SEIBUTSU BUTSURI KAGAKU
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
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Volume 49, Issue 4
Displaying 1-4 of 4 articles from this issue
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  • Analysis of latent mesangial IgA deposition in renal allograft donors
    Koichi Suzuki, Takahito Moriyama, Kosaku Nitta
    2005 Volume 49 Issue 4 Pages 97-99
    Published: December 15, 2005
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.49.97
    JOURNAL FREE ACCESS
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    Immunoglobulin A (IgA) nephropathy (IgAN) is now considered the most common form of primary glomerulonephritis worldwide. It is characterized by predominantly mesangial IgA and C3 deposition, which is related to mesangial proliferative changes. Mesangial IgA deposition is incidentally encountered in asymptomatic individuals, but its precise frequency and significance had not been clarified. We performed zero-hour allograft biopsies in 510 renal transplantations (446 living donors, and 64 cadaveric donors) at the Kidney Center of Tokyo Women's Medical University. Mesangial IgA and C3 deposition were analyzed immunohistochemically, and the frequency and pathological features of mesangial IgA deposition were investigated. Mesangial IgA deposition was present in 82 (16.1%) of the total 510 allografts with no statistical difference between living donors (72/446, 16.1%) and cadaveric donors (10/64, 15.6%) or between blood-related donors (66/392, 16.8%) and nonblood-related donors (16/110, 14.5%). Histologic investigation of IgA(+) allografts revealed the frequency of mesangioproliferative glomerulonephritis was significantly higher in IgA(+)/C3(+) allografts (8/16, 50%) than in IgA(+)/C3(-) allografts (11/66, 16.7%). Moreover, the number of infiltrated macrophages to glomerulus (cells/glomerular cross section) was significantly higher in the IgA(+)/C3(+) allografts than in IgA(+)/C3(-), IgA(-)/C3(+) and IgA(-)/C3(-) allografts. The latent mesangial IgA deposition was a relatively common phenomenon in the healthy Japanese donors. This phenomenon was associated with mesangial proliferation and glomerular macrophage infiltration in some of the affected individuals, especially with combined IgA and C3 deposition. The results should provide insights into the epidemiology and pathogenesis of IgAN, the most common form of glomerulonephritis in many countries.
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  • IgA nephropathy: Approach by cellulose acetate membrane electrophoresis
    Ryoko Machii
    2005 Volume 49 Issue 4 Pages 101-105
    Published: December 15, 2005
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.49.101
    JOURNAL FREE ACCESS
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    The width of the albumin fraction in IgA nephropathy (IgAN) urine before treatment was significantly expanded by cellulose acetate membrane electrophoresis with colloidal silver stain. This phenomenon was not shown in IgAN urine after treatment or non-IgAN urine. There was a reverse correlation between the width of the albumin fraction and the albumin concentration in urine among these diseases. By immunofixation, Tamm-Horsfall protein was located in the same position as the albumin band in IgAN urine before treatment, but in the urine of a healthy subject, it was located in the same position as α1-globulin. By ELISA, the THP-albumin complex concentration in IgAN urine before treatment was significantly higher than that in the other two diseases. The width of the albumin fraction and the sodium ion concentration in the urine was significantly correlated. THP/albumin ratio in IgAN urine before treatment was significantly higher than that of the other two groups. This suggested that the characteristic expanded width of albumin found by immunofixation is a THP-albumin complex, and the sodium concentration in urine was involved in the THP-albumin complex formation.
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  • The application of electrophoresis as preparative tools for the identification of proteins by mass spectrometry
    Toyofumi Nakanishi
    2005 Volume 49 Issue 4 Pages 107-110
    Published: December 15, 2005
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.49.107
    JOURNAL FREE ACCESS
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    We have been found some disease-related proteins such as abnormal Hbs, variant transthyretins (TTR: hereditary amyloid polyneuropathy) and superoxide dismutase-1 (SOD-1: familial amyotrophic lateralsclerosis) by electrophoresis/soft ionization mass spectrometry. As you had already known, there are autoantibodies to cancer specific antigens in plasma of cancer patients and various angiogenetic regulated factors in vitreous humors of patients with diabetes mellitus. By detections and identifications of these molecules give useful information to diagnose of cancer, diabetic retinopathy or development of an immunity treatment. In this study, we applied the electrophoresis as a preparative tool of various samples prior to the analysis of proteins by mass spectrometry.
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  • A new analytical method for serum lipoproteins by gel permeation high performance liquid chromatography
    Mitsuyo Okazaki
    2005 Volume 49 Issue 4 Pages 111-118
    Published: December 15, 2005
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.49.111
    JOURNAL FREE ACCESS
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