SEIBUTSU BUTSURI KAGAKU
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
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Volume 6, Issue 4
Displaying 1-8 of 8 articles from this issue
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  • Arteriosclerosis and Lipoprotein
    Kiiti Oji
    1960 Volume 6 Issue 4 Pages 209-218
    Published: July 15, 1960
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.6.209
    JOURNAL FREE ACCESS
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  • Arteriosclerosis and Lipoprotein
    Noboru Kimura, [in Japanese]
    1960 Volume 6 Issue 4 Pages 219-224
    Published: July 15, 1960
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.6.219
    JOURNAL FREE ACCESS
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  • Studies on Serum Lipoproteins
    Kenzo Oshima, [in Japanese], [in Japanese]
    1960 Volume 6 Issue 4 Pages 225-230
    Published: July 15, 1960
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.6.225
    JOURNAL FREE ACCESS
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  • Biochemical Studies on the Pathological Synovial Fluid
    Tomosada Mashiko
    1960 Volume 6 Issue 4 Pages 231-240
    Published: July 15, 1960
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.6.231
    JOURNAL FREE ACCESS
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    Biochemical studies concentrating on a paper electrophoretical analysis were made with the following results on the synovial fluid in an attempt to make a diagnosis of arthropathy.
    1. The amount of protein was greater than that in a control group and was proportional to the intensity of the inflammation.
    2. The severer the inflammation was, the lower was the viscosity of the synovial fluid. The viscosity indicated the local findings well.
    3. The flocking number reaction (Takata) and particularly the mat delayed reaction were of value in observing the course of diseases.
    4. Although the Tokata reaction, the Chinin reaction and the cadmium reaction did not occur in the synovial fluid the clinical significance of the Weltmann's coagulation band for serum was recognized.
    5. A marked increase in mucoprotein took place in patients blood serum with rheumatosis. This finding was of significance in following the course of the diseases.
    6. No changes between the types of disease took place in alkali phosphatase and in inorganic substances Na, K, P and Ca.
    7. Amarked decrease of A/G ratio was observed in rheumatoid arthritis which was proportional to the intensity of inflammation.
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  • Studies on a New Method of Zone-Electrophoresis
    Report 2. Comparisons of electrophoretic diagram in two or three kinds of buffer
    Tsunetaka Kushimoto
    1960 Volume 6 Issue 4 Pages 241-248
    Published: July 15, 1960
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.6.241
    JOURNAL FREE ACCESS
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    Electrophoretic diagram of human plasma was obtained with the apparatus using electrophoresis under density gradiet of dextran. Plasma protein was separated into sixteen fractions by either veronal buffer at pH 8.6 and ionic srength 0.1 or sodium bicanate buffer at pH 8.37 and ionic strength 0.05. While, only ten clearly separated fractions were detectable with phosphate buffer at pH 7.8 and ionic stength 0.144. Quantity and mobility of each fraction were determined and the result was disscussed in relation to that obtained by Tiselius apparatus.
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  • Pre-staining Procedure for Electrophoretic Study of Serum-Lipoprotein with acetylated Sudan black B
    Hitoshi Itoh, Isao Miura, Fumihiko Mori, Yukio Ishiwara, Shigeo Kaizaw ...
    1960 Volume 6 Issue 4 Pages 249-253
    Published: July 15, 1960
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.6.249
    JOURNAL FREE ACCESS
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    Paper electrophoresis is one of the most convenient methods for the study of serum lipoproteins. In spite of vast amounts of papers published, there is still many difficulties in the staining prncedure of lipid moiety of lipoprotein, e. g. poor staining, long time pecquired for staining, procedure and strong adsorption of dye to the paper itself. The author tested the pre-staining procedure using acetyl sudan black B prepared by the method of Lillie and Burtner. 0.1ml of saturated solution of the dye in ethanol was added to 1.0ml of serum before application of the serum on filter paper. Both alpha and beta lipoproteins migrate in a usual manner and appear much clear as blue zones against a white background.
    Examination on the duration of staining, amount of sample, duration of electrophoresis the authors found that the best results could be obtained under the following conditions: buffer; veronal pH, 8.6; ionic strength 0.05; potential gradient; 10volt/cm; amount of sample 0.025-0.035ml/1.5cm; duration of electrophoresis 2-3 hours.
    Comparing with Swahn's original method, pre-staining procedure reported here was foud to be better in reproducibility, time and cost.
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  • Normal Value of Serum Protein Fractions by Fractionating Elution Method
    Toshiya Tatibana, [in Japanese], [in Japanese], Ryoiti Sugimoto, Akira ...
    1960 Volume 6 Issue 4 Pages 254-257
    Published: July 15, 1960
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.6.254
    JOURNAL FREE ACCESS
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  • Notes on the Standardization of Densitometer for Paperelectrophoresis
    Kazuo Shimao, Ryoiti Sugimoto, Akira Miyamoto, Toshio Asada, Kaizo Ari ...
    1960 Volume 6 Issue 4 Pages 258-261
    Published: July 15, 1960
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.6.258
    JOURNAL FREE ACCESS
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