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Volume 43, Issue 4
Displaying 1-7 of 7 articles from this issue
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Nobuya Hashimoto1999 Volume 43 Issue 4 Pages 199-201
Published: December 15, 1999
Released on J-STAGE: March 31, 2009
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Johann Bauer, Wolfgang Schütt, Domagoj Sabolovic, Nobuya Hashimot ...1999 Volume 43 Issue 4 Pages 219-223
Published: December 15, 1999
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSAt ICES '99 particular emphasis was laid on electrophoresis of cells and particles dedicating a complete session to this topic. Challenged by this organizational strategy of Prof. Hashimoto 16 highly interesting abstracts had been submitted, of which 8 were presented orally. All of them showed that analytical as well as preparative electrophoresis of cells and particles have already reached a very high level of performance. Some possibilities of improving resolution were still proposed regarding analytical (3 abstracts) as well as preparative (2 abstracts) carrier free electrophoresis devices. All the other presentations, however, clearly demonstrated which tremendous impact on research and even on commercial production electrophoresis of cells and particles currently has. Outstanding results in chemical, biochemical and medical research were explained which would not have been obtained without carrier free electrophoresis technologies.View full abstractDownload PDF (947K) -
Shigeru Terabe, Joselito P. Quirino1999 Volume 43 Issue 4 Pages 225-231
Published: December 15, 1999
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSMicellar electrokinetic chromatography (MEKC) as well as other capillary electrophoretic modes suffers from low concentration sensitivity due to low sample volume and limited pathlength for on-capillary photometric detection. On-line sample concentration techniques have been developed by simple manipulation of sample and background solution constituents without alteration of MEKC instrumentation, which are sample stacking and sweeping. These focusing techniques provide from 10 to more than 5000-fold improvement of the detector response. The enhancements are equivalent to 1-3 orders of magnitude improvement in the limit of detection. They are simple and are easily transferable technologies.View full abstractDownload PDF (5928K) -
Tsugikazu Komoda1999 Volume 43 Issue 4 Pages 233-241
Published: December 15, 1999
Released on J-STAGE: March 31, 2009
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Tosifusa Toda1999 Volume 43 Issue 4 Pages 243-244
Published: December 15, 1999
Released on J-STAGE: March 31, 2009
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V. Computer simulation of sodium dodecyl sulfate-polyacrylamide gel electrophoresisKazuo Shimao1999 Volume 43 Issue 4 Pages 245-250
Published: December 15, 1999
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSThe behaviors of all the components of glycine and tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis system in the course of separation of polypeptide-dodecyl sulfate complexes are investigated by computer simulation. In both of the systems, the leading chloride ion zone is followed by concentrated dodecyl sulfate zone, the length of which gradually increases with the time of electrophoresis. Under the postulated conditions and the values of physico-chemical parameters of the components of the systems, almost all the polypeptides migrate as the dodecyl sulfate-polypeptide complexes. These complexes are stacked between the dodecyl sulfate zone and the terminating glycine or tricine zone when they reach the separation gel. The high mobility complexes are not overtaken by the terminating glycine in the separation gel but stacked between the dodecyl sulfate and the glycine zone, while, all the complexes are overtaken in the separation gel by the terminating tricine and separated in the separation gel. Thus, the reason for the usefulness of tricine for the separation of the low molecular complexes is clarified by the computer simulation.View full abstractDownload PDF (709K) -
Junko Fujiwara, Young Moo Lee, Junko Satoh, Katsumi Yoshida, Takeshi S ...1999 Volume 43 Issue 4 Pages 251-254
Published: December 15, 1999
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA non-radioisotopic PCR-SSCP method using fluorescently labeled primers was developed for use in a clinical setting. In order to establish PCR-SSCP method for the clinical use, we developed a non-radioisotopic PCR-SSCP method using fluorescence-labeled primers. Electrophoresis in buffer containing with MgCl2 provided good separation of the bands and use of an image analyser gave more sensitive detection than conventional method. The mutations of K-ras gene and androgen receptor gene were confirmed by DNA sequencing analysis of the known mutations of K-ras gene and the unknown mutations of androgen receptor gene. The fluorescence-mediated PCR-SSCP system made it possible to detect mutations more rapidly and with more sensitivity than conventional PCR. Thus, the improved PCR-SSCP method will be useful for the clinical screening of unknown mutatios.View full abstractDownload PDF (4326K)
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