectric points of abnormal α1 heavy chain ranged from 4.6 to 5.2 which were changed toward the neutral pH upon treatment with sialidase. Since the complex formation was reversible depending upon the temperature, the association between abnormal IgA1 and fibrinogen seems to be noncovalent. As a result of analysis of amino acid sequence, 16th amino acid of CH1 domain changed to cystein from tyrosine. These data suggest that amino acid sequence or abnormality of second structure in the variable region of patient's IgA differs from that of nomal IgA, and that these abnormalities are concerned with association with fibrinogen.View full abstractDownload PDF (7578K) -
Mieko Miyoshi, Masayuki Kobayashi, Katsuhiko Machida, Ikunosuke Sakura ...1995 Volume 39 Issue 1 Pages 13-18
Published: February 15, 1995
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA patient with IgA-κ type multiple myeloma was found to have free μ heavy chain in his serum. The molecular weight of the free μ heavy chain was about 65, 000 dalton, and was not associated with any type of light chain, and Bence Jones protein (κ type) was detected in the patient serum and urine. Atypical plasma cells in the bone marrow did not show vacuoles nor inclusion bodies in the nucleus and cytoplasm. A few case was reported that detected heavy chain fragment in patients without heavy chain disease; γ heavy chain fragment in a patient with IgG type multiple myeloma, α heavy chain in a patient with unusual lymphoadenopathy, μ heavy chain in a patients with macroglobulinemia, generalized lymphoadenopathy, and plasma cell dyscrasia. This case was compatible with these reported cases. It is conceivable an abnormal heavy chain is detectable in various pathological condition, especially B cell type abnormalities, not limiting the heavy chain diseases.View full abstractDownload PDF (4806K) -
Chieki Wada, Shigeru Shionoya, Toyoaki Uchida, Hideki Ohtani1995 Volume 39 Issue 1 Pages 19-23
Published: February 15, 1995
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSGenomic instability in sixteen chronic myelogenous leukemia (CML) patients clinically progressing from the chronic phase to blast crisis was analyzed by a PCR-microsatellite instability assay (MIA). Instability in five separated microsatellites was examined and the difference in microsatellites of chronic and blast crisis DNAs in 13 of 16 patients (81.3%) were demonstrated. In 9 of 16 cases (56.3%), genomic instability in at least two of five microsatellites was observed and these blast crises were categorized as DNA replication error (RER+) phenotypes. These highly frequent RER+ may be closely associated with the transition from chronic to blast crisis of CML. By PCR-MIA, microsatellite instability may be a useful molecular marker for monitoring of CML blast crisis.View full abstractDownload PDF (3281K) -
Kazuo Umetsu, Isao Yuasa, Tsuneo Suzuki1995 Volume 39 Issue 1 Pages 25-29
Published: February 15, 1995
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSThis study was made to explore the cause of appearance of unusual variant bands of vitamin D-binding protein (group-specific component, GC). Both GC 1A2 and GC 1A3 have two main bands and two minor bands, and both variants were characterized by having cystein instead of arginine at the codon 429. The minor bands disappeared by keeping them at 37°C, or adding CuSO4 or DTT to them. The results indicated that the characteristic main bands of GC 1A2 and GC 1A3 are derived from the oxidation of cystein (SH group) which corresponds to cystein at the codon 429.View full abstractDownload PDF (4010K) -
Shigenori Ikemoto, Shuichi Tsuchida1995 Volume 39 Issue 1 Pages 31-35
Published: February 15, 1995
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSRestriction fragment length polymorphism (RFLP) analysis was carried out in a case of bone marrow transplantation, in which a pair of serological siblings represented an unexpected MLC reactivity. The RFLP patterns detected using the probes consisted of HLA class II gene cDNA showed the differences between the donor and the recipient. The unexpected MLC reactivity observed between the donor and recipient with the identical serological HLA types appeared to be induced by the HLA class II disparities which was detectable by DNA analysis.View full abstractDownload PDF (1583K) -
Toshio Okazaki, Yuko Yanagisawa, Tatsuo Nagai1995 Volume 39 Issue 1 Pages 37-39
Published: February 15, 1995
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (1495K) -
Toshio Okazaki, Yuko Yanagisawa, Tatsuo Nagai1995 Volume 39 Issue 1 Pages 41-43
Published: February 15, 1995
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (1744K)
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