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Volume 26 (1982)
- Issue 6 Pages 411-
- Issue 5 Pages 333-
- Issue 4 Pages 259-
- Issue 3 Pages 159-
- Issue 2 Pages 71-
- Issue 1 Pages 1-
Volume 26, Issue 6
Displaying 1-4 of 4 articles from this issue
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1982 Volume 26 Issue 6 Pages 411-449
Published: December 25, 1982
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (12324K) -
I: Purification of IgD and preparation of antiserumMasaaki Arakawa, Hiromitsu Enomoto, Yoshihisa Ito, Ikunosuke Sakurabay ...1982 Volume 26 Issue 6 Pages 451-456
Published: December 25, 1982
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA new method was established for a high yield of purified IgD by using ion exchange chromatography under the conditions where the IgD protein was not absorbed.
Despite the buffer containing 0.01M ε-amino caproic acid was used to protect against proteolysis, spontaneous degradation of IgD occured during purification step at 4°C. The molecular weight of this degradation fragment was about 123, 000 daltons determined by SDS-PAGE. It was, therefore, probable that the fragments were F (ab)2δ or the dimer of Fcδ and Fabδ.
For the study of the reactivity aginst Fabδ portion, specific rabbit anti-IgD antibody was prepared and compared with those supplied commercially. The results indicated that the latter did not show any reaction against Fabδ, suggesting that it may lack the antibody against Fdδ.View full abstractDownload PDF (5057K) -
Keiko Manabe, Mitsuhisa Manabe, Kayoko Sudo, Takashi Kanno1982 Volume 26 Issue 6 Pages 457-462
Published: December 25, 1982
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSThe physico-chemical and kinetical properties of abnormal pseudocholinesterase (p-ChE) in two families (J. T. and K. K.) were studied and discussed.
The p-ChE levels of the patient J. T. and K. K. were 10% and 3% of the lower limit of normal ranges, respectively. The p-ChE of patient J. T. was classified as homozygote for atypical gene by the inhibition studies. However, the p-ChE of K. K. showed much larger Km-values for several substrates than normal controls and the Hill's number of the patient's p-ChE was 1.10, though the values less than 1.0 were usually obtained from normal control. Therefore, the p-ChE of K. K. have not the allosteric property with negative cooperativity, as was the nature of normal one. Further studies by isoelectric focusing and slab PAG electrophoresis revealed the traces of the activity of p-ChE in the patient J. T., but any activity band was not detected in the patient K. K. These results indicated that the patient J. T. was homozygote of atypical gene and that the patient K. K. was homozygote for silent gene. It was suggested that the analysis of Hill's number, additional to the conventional inhibition studies, might be useful for the detection of the silent gene of p-ChE in details.View full abstractDownload PDF (4247K) -
Yasuhiko Sakoyama1982 Volume 26 Issue 6 Pages 463-468
Published: December 25, 1982
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSOvary proteins in 2 normal and 38 maternal effect mutants of Drosophila melanogater were analysed by two-dimensional polyacrylamide gel electrophoresis. Approximately 300 protein spots were detected in the ovary and the embryo samples, of which 140 were also found in adult somatic tissues both in the head and in the thorax. Among 38 maternal effect mutants studied, some showed changes in protein patterns which probably reflected allelic differences. In no cases, however, changes were reflected to the maternal effect mutant genes.View full abstractDownload PDF (5456K)
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